Hepatology,
Journal Year:
2023,
Volume and Issue:
80(3), P. 698 - 720
Published: April 1, 2023
Single-cell
transcriptomics
enables
the
identification
of
rare
cell
types
and
inference
state
transitions,
whereas
spatially
resolved
allows
quantification
cells
genes
in
context
tissues.
The
recent
progress
these
new
technologies
is
improving
our
understanding
landscape
its
roles
diseases.
Here,
we
review
key
biological
insights
into
liver
homeostasis,
development,
regeneration,
chronic
disease,
cancer
obtained
from
single-cell
transcriptomics.
We
highlight
atlas
that
characterizes
comprehensive
cellular
composition;
diversity
function;
spatial
architecture
such
as
zonation,
communication,
proximity;
identity
conversion
cell-specific
alterations
are
associated
with
pathology;
therapeutic
targets.
further
discuss
outstanding
challenges,
advanced
experimental
technologies,
computational
methods
help
to
address
challenges.
npj Imaging,
Journal Year:
2024,
Volume and Issue:
2(1)
Published: July 17, 2024
Abstract
The
recent
upswing
in
the
integration
of
spatial
multi-omics
for
conducting
multidimensional
information
measurements
is
opening
a
new
chapter
biological
research.
Mapping
landscape
various
biomolecules
including
metabolites,
proteins,
nucleic
acids,
etc.,
and
even
deciphering
their
functional
interactions
pathways
believed
to
provide
more
holistic
nuanced
exploration
molecular
intricacies
within
living
systems.
Mass
spectrometry
imaging
(MSI)
stands
as
forefront
technique
spatially
mapping
metabolome,
lipidome,
proteome
diverse
tissue
cell
samples.
In
this
review,
we
offer
systematic
survey
delineating
different
MSI
techniques
resolved
analysis,
elucidating
principles,
capabilities,
limitations.
Particularly,
focus
on
advancements
methodologies
aimed
at
augmenting
sensitivity
specificity
MSI;
depict
burgeoning
MSI-based
metabolomics,
lipidomics,
proteomics,
encompassing
synergy
with
other
modalities.
Furthermore,
speculative
insights
into
potential
trajectory
technology
future.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(4), P. 2249 - 2249
Published: Feb. 13, 2024
Lipids
represent
a
large
group
of
biomolecules
that
are
responsible
for
various
functions
in
organisms.
Diseases
such
as
diabetes,
chronic
inflammation,
neurological
disorders,
or
neurodegenerative
and
cardiovascular
diseases
can
be
caused
by
lipid
imbalance.
Due
to
the
different
stereochemical
properties
composition
fatty
acyl
groups
molecules
most
classes,
quantification
lipids
development
lipidomic
analytical
techniques
problematic.
Identification
species
from
complex
matrices
is
difficult,
therefore
individual
steps,
which
include
extraction,
separation,
detection
lipids,
must
chosen
properly.
This
review
critically
documents
recent
strategies
analysis
sample
pretreatment
instrumental
data
interpretation
published
last
five
years
(2019
2023).
The
advantages
disadvantages
extraction
methods
covered.
step
comprises
identification
quantification.
Mass
spectrometry
(MS)
used
technique
analysis,
performed
direct
infusion
MS
approach
combination
with
suitable
separation
liquid
chromatography
gas
chromatography.
Special
attention
also
given
correct
evaluation
obtained
analyses.
Only
accurate,
precise,
robust
reliable
able
bring
useful
information,
may
contribute
clarification
some
at
molecular
level,
putative
biomarkers
and/or
therapeutic
targets.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: May 19, 2023
Abstract
Tumors
are
comprised
of
a
multitude
cell
types
spanning
different
microenvironments.
Mass
spectrometry
imaging
(MSI)
has
the
potential
to
identify
metabolic
patterns
within
tumor
ecosystem
and
surrounding
tissues,
but
conventional
workflows
have
not
yet
fully
integrated
breadth
experimental
techniques
in
metabolomics.
Here,
we
combine
MSI,
stable
isotope
labeling,
spatial
variant
Isotopologue
Spectral
Analysis
map
distributions
metabolite
abundances,
nutrient
contributions,
turnover
fluxes
across
brains
mice
harboring
GL261
glioma,
widely
used
model
for
glioblastoma.
When
with
combination
ion
mobility,
desorption
electrospray
ionization,
matrix
assisted
laser
ionization
reveals
alterations
multiple
anabolic
pathways.
De
novo
fatty
acid
synthesis
flux
is
increased
by
approximately
3-fold
glioma
relative
healthy
tissue.
Fatty
elongation
elevated
even
higher
at
8-fold
tissue
highlights
importance
elongase
activity
glioma.
Chemical Communications,
Journal Year:
2024,
Volume and Issue:
60(16), P. 2137 - 2151
Published: Jan. 1, 2024
In
this
feature
article,
we
discuss
quantitative
mass
spectrometry
imaging
of
therapeutics
and
biomolecules
conducted
by
recent
studies
for
matrix-assisted
laser
desorption/ionization
(MALDI)
desorption
electrospray
ionization
(DESI)
techniques.
Chinese Medicine,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: March 13, 2024
Abstract
As
an
important
part
of
medical
science,
Traditional
Chinese
Medicine
(TCM)
attracts
much
public
attention
due
to
its
multi-target
and
multi-pathway
characteristics
in
treating
diseases.
However,
the
limitations
traditional
research
methods
pose
a
dilemma
for
evaluation
clinical
efficacy,
discovery
active
ingredients
elucidation
mechanism
action.
Therefore,
innovative
approaches
that
are
line
with
TCM
theory
practice
urgently
needed.
Chinmendomics,
newly
emerging
strategy
evaluating
efficacy
TCM,
is
proposed.
This
combines
systems
biology,
serum
pharmacochemistry
bioinformatics
evaluate
holistic
view
by
accurately
identifying
syndrome
biomarkers
monitoring
their
complex
metabolic
processes
intervened
finding
agents
associated
course
pharmacodynamic
constructing
bioinformatics-based
correlation
network
model
further
reveal
interaction
between
targets.
In
this
article,
we
review
recent
progress
Chinmedomics
promote
application
modernisation
internationalisation
TCM.
Current Opinion in Biotechnology,
Journal Year:
2024,
Volume and Issue:
86, P. 103068 - 103068
Published: Feb. 7, 2024
Profiling
spatial
distributions
of
lipids,
metabolites,
and
proteins
in
tumors
can
reveal
unique
cellular
microenvironments
provide
molecular
evidence
for
cancer
cell
dysfunction
proliferation.
Mass
spectrometry
imaging
(MSI)
is
a
label-free
technique
that
be
used
to
map
biomolecules
situ.
Here,
we
discuss
current
progress
applying
MSI
uncover
heterogeneity
tumors.
First,
the
analytical
strategies
profile
small
molecules
are
outlined,
methods
multimodal
maximize
biological
information
highlighted.
Second,
present
summarize
insights
obtained
by
tumor
tissue.
Finally,
important
considerations
designing
experiments
several
challenges.
