Aging,
Journal Year:
2024,
Volume and Issue:
16(13), P. 10943 - 10971
Published: June 26, 2024
The
genomic
landscape
of
clear
cell
renal
carcinoma
(ccRCC)
has
a
considerable
intra-tumor
heterogeneity,
which
is
significant
obstacle
in
the
field
precision
oncology
and
plays
pivotal
role
metastasis,
recurrence,
therapeutic
resistance
cancer.
mechanisms
heterogeneity
ccRCC
have
yet
to
be
fully
established.
We
integrated
single-cell
RNA
sequencing
(scRNA-seq)
transposase-accessible
chromatin
(scATAC-seq)
data
from
multi-omics
perspective.
Based
on
consensus
non-negative
matrix
factorization
(cNMF)
algorithm,
functionally
heterogeneous
cancer
cells
were
classified
into
metabolism,
inflammatory,
EMT
meta
programs,
with
spatial
transcriptomics
(stRNA-seq)
providing
information
such
disparate
programs
cells.
bulk
(RNA-seq)
revealed
high
clinical
prognostic
values
three
transcription
factor
regulatory
network
motif
activities
revealing
key
factors
that
regulate
interactions
between
varying
other
subpopulations
microenvironment
investigated.
Finally,
we
assessed
sensitivity
different
anti-cancer
agents.
Our
findings
inform
its
networks
offers
new
perspectives
facilitate
designs
rational
strategies.
Cancer Reports,
Journal Year:
2023,
Volume and Issue:
6(10)
Published: Sept. 9, 2023
Patients
with
combined
hepatocellular-cholangiocarcinoma
(cHCC-CCA)
have
limited
treatment
options
and
poor
prognosis.
Tumor-associated
macrophages
(TAMs)
are
the
most
abundant
infiltrating
immune
cells
in
tumor
microenvironment
promote
stemness,
proliferation,
invasion
metastasis.
Evidence
suggested
that
transthyretin
(TTR)
influenced
prolifetation
functions
of
different
tumors
play
an
essential
role
microenvironment.
Hepatoma Research,
Journal Year:
2023,
Volume and Issue:
unknown
Published: July 13, 2023
Cholangiocarcinoma
(CCA)
is
an
extremely
aggressive
malignancy
characterized
by
a
very
limited
prognosis
and
scarce
treatment
options.
The
majority
of
patients
are
diagnosed
at
advanced
stage
do
not
qualify
for
potentially
curative
surgical
treatments,
making
CCA
increasingly
prevalent
global
challenge.
highly
reactive
desmoplastic
stroma,
with
complex
mechanisms
underlying
the
mutual
interactions
between
tumor
cells
stromal
compartment.
This
review
focuses
on
recent
studies
examining
CCA’s
biological
features,
particular
reference
to
stroma
(TRS)
its
role
in
progression,
including
matrix
remodeling,
angiogenesis
lymphangiogenesis,
metastasis,
immune
evasion.
After
giving
panoramic
view
relationship
tumoral
compartment
(cancer-associated
fibroblast,
CAFs
tumor-associated
macrophages,
TAMs),
this
also
discusses
current
therapeutic
approaches
counteract
TAMs
effects
progression.
Clinical Hemorheology and Microcirculation,
Journal Year:
2023,
Volume and Issue:
86(4), P. 467 - 479
Published: Nov. 28, 2023
OBJECTIVE:
This
article
aims
to
clarify
pitfalls
and
find
strategies
for
the
detecting
diagnosing
hyperechoic
liver
metastases
(LMs)
using
Sonazoid-contrast
enhanced
ultrasonography
(Sonazoid-CEUS).
METHODS:
study
was
a
prospective
self-controlled
study.
Patients
with
hepatic
lesions
suspected
as
LMs
or
benign
were
included
in
Baseline
(BUS)
Sonazoid-CEUS
performed
on
every
patient.
Characteristics
of
nodules
compared
by
chi-square
test
fisher
test.
Factors
influenced
CEUS
demonstrated
univariate
analysis
multivariate
logistic
regression
analysis.
RESULTS:
54
patients
this
found
additional
75
from
19
Kupffer
phase.
We
focal
deep
seated
are
main
confounding
factors
diagnosis.
Sensitivity
would
be
improved
16.67%
78.57%,
negative
predictive
value
(NPV)
28.57%
76.92%
accuracy
37.5%
87.50%
when
rapid
“wash-in”
“wash-out”
diagnostic
criteria.
CONCLUSIONS:
Hyperechoic
especially
deeply
ones
usually
not
shown
typical
“black
hole”
sign
Quickly
“wash-in
wash
out”
shows
high
malignant
nodules.
highly
recommend
routing
exam
detect
diagnose
LMs.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 22, 2023
Spatial
transcriptomics
is
revolutionising
the
exploration
of
intratissue
heterogeneity
in
cancer,
yet
capturing
cellular
niches
and
their
spatial
relationships
remains
challenging.
We
introduce
SpottedPy,
a
Python
package
designed
to
identify
tumour
hotspots
map
interactions
within
cancer
ecosystem.
Using
we
examine
epithelial-mesenchymal
plasticity
breast
highlight
stable
associated
with
angiogenic
hypoxic
regions,
shielded
by
CAFs
macrophages.
Hybrid
mesenchymal
hotspot
distribution
followed
transformation
gradients
reflecting
progressive
immunosuppression.
Our
method
offers
flexibility
explore
at
different
scales,
from
immediate
neighbours
broader
tissue
modules,
providing
new
insights
into
microenvironment
dynamics.
Aging,
Journal Year:
2024,
Volume and Issue:
16(13), P. 10943 - 10971
Published: June 26, 2024
The
genomic
landscape
of
clear
cell
renal
carcinoma
(ccRCC)
has
a
considerable
intra-tumor
heterogeneity,
which
is
significant
obstacle
in
the
field
precision
oncology
and
plays
pivotal
role
metastasis,
recurrence,
therapeutic
resistance
cancer.
mechanisms
heterogeneity
ccRCC
have
yet
to
be
fully
established.
We
integrated
single-cell
RNA
sequencing
(scRNA-seq)
transposase-accessible
chromatin
(scATAC-seq)
data
from
multi-omics
perspective.
Based
on
consensus
non-negative
matrix
factorization
(cNMF)
algorithm,
functionally
heterogeneous
cancer
cells
were
classified
into
metabolism,
inflammatory,
EMT
meta
programs,
with
spatial
transcriptomics
(stRNA-seq)
providing
information
such
disparate
programs
cells.
bulk
(RNA-seq)
revealed
high
clinical
prognostic
values
three
transcription
factor
regulatory
network
motif
activities
revealing
key
factors
that
regulate
interactions
between
varying
other
subpopulations
microenvironment
investigated.
Finally,
we
assessed
sensitivity
different
anti-cancer
agents.
Our
findings
inform
its
networks
offers
new
perspectives
facilitate
designs
rational
strategies.
