Biomolecular fingerprints of the effect of zoledronic acid on prostate cancer stem cells: Comparison of 2D and 3D cell culture models

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 753, P. 109920 - 109920

Published: Feb. 1, 2024

Language: Английский

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Hypoxia promotes conversion to a stem cell phenotype in prostate cancer cells by activating HIF-1α/Notch1 signaling pathway

Clinical & Translational Oncology, Journal Year: 2023, Volume and Issue: 25(7), P. 2138 - 2152

Published: Feb. 9, 2023

Language: Английский

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Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 7, 2023

Abstract Background Prostate cancer (PCA) is the fifth leading cause of cancer-related deaths worldwide, with limited treatment options in advanced stages. The immunosuppressive tumor microenvironment (TME) PCA results lower sensitivity to immunotherapy. Although molecular subtyping expected offer important clues for precision PCA, there currently a shortage dependable and effective typing methods available clinical practice. Therefore, we aim propose novel stemness-based classification approach guide personalized treatments, including Methods An integrative multi-omics analysis was performed evaluate stemness-level heterogeneities. Unsupervised hierarchical clustering used classify PCAs based on stemness signature genes. To make patient more clinically applicable, subtype predictor jointly developed by using four datasets 76 machine learning algorithms. Results We identified signatures comprising 18 signaling pathways, which classified samples into three subtypes via unsupervised clustering: low (LS), medium (MS), high (HS) subtypes. HS patients are sensitive androgen deprivation therapy, taxanes, immunotherapy have highest stemness, malignancy, mutation load (TMB) levels, worst prognosis, immunosuppression. LS platinum-based chemotherapy but resistant lowest TMB best immune infiltration. MS represent an intermediate status levels moderate prognosis. further demonstrated that these conserved across pan-tumor. Additionally, 9-gene has comparable capability pathways diagnosis predict recurrence, metastasis, progression, efficacy different treatments. Conclusions potential be powerful tool pan-cancer, selection or other treatments patients.

Language: Английский

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Prostate cancer stem cells and their targeted therapies

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 8, 2024

Prostate cancer (PCa) is the most common malignancy among men worldwide. Through androgen receptor signaling inhibitor (ARSI) treatment, patients eventually succumb to castration-resistant prostate (CRPC). For this, stem cells (PCSCs), as a minor population of tumor that can promote relapse, ARSI resistance, and disease progression, are gaining attention. Therefore, specific therapy targeting PCSCs has momentum. This study reviewed identification characterization PCSC-based putative biomarkers summarized their mechanisms action. We further discussed clinical trials novel therapeutic interventions focused on PCSC-related pathways, PCSC microenvironment, cutting-edge miRNA therapy, immunotherapy approaches from mechanistic standpoint. review provides updated insights into plasticity, identifying new optimized treatments for with advanced PCa.

Language: Английский

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Nuclear receptor TLX functions to promote cancer stemness and EMT in prostate cancer via its direct transactivation of CD44 and stem cell-regulatory transcription factors

British Journal of Cancer, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Language: Английский

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Embryonic microenvironment suppresses YY1 and YY1-related genes in prostate cancer stem cells

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155467 - 155467

Published: July 15, 2024

Language: Английский

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Application and new findings of scRNA-seq and ST-seq in prostate cancer

Cell Regeneration, Journal Year: 2024, Volume and Issue: 13(1)

Published: Oct. 29, 2024

Abstract Prostate cancer is a malignant tumor of the male urological system with highest incidence rate in world, which seriously threatens life and health middle-aged elderly men. The progression prostate involves interaction between cells microenvironment. Understanding mechanisms pathogenesis disease important to guide diagnosis therapy. emergence single-cell RNA sequencing (scRNA-seq) spatial transcriptome (ST-seq) technologies has brought breakthroughs study cancer. It makes up for defects traditional techniques such as fluorescence-activated cell sorting that are difficult elucidate cell-specific gene expression. This review summarized heterogeneity functional changes microenvironment revealed by scRNA-seq ST-seq, aims provide reference optimal treatment

Language: Английский

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Doublecortin-like kinase 1 promotes stem cell-like properties through the Hippo-YAP pathway in prostate cancer

International Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 22(2), P. 460 - 472

Published: Dec. 31, 2024

Background: Doublecortin-like kinase 1 (DCLK1) has been revealed to be involved in modulating cancer stemness and tumor progression, but its role prostate (PCa) remains obscure.Castration-resistant metastatic PCa exhibit aggressive behaviors, current therapeutic approaches have shown limited beneficial effects on the overall survival rate of patients with advanced PCa.This study aimed investigate biological potential molecular mechanism DCLK1 progression PCa.Methods: The maintaining stem cell-like properties was explored via gain-and loss-of-function studies, including colony formation assays, sphere assays measurement stemness-related marker expression.A set transcriptomic data for downloaded from Cancer Genome Atlas analyze correlations between Hippo pathway gene expression.The by which regulates signaling further investigated vitro methods such as Western blot analysis, quantitative real-time PCR immunofluorescence staining, luciferase reporter vivo animal studies.Results: studies demonstrated that upregulating promoted downregulating suppressed aspects phenotype, formation, expression markers (c-Myc, OCT4, CD44, NANOG, SOX2, KLF4).Importantly, bioinformatics analysis indicated is closely correlated PCa.Further inhibits pathway, leading yes-associated protein (YAP) activation large suppressor homolog (LATS1).Moreover, effect abolishing traits observed after treatment verteporfin, a small molecule inhibitor YAP.Consistent findings, findings confirmed tumorigenicity cells Hippo-YAP signaling.Conclusion: promotes characteristics inducing LATS1-mediated YAP activation, ultimately growth progression.Thus, our identify an attractive candidate development cell-targeted therapies improve outcomes PCa.

Language: Английский

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Hypothetical involvement of stress hormones-induced reprograming of adult stem/progenitor cells in tumorigenesis

Published: July 15, 2024

Stress is a state of threatened or perceived as homeostasis that can be induced by various external and internal stimuli such psychosocial factors, inflammatory injurious conditions, infections. In order to restore body homeostasis, adrenal glands produce secrete glucocorticoids (GCs) catecholamines (CAs), which are the main stress hormones support survival adaptation organisms new environment. contrast rather beneficial impact acute short-lasting stress, chronic related dysregulation system implicated in development many non-communicable diseases, including cancer. Particularly, ever-increasing experimental clinical evidence implicates involvement CAs GCs well overexpression their receptors activation major pathways involved tumour development, metastasis, resistance therapies. More importantly, results epidemiological studies revealed overexposure during pre- early postnatal life might induce life-long even transgenerational predispose it tumours. Although exact mechanisms latter process not yet fully known, has been demonstrated GC-induced epigenetic modifications change expression several key genes regulation system, initiation, imprinting. When alterations occur stem/progenitor cells (SPCs), this only lead long-term dysfunction but promote generation cancer stem (CSCs). This review article discusses hypothesis hormones-mediated reprograming SPCs sensitive developmental periods, contribute increased sensitivity malignant transformation, thereby promoting tumorigenesis.

Language: Английский

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Tumor Microenvironment and Dermatological Conditions in Prostate Cancer

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(21), P. 9655 - 9655

Published: Oct. 22, 2024

Tumor initiation, progression, and invasion are closely related to the tumor microenvironment. Inflammation can modulate activity of cells in TME contribute all stages development. The etiopathogenesis cutaneous manifestations associated with prostate cancer is unclear. phenotype could be supported by intratumoral heterogeneity, remodeling interactions microenvironment, dynamics epithelial–mesenchymal transition. Among urinary system cancers, presents few signs symptoms, most being diagnosed advanced disease. In this review, we analyze events cancer, represented direct or indirect primary malignancy skin toxicities caused oncological medications.

Language: Английский

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