circRNA6448-14/miR-455-3p/OTUB2 axis stimulates glycolysis and stemness of esophageal squamous cell carcinoma DOI Creative Commons
Yaowen Zhang, Heming Zhang, Chenyu Wang

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(11), P. 9485 - 9497

Published: May 30, 2024

Background: Esophageal squamous cell carcinoma (ESCC) is a gastrointestinal malignancy with high incidence. This study aimed to reveal the complete circRNA-miRNA-mRNA regulatory network in ESCC and validate its function mechanism. Method: Expression of OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 2 (OTUB2) was analyzed by bioinformatics find binding sites between circRNA6448-14 miR-455-3p, as well miR-455-3p OTUB2. The relationships were verified RNA Immunoprecipitation (RIP) dual-luciferase assay. expressions circRNA6448-14, OTUB2 detected quantitative real-time polymerase chain reaction (qRT-PCR). MTT assay measured viability, spheroid formation assessed ability stem sphere formation. Western blot (WB) determined expression marker proteins surface rate-limiting enzyme glycolysis. Seahorse XFe96 extracellular flux analyzer rate acidification cellular oxygen consumption. Corresponding kits glucose consumption, lactate production, adenosine triphosphate (ATP) generation. Results: In ESCC, highly expressed contrast miR-455-3p. Knocking down could prevent glycolysis stemness cells. Additionally, enhanced sponging Overexpression or silencing reversed inhibitory effect knockdown on stemness. Conclusion: research demonstrated that circRNA6448-14/miR-455-3p/OTUB2 axis induced Our revealed novel which may serve potential therapeutic target for ESCC.

Language: Английский

Non-coding RNAs in thoracic disease: Barrett’s esophagus and esophageal adenocarcinoma DOI

Siyuan Sheng,

Jianhui Guo,

Chuangang Lu

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120242 - 120242

Published: March 1, 2025

Language: Английский

Citations

0
CircMMP11 is a Potential Recurrence Biomarker and Facilitates Progression of Esophageal Squamous Cell Carcinoma DOI
Jingnan Yuan, Ying Cui, Jing Zhang

et al.

Biochemical Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Language: Английский

Citations

0
Circular RNAs in hepatitis B virus-induced hepatocellular carcinoma: A comprehensive review and recent advances DOI Creative Commons

Wenying Quan,

Kizito Eneye Bello,

Rafidah Hanim Shueb Shomiad

et al.

Genes & Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 101605 - 101605

Published: March 1, 2025

Language: Английский

Citations

0
circRNA6448-14/miR-455-3p/OTUB2 axis stimulates glycolysis and stemness of esophageal squamous cell carcinoma DOI Creative Commons
Yaowen Zhang, Heming Zhang, Chenyu Wang

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(11), P. 9485 - 9497

Published: May 30, 2024

Background: Esophageal squamous cell carcinoma (ESCC) is a gastrointestinal malignancy with high incidence. This study aimed to reveal the complete circRNA-miRNA-mRNA regulatory network in ESCC and validate its function mechanism. Method: Expression of OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 2 (OTUB2) was analyzed by bioinformatics find binding sites between circRNA6448-14 miR-455-3p, as well miR-455-3p OTUB2. The relationships were verified RNA Immunoprecipitation (RIP) dual-luciferase assay. expressions circRNA6448-14, OTUB2 detected quantitative real-time polymerase chain reaction (qRT-PCR). MTT assay measured viability, spheroid formation assessed ability stem sphere formation. Western blot (WB) determined expression marker proteins surface rate-limiting enzyme glycolysis. Seahorse XFe96 extracellular flux analyzer rate acidification cellular oxygen consumption. Corresponding kits glucose consumption, lactate production, adenosine triphosphate (ATP) generation. Results: In ESCC, highly expressed contrast miR-455-3p. Knocking down could prevent glycolysis stemness cells. Additionally, enhanced sponging Overexpression or silencing reversed inhibitory effect knockdown on stemness. Conclusion: research demonstrated that circRNA6448-14/miR-455-3p/OTUB2 axis induced Our revealed novel which may serve potential therapeutic target for ESCC.

Language: Английский

Citations

1