Bioinformatics and next generation data analysis reveals the potential role of inflammation in sepsis and its associated complications DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 5, 2023

Abstract Sepsis is the leading systemic inflammatory response syndrome in worldwide, yet relatively little known about genes and signaling pathways involved sepsis progression. The current investigation aimed to elucidate potential key candidate its associated complications. Next generation sequencing (NGS) dataset (GSE185263) was downloaded from Gene Expression Omnibus (GEO) database, which included data 348 samples 44 normal control samples. Differentially expressed (DEGs) were identified using t-tests DESeq2 R package. Next, we made use of g:Profiler analyze gene ontology (GO) REACTOME pathway. Then protein-protein interaction (PPI) these DEGs visualized by Cytoscape with Search Tool for Retrieval Interacting Genes (STRING). Furthermore, constructed miRNA-hub regulatory network TF-hub among hub utilizing miRNet NetworkAnalyst online databases tool software. Finally, performed receiver operating characteristic (ROC) curve analysis through pROC package statistical In total, 958 identified, 479 up regulated down regulated. GO results showed that mainly enriched regulation cellular process, stimulus, extracellular matrix organization immune system. PRKN, KIT, FGFR2, GATA3, ERBB3, CDK1, PPARG, H2BC5, H4C4 CDC20 might be Predicted miRNAs (e.g., hsa-mir-548ad-5p hsa-mir-2113) TFs YAP1 TBX5) found significantly correlated conclusion, DEGs, relative pathways, genes, miRNA help understanding molecular mechanisms underlying complications progression provide targets biomarkers

Language: Английский

The HSP90AB1-mediated upregulation of IDO1 can promote the progression of colorectal cancer DOI Creative Commons
Chenchen Jin, Xuejiao Xu, Tao Li

et al.

ONCOLOGIE, Journal Year: 2024, Volume and Issue: 26(3), P. 445 - 455

Published: April 4, 2024

Abstract Objectives Colorectal cancer (CRC) is a global challenge, and heat shock protein 90 (HSP90) identified as key driver in progression. However, the tumor-promoting mechanism of HSP90 CRC, particularly HSP90AB1, remains unclear. This study aims to explore analyze oncogenic HSP90AB1 CRC identify potential therapeutic targets. Materials methods expression underwent analysis cell lines tissues at mRNA levels. Through use shRNA, targeted suppression was achieved lines, enabling its influence on proliferation, invasion, apoptosis, cycle Subsequent investigation focused elucidating regulatory relationship between IDO1, employing combination bioinformatics approaches vitro /vivo experiments. These efforts confirmed IDO1 downstream target provided insight into role driving Results exhibits overexpression both tumor (p<0.05). Its downregulation impedes proliferation invasion (p<0.01), promotes apoptosis arrest Investigation reveals that decreased leads inhibition suggesting regulation plays crucial mediating pro-tumorigenic effects HSP90AB1. In vivo experiments confirm substantial reduction growth upon knockdown xenograft models (p<0.01). this tumor-suppressive effect reversed highlighting Conclusions exerts progression by upregulating IDO1.

Language: Английский

Citations

0
Chemo-Immunotherapy by Nanoliposomal Epacadostat and Docetaxel Combination to Ido1 Inhibition and Tumor Microenvironment Suppression DOI

Rahimeh Khoshkhabar,

Mona Yazdani,

Seyedeh Hoda Alavizadeha

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Construction and validation of a neovascular glaucoma nomogram in patients with diabetic retinopathy after pars plana vitrectomy DOI Creative Commons
Yi Shi,

Yan-Xin Zhang,

Mingfei Jiao

et al.

World Journal of Diabetes, Journal Year: 2024, Volume and Issue: 15(4), P. 654 - 663

Published: April 11, 2024

Neovascular glaucoma (NVG) is likely to occur after pars plana vitrectomy (PPV) for diabetic retinopathy (DR) in some patients, thus reducing the expected benefit. Understanding risk factors NVG occurrence and building effective prediction models are currently required clinical research.

Language: Английский

Citations

0
Clinicopathological and Prognostic Significance of Indoleamine 2,3-Dioxygenase (IDO) Expression in Head and Neck Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis DOI

Hanan M. Qasem,

Ramez M. Odat, Sakhr Alshwayyat

et al.

Journal of Stomatology Oral and Maxillofacial Surgery, Journal Year: 2024, Volume and Issue: unknown, P. 102130 - 102130

Published: Oct. 1, 2024

Language: Английский

Citations

0
KRas plays a negative role in regulating IDO1 expression DOI

Xiandong Peng,

Eun‐Ji Lee,

Ji‐An Liang

et al.

Translational Oncology, Journal Year: 2024, Volume and Issue: 51, P. 102167 - 102167

Published: Nov. 16, 2024

Language: Английский

Citations

0
Bioinformatics and next generation data analysis reveals the potential role of inflammation in sepsis and its associated complications DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 5, 2023

Abstract Sepsis is the leading systemic inflammatory response syndrome in worldwide, yet relatively little known about genes and signaling pathways involved sepsis progression. The current investigation aimed to elucidate potential key candidate its associated complications. Next generation sequencing (NGS) dataset (GSE185263) was downloaded from Gene Expression Omnibus (GEO) database, which included data 348 samples 44 normal control samples. Differentially expressed (DEGs) were identified using t-tests DESeq2 R package. Next, we made use of g:Profiler analyze gene ontology (GO) REACTOME pathway. Then protein-protein interaction (PPI) these DEGs visualized by Cytoscape with Search Tool for Retrieval Interacting Genes (STRING). Furthermore, constructed miRNA-hub regulatory network TF-hub among hub utilizing miRNet NetworkAnalyst online databases tool software. Finally, performed receiver operating characteristic (ROC) curve analysis through pROC package statistical In total, 958 identified, 479 up regulated down regulated. GO results showed that mainly enriched regulation cellular process, stimulus, extracellular matrix organization immune system. PRKN, KIT, FGFR2, GATA3, ERBB3, CDK1, PPARG, H2BC5, H4C4 CDC20 might be Predicted miRNAs (e.g., hsa-mir-548ad-5p hsa-mir-2113) TFs YAP1 TBX5) found significantly correlated conclusion, DEGs, relative pathways, genes, miRNA help understanding molecular mechanisms underlying complications progression provide targets biomarkers

Language: Английский

Citations

0