The ability of clostridium novyi-NT spores to induce apoptosis via the mitochondrial pathway in mice with HPV-positive cervical cancer tumors derived from the TC-1 cell line DOI Creative Commons

Behrouz Ebadi Sharafabad,

Asghar Abdoli,

Parisa Jamour

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

A precise observation is that the cervix's solid tumors possess hypoxic regions where oxygen concentration drops below 1.5%. Hypoxia negatively impacts host's immune system and significantly diminishes effectiveness of several treatments, including radiotherapy chemotherapy. Utilizing oncolytic spores Clostridium novyi-NT to target has emerged as a noteworthy treatment strategy. The transplantation procedure involved injecting TC-1 cells, capable expressing HPV-16 E6/7 oncoproteins, into subcutaneous layer 6-8-week-old female C57/BL6 mice. cell line, was subcutaneously transplanted tumor-bearing mice were randomly divided 4 groups, after selecting control group, they treated with different methods. Group 1- without (0.1 ml sterile PBS intratumor) 2- received cisplatin intraperitoneally (10 mg/kg) 3- 107Clostridium systemically through tail vein 4-tumor intratumorally. 20 days start treatment, sacrificed tumor tissues isolated. In order clarify mechanism therapeutic effect spores, amount ROS ceramide measured by ELISA technique, expression level cytochrome c, cleaved caspase- 3, Bax, Bcl-2, HIF-1α, VEGF proteins western blotting. Our results clearly showed injection (either intratumorally or intravenously) causes regression mouse cervical tumors. Spore germination induces internal apoptosis in cancer cells inducing production increasing total ceramide, releasing c damaging mitochondria. Additionally, provided clear evidence significant decrease HIF-1 alpha among groups when compared both cisplatin-treated group group. study's outcomes demonstrated introduction triggered (derived from line) via mitochondrial pathway, subsequently resulting model.

Language: Английский

Reactive oxygen species induced by SARS-CoV-2 infection can induce EMT in solid tumors: potential role of COVID-19 in chemo-resistance and metastasis DOI Creative Commons

Melina Moulaeian,

Saeid Ferdousmakan,

Sara Banihashemi

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(22), P. e40297 - e40297

Published: Nov. 1, 2024

The coronavirus disease 2019 (COVID-19) pandemic has raised discussion over the connection between viral infections and biology of cancer. Research investigated relationship signaling pathways stimulated by severe acute respiratory syndrome 2 (SARS-CoV-2) that may be involved in progression cancer, resistance to chemotherapy, metastasis. However, exact cellular molecular mechanisms effects SARS-CoV-2 infection on cancer progression, chemo-resistance, metastasis, recurrence have not been fully understood. Recently, studies indicate might induce inflammatory responses cytokine storm, which can affect associated with epithelial-mesenchymal transition (EMT). We address possible involvement reactive oxygen species (ROS) induced treatment resistance, metastatic recurrence, activation EMT solid tumors this review. emphasize disturbance mitochondria dysfunction, overproduction ROS SARS-CoV-2-infected cells, its consequences for beginning EMT. also suggested processes influence EMT, pathways, interaction mitochondria. Gaining knowledge about ROS's function SARS CoV-2 condition, promoting will help develop effective strategies during therapy treatments lowering drug patient.

Language: Английский

Citations

1
Affinity of PET-MRI Tracers for Hypoxic Cells in Breast Cancer: A Systematic Review DOI Creative Commons
Ioana-Claudia Costin, Loredana G. Marcu

Cells, Journal Year: 2024, Volume and Issue: 13(12), P. 1048 - 1048

Published: June 17, 2024

Tumour hypoxia is a known microenvironmental culprit for treatment resistance, tumour recurrence and promotion of metastatic spread. Despite the long-known existence this factor within milieu, still one greatest challenges in cancer management. The transition from invasive less reliable detection methods to more accurate non-invasive ways identify quantify was long process that eventually led promising results showed by functional imaging techniques. Hybrid imaging, such as PET-CT, has great advantage combining structural or anatomical image (offered CT) with metabolic PET). However, context hypoxia, it only PET taken after appropriate radiotracer administration would supply hypoxia-specific information. To overcome limitation, development latest hybrid systems, PET-MRI, enables synergistic approach towards both having potential provide information on microenvironment. This study designed systematic review literature newest developments PET-MRI hypoxic cells breast cancer. analysis includes affinity various tracers patient group well correlations between PET-specific MRI-specific parameters, offer broader view widespread clinical implementation technique.

Language: Английский

Citations

0
Lung bronchiectasisas a paradigm of the interplay between infection and colonization on plastic modulation of the pre-metastatic niche DOI Creative Commons

Lucrezia Pisanu,

Klodjana Mucaj,

Valentina Conio

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 14, 2024

The lungs are most often a preferential target organ for malignant spreading and growth. It is well known that chronic parenchymal inflammation prolonged injuries represents an independent risk factor cancer onset. Growing evidence supports the implication of lung microbiota in pathogenesis cancer. However, full interplay between inflammation, bacterial colonization, pathologic condition as bronchiectasis growth deserves better clarification. We here aim at presenting analyzing original data discussing state-of-the-art on knowledge regarding how this complex milieu acts plasticity pre-metastatic niche to point out rationale early diagnosis therapeutic targeting.

Language: Английский

Citations

0
The ability of clostridium novyi-NT spores to induce apoptosis via the mitochondrial pathway in mice with HPV-positive cervical cancer tumors derived from the TC-1 cell line DOI Creative Commons

Behrouz Ebadi Sharafabad,

Asghar Abdoli,

Parisa Jamour

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

A precise observation is that the cervix's solid tumors possess hypoxic regions where oxygen concentration drops below 1.5%. Hypoxia negatively impacts host's immune system and significantly diminishes effectiveness of several treatments, including radiotherapy chemotherapy. Utilizing oncolytic spores Clostridium novyi-NT to target has emerged as a noteworthy treatment strategy. The transplantation procedure involved injecting TC-1 cells, capable expressing HPV-16 E6/7 oncoproteins, into subcutaneous layer 6-8-week-old female C57/BL6 mice. cell line, was subcutaneously transplanted tumor-bearing mice were randomly divided 4 groups, after selecting control group, they treated with different methods. Group 1- without (0.1 ml sterile PBS intratumor) 2- received cisplatin intraperitoneally (10 mg/kg) 3- 107Clostridium systemically through tail vein 4-tumor intratumorally. 20 days start treatment, sacrificed tumor tissues isolated. In order clarify mechanism therapeutic effect spores, amount ROS ceramide measured by ELISA technique, expression level cytochrome c, cleaved caspase- 3, Bax, Bcl-2, HIF-1α, VEGF proteins western blotting. Our results clearly showed injection (either intratumorally or intravenously) causes regression mouse cervical tumors. Spore germination induces internal apoptosis in cancer cells inducing production increasing total ceramide, releasing c damaging mitochondria. Additionally, provided clear evidence significant decrease HIF-1 alpha among groups when compared both cisplatin-treated group group. study's outcomes demonstrated introduction triggered (derived from line) via mitochondrial pathway, subsequently resulting model.

Language: Английский

Citations

0