Unjamming Transition as a Paradigm for Biomechanical Control of Cancer Metastasis DOI Creative Commons
Grace Cai, Nicole C. Rodgers, Allen P. Liu

et al.

Cytoskeleton, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

ABSTRACT Tumor metastasis is a complex phenomenon that poses significant challenges to current cancer therapeutics. While the biochemical signaling involved in promoting motile phenotypes well understood, role of biomechanical interactions has recently begun be incorporated into models tumor cell migration. Specifically, we propose unjamming transition, adapted from physical paradigms describing behavior granular materials, better discern transition toward an invasive phenotype. In this review, introduce jamming broadly and narrow our discussion different modes 3D migration arise. Then discuss mechanical between cells their neighbors, along with surrounding extracellular matrix. We center on induce state or these contexts. By considering interplay migration, can advance understanding control metastasis.

Language: Английский

Estrogen receptors and extracellular matrix: the critical interplay in cancer development and progression DOI Creative Commons

Sylvia Mangani,

Zoi Piperigkou,

Nikolaos E. Koletsis

et al.

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Cancer remains a significant global health concern. Breast cancer is multifaceted and prevalent disease influenced by several factors, among which estrogen receptors (ERs) the extracellular matrix (ECM) play pivotal roles. ERs, encompassing ERα ERβ, exert diversity on tumor behavior, cell signaling, invasion, metastatic potential, thus guiding breast prognosis. Understanding multifunctional connections between ERs ECM that mediate dynamics of microenvironment vital for unraveling complexity pathobiology identifying novel therapeutic targets. This critical review delves into intricate nature emphasizing their structural isoforms consequential impact outcomes. A detailed examination ER‐mediated signaling pathways reveals how differential expression ERβ influence behavior. The functional ERs‐matrix interactions emerge as factor in modulating epigenetic mechanisms cells, orchestrating changes cellular phenotype patterns modulators. Specifically, are shown to regulate cascades, while effects components activity highlight bidirectional regulatory axis. also highlighted, illustrating distinct contribution ECM‐mediated responses. underscores complex interplay ERα/β ECM, shedding light onto potential strategies targeting these improve management.

Language: Английский

Citations

7
Design and Characterization of an Optical Phantom for Mesoscopic Multimodal Fluorescence Lifetime Imaging and Optical Coherence Elastography DOI Creative Commons
Luis Chavez, Shan Gao, Vikas Pandey

et al.

Biomedical Optics Express, Journal Year: 2025, Volume and Issue: 16(3), P. 1006 - 1006

Published: Feb. 5, 2025

We developed a novel methodology for manufacturing multimodal, tissue-mimicking phantoms that exhibit both molecular and biomechanical contrast. This leverages the immiscibility of silicone hydrogels to create solid mesoscale with localized regions precisely controlled fluorescence, including fluorescence lifetime properties, adjustable stiffness, without requiring physical barriers. Mechanical, fluorescent, optical characterization confirmed tunability across range values relevant biomedical applications. A macroscale 3D phantom was fabricated, its properties were validated through imaging (FLI) coherence elastography (OCE). Validation demonstrated successful tuning mechanical contrasts within structure, highlighting feasibility multimodal FLI-OCE. new process is expected support development validation approaches study tumor microenvironment (TME), as well their impact on therapeutic efficacy, enhance targeted therapies.

Language: Английский

Citations

0
Mast Cells as a Factor in Regulation of Breast Cancer Stromal Component Associated with Breast Cancer Aggressiveness DOI Creative Commons
Oleksandr Mushii, Anna Pavlova,

V. BAZAS

et al.

Experimental Oncology, Journal Year: 2025, Volume and Issue: 46(4), P. 311 - 323

Published: Feb. 20, 2025

It has been proven that changes in the morphology, representation, and organization of collagen fibers contribute to formation a unique microenvironment, which is associated with metastatic potential malignant neoplasms due initiation cell migration polarization. Among modulators stroma, fibroblasts remain most widely studied today. At same time, much less attention focused on study immune cells tumor particular, mast (MCs). To investigate relationship between MCs status features matrix breast cancer (BCa). The was conducted postoperative material 78 patients BCa stage I-II. were assessed by histochemical method using toluidine blue. For estimation functional activity MCs, degranulation index calculated. COL1A1, COL3A1, MMP-9 expression tissue immunohistochemically. A visualization performed staining Malory. Microphotographs pre-processed Adobe Photoshop SS 2019 analyzed software packages CurveAlign v. 4.0 ImageJ. Tumor high density characterized an increased COL1A1 (p < 0.05), COL3A1 0.05). In lower index, become thicker shorter denser existence levels miR-155-5p (r = 0.703, p 0.009), 0.603, 0.043), 0.562, 0.039) stroma 0.546, 0.038), as well associations this miRNA fiber length -0.632, 0.013), width -0.522, 0.048), 0.699, 0.014) found. Significantly higher rates 0.05) recorded degranulation. During progression, role manifestation development increases. growing number infiltrated contributes activation MMP fibrillar expression. These lead remodeling directly reflected spatial matrix. proteases causes decrease fibrils, explained mature their disorganization three-dimensional space. obtained data allow us assert play key not only specific microenvironment but also determining direction promotes aggressiveness.

Language: Английский

Citations

0
Emerging views of biomechanics via embedded sensors in model tissues: pathways to the clinic DOI Creative Commons

Alejandro Forigua,

Benjamin E. Campbell,

Christopher Moraes

et al.

Current Opinion in Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 100587 - 100587

Published: March 1, 2025

Language: Английский

Citations

0
Unveiling Matrix Metalloproteinase 13’s Dynamic Role in Breast Cancer: A Link to Physical Changes and Prognostic Modulation DOI Open Access
Xiaomeng Sun, Xiaojuan Hu

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3083 - 3083

Published: March 27, 2025

The biomechanical properties of the extracellular matrix (ECM) including its stiffness, viscoelasticity, collagen architecture, and temperature constitute critical cues governing breast cancer progression. Matrix metalloproteinase 13 (MMP13) is an important marker plays roles in remodelling cell metastasis. Emerging evidence highlights MMP13 as a dynamic modulator ECM's physical characteristics through dual mechanoregulatory mechanisms. While MMP13-mediated degradation facilitates microenvironmental softening, thus promoting tumour invasion, paradoxically, crosstalk with cancer-associated fibroblasts (CAFs) tumour-associated macrophages (TAMs) drives pathological stromal stiffening via aberrant deposition crosslinking. This duality amplified feedforward loops epithelial-mesenchymal transition (EMT) stem (CSC) populations, mediated by signalling axes such TGF-β/Runx2. Intriguingly, exhibits context-dependent mechanomodulatory effects, demonstrating anti-fibrotic activity inhibiting metastasis cancer. At same time, angiogenesis increased metabolism are mechanisms which promotes increase Targeting spatiotemporal regulation MMP13's mechanobiological functions may offer novel therapeutic strategies for disrupting tumour-stroma vicious cycle.

Language: Английский

Citations

0
Single-cell SERS imaging of dual cell membrane receptors expression influenced by extracellular matrix stiffness DOI
Xiaopeng Liu, Wenshu Zhang,

Jiahui Gu

et al.

Journal of Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 668, P. 335 - 342

Published: April 24, 2024

Language: Английский

Citations

3
Droplet-based microfluidics for engineering shape-controlled hydrogels with stiffness gradient DOI Creative Commons
Bram G. Soliman, Ian L. Chin,

Yiwei Li

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(4), P. 045026 - 045026

Published: Aug. 9, 2024

Abstract Current biofabrication strategies are limited in their ability to replicate native shape-to-function relationships, that dependent on adequate biomimicry of macroscale shape as well size and microscale spatial heterogeneity, within cell-laden hydrogels. In this study, a novel diffusion-based microfluidics platform is presented meets these needs two-step process. the first step, hydrogel-precursor solution dispersed into continuous oil phase tubing. By adjusting flow rates, physical architecture phases can be adjusted generate spherical plug-like structures, meter-long (up 1.75 m). The second step involves controlled introduction small molecule-containing aqueous through T-shaped tube connector enable molecule diffusion across interface hydrogel-precursor. Application system demonstrated by diffusing co-initiator sodium persulfate (SPS) solutions, where SPS subsequent photo-polymerization allows for formation unique radial stiffness patterns shape- size-controlled hydrogels, allowing hollow hydrogels with controllable internal architectures. Mesenchymal stromal cells successfully encapsulated containing gradient found respond heterogeneity yes-associated protein mechano-regulator. Finally, breast cancer phenotypically switch response gradients, causing shift aggregate, which may have implications metastasis. thus finds application mimicking relationship context tissue engineering provides further study roles micro- architectural features exist tissues.

Language: Английский

Citations

3
Automated Tumor and FUS Lesion Quantification on Multi-frequency Harmonic Motion and B-mode Imaging Using a Multi-modality Neural Network DOI

Shiqi Hu,

Yangpei Liu, Xiaoyue Judy Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Abstract Harmonic Motion Imaging (HMI) is an ultrasound elasticity imaging method that measures the mechanical properties of tissue using amplitude-modulated acoustic radiation force (AM-ARF). By estimating tissue’s on-axis oscillatory motion, HMI-derived displacement images represent localized relative stiffness and can predict tumor response to neoadjuvant chemotherapy (NACT) monitor focused (FUS) ablation therapy. Multi-frequency HMI (MF-HMI) excites at various AM frequencies simultaneously, which allows for image optimization without prior knowledge inclusion size stiffness. However, challenges remain in estimation as inconsistent boundary effects result different perceived sizes across frequencies. Herein, we developed automated FUS lesion quantification a transformer-based multi-modality neural network, HMINet. It was trained on 380 pairs MF-HMI B-mode phantoms vivo orthotopic breast cancer mice (4T1). Test datasets included (n = 32), 4T1 24), patients 16), FUS-induced lesion, with average segmentation accuracy (Dice Similarity Score) 0.95, 0.86, 0.82, 0.87, respectively. To increase generalizability HMINet, applied transfer learning strategy, i.e., fine-tuning model patient data. For NACT patients, ratios (DR) between surrounding were calculated based HMINet-segmented boundaries changes.

Language: Английский

Citations

1
Mechanisms of breast cancer metastasis: the role of extracellular matrix DOI
Rui Chen,

Ranqi Zhang,

Famin Ke

et al.

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 9, 2024

Language: Английский

Citations

1
Unjamming Transition as a Paradigm for Biomechanical Control of Cancer Metastasis DOI Creative Commons
Grace Cai, Nicole C. Rodgers, Allen P. Liu

et al.

Cytoskeleton, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

ABSTRACT Tumor metastasis is a complex phenomenon that poses significant challenges to current cancer therapeutics. While the biochemical signaling involved in promoting motile phenotypes well understood, role of biomechanical interactions has recently begun be incorporated into models tumor cell migration. Specifically, we propose unjamming transition, adapted from physical paradigms describing behavior granular materials, better discern transition toward an invasive phenotype. In this review, introduce jamming broadly and narrow our discussion different modes 3D migration arise. Then discuss mechanical between cells their neighbors, along with surrounding extracellular matrix. We center on induce state or these contexts. By considering interplay migration, can advance understanding control metastasis.

Language: Английский

Citations

0