Extracellular Vesicles from Plasma of Patients with Glioblastoma Promote Invasion of Glioblastoma Cells Even After Tumor Resection DOI Creative Commons
Ekaterina N. Lyukmanova, Artem V. Kirichenko, Igor Medyanik

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2834 - 2834

Published: Dec. 13, 2024

Background: Glioblastoma (GB) is a highly aggressive tumor, whose progression mediated by secretion of extracellular vesicles (EVs), which can pass the brain–blood barrier and be found in plasma. Here, we performed comparative analysis effects EVs from plasma healthy donors (hEVs) GB patients before (bEVs) after (aEVs) tumor surgical resection on invasion normal astrocytes cells. Methods: We transwell assay, analyzed MAP kinases activation Western blotting, studied SNAI1/SNAI2 cellular localization confocal microscopy, measured cadherins expression flow cytometry, cytokines, regulate migration inflammation, immunoassay. Results: hEVs did not affect cells, there was down-regulated astrocytes, while increased E- N-cadherin inflammation adhesion regulators both bEVs enhanced cells but via AKT, JNK1/2/3, p38 activation, stimulated clasterization SNAI1 cell nucleus, promoted an E/N cadherin switch, caused aEVs exhibited most pro-oncogenic (stimulation invasion, nuclear localization, JNK1/2/3 cells). However, were less pronounced than those bEVs. Conclusions: In our study, revealed common different plasma-derived hEVs, aEVs, stimulate some Being tumorigenic then bEVs, are still able to promote probably remaining resection.

Language: Английский

Electrochemical biosensors for early diagnosis of glioblastoma DOI

Hamid Vatankhahan,

Farnaz Esteki,

Mohammad Amin Jabalameli

et al.

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 557, P. 117878 - 117878

Published: March 15, 2024

Language: Английский

Citations

10
Hsp70 and Calcitonin Receptor Protein in Extracellular Vesicles from Glioblastoma Multiforme: Biomarkers with Putative Roles in Carcinogenesis and Potential for Differentiating Tumor Types DOI Open Access
Giusi Alberti, Christian M. Sánchez‐López, Antonio Marcilla

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3415 - 3415

Published: March 18, 2024

Glioblastoma multiforme (GBM) is a malignancy of bad prognosis, and advances in early detection treatment are needed. GBM heterogenous, with varieties differing within tumor patient between patients. Means needed to distinguish these GMB forms, so that specific strategies can be deployed for management. We study the participation chaperone system (CS) carcinogenesis. The CS dynamic, its members moving around body extracellular vesicles (EVs) interacting components other physiological systems health disease, including GBM. Here, we describe finding high amounts Hsp70 (HSPA1A) calcitonin receptor protein (CTR) EVs patients present standardized protocol collecting, purifying, characterizing carrying CTR plasma-derived from were obtained just before ablative surgery (T0) 7 days afterwards (T1); was highly elevated at T0 less T1, greatly increased reduced below normal values T1. Our results encourage further research assess as biomarkers differentiating forms determine their roles

Language: Английский

Citations

6
Role of Extracellular Vesicles in the Progression of Brain Tumors DOI Creative Commons
Gabriella Schiera, Carlo Maria Di Liegro,

Francesco Vento

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(8), P. 586 - 586

Published: Aug. 2, 2024

Brain tumors, and, in particular, glioblastoma (GBM), are among the most aggressive forms of cancer. In spite advancement available therapies, both diagnosis and treatments still unable to ensure pathology-free survival GBM patients for more than 12–15 months. At basis poor ability cope with brain we can consider: (i) intra-tumor heterogeneity; (ii) heterogeneity tumor properties when compare different patients; (iii) blood–brain barrier (BBB), which makes difficult isolation tumor-specific biomarkers delivering therapeutic drugs brain. Recently, it is becoming increasingly clear that cancer cells release large amounts extracellular vesicles (EVs) transport metabolites, proteins, classes RNAs, DNA, lipids. These structures involved pathological process characterize any particular form Moreover, EVs able cross BBB directions. Starting from these observations, researchers now evaluating possibility use purified organic fluids (first all, blood saliva), order obtain, through non-invasive methods (liquid biopsy), biomarkers, perhaps, also obtaining nanocarriers targeted drugs.

Language: Английский

Citations

3
Nucleic acid nanobiosystems for cancer theranostics: an overview of emerging trends and challenges DOI

Laura P. Rebolledo,

Luciana Nogueira de Sousa Andrade, Marcio C. Bajgelman

et al.

Nanomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: May 6, 2025

Different cancers remain major global health challenges due to their diverse biological behaviors and significant treatment hurdles. The aging of populations lifestyle factors increase cancer occurrence place increasing pressure on healthcare systems. Despite continuous advancements, many fatal late-stage diagnosis, tumor heterogeneity, drug resistance, thus necessitating urgent development innovative solutions. Therapeutic nucleic acids, a new class drugs, offer promising approach overcoming these challenges. recent Nucleic Acids Nanobiosystems in Cancer Theranostics (NANCT) conference brought together internationally recognized experts from 15 countries discuss cutting-edge research, spanning oncolytic viruses anticancer RNA nanoparticles other emerging nanotechnologies. This review captures key insights developments, emphasizing the need for interdisciplinary translation scientific advancements into clinical practice shaping future personalized treatments improved therapeutic outcomes.

Language: Английский

Citations

0
Extracellular Vesicles: Tiny Messengers for Mighty RNA Delivery DOI Creative Commons
Alakesh Das

Biologics, Journal Year: 2024, Volume and Issue: 4(1), P. 88 - 104

Published: March 6, 2024

Extracellular vesicles (EVs) encompass a diverse array of cell-derived vesicles, originating either from the endosomal compartment (exosomes) or generated through shedding cell membrane. These lipid bilayer nanovesicles carry cargo consisting nucleic acids, various macromolecules, and growth factors, capable being assimilated by nearby distant cells biofluids, thereby triggering wide range cellular responses. Given their distinctive biological characteristics crucial roles in intercellular communication, EVs have garnered significant attention, especially concerning potential clinical applications. Inheriting parent cells, present promising resources for disease biomarkers. Research elucidating specific impacts on target has sparked enthusiasm therapeutic potential. Compelling evidence indicates that RNA housed within can modulate gene expression influence functions recipient cells. However, despite progress, numerous aspects EV biology remain obscure, encompassing selective cargo-loading mechanisms yield distinct compositions source variability size content, undisclosed pathways governing uptake fate A thorough understanding core mechanisms—such as generation, trafficking, payload delivery—is essential effective utilization. This review explores current loading transportation EVs, light advancements made toward

Language: Английский

Citations

2
Implications of glioblastoma-derived exosomes in modifying the immune system: state-of-the-art and challenges DOI

Yashmin Afshar,

Negin Sharifi,

Amirhossein Kamroo

et al.

Reviews in the Neurosciences, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Glioblastoma is a brain cancer with poor prognosis. Failure of classical chemotherapy and surgical treatments indicates that new therapeutic approaches are needed. Among cell-free options, exosomes versatile extracellular vesicles (EVs) carry important cargo across barriers such as the blood-brain barrier (BBB) to their target cells. This makes an interesting option for treatment glioblastoma. Moreover, can comprise many cargos, including lipids, proteins, nucleic acids, sampled from special intercellular compartments origin cell. Cells exposed various immunomodulatory stimuli generate enriched in specific molecules. Notably, secretion could modify immune response innate adaptive systems. For instance, glioblastoma-associated (GBex) uptake by macrophages influence macrophage dynamics (e.g., shifting CD markers expression). Expression critical immunoregulatory proteins cytotoxic T-lymphocyte antigen-1 (CTLA1) programmed death-1 (PD-1) on GBex direct crosstalk these nano-size system. The present study reviews role system cells, B T natural killer (NK) dendritic cells (DCs), well novel technologies field.

Language: Английский

Citations

0
Extracellular Vesicles from Plasma of Patients with Glioblastoma Promote Invasion of Glioblastoma Cells Even After Tumor Resection DOI Creative Commons
Ekaterina N. Lyukmanova, Artem V. Kirichenko, Igor Medyanik

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2834 - 2834

Published: Dec. 13, 2024

Background: Glioblastoma (GB) is a highly aggressive tumor, whose progression mediated by secretion of extracellular vesicles (EVs), which can pass the brain–blood barrier and be found in plasma. Here, we performed comparative analysis effects EVs from plasma healthy donors (hEVs) GB patients before (bEVs) after (aEVs) tumor surgical resection on invasion normal astrocytes cells. Methods: We transwell assay, analyzed MAP kinases activation Western blotting, studied SNAI1/SNAI2 cellular localization confocal microscopy, measured cadherins expression flow cytometry, cytokines, regulate migration inflammation, immunoassay. Results: hEVs did not affect cells, there was down-regulated astrocytes, while increased E- N-cadherin inflammation adhesion regulators both bEVs enhanced cells but via AKT, JNK1/2/3, p38 activation, stimulated clasterization SNAI1 cell nucleus, promoted an E/N cadherin switch, caused aEVs exhibited most pro-oncogenic (stimulation invasion, nuclear localization, JNK1/2/3 cells). However, were less pronounced than those bEVs. Conclusions: In our study, revealed common different plasma-derived hEVs, aEVs, stimulate some Being tumorigenic then bEVs, are still able to promote probably remaining resection.

Language: Английский

Citations

0