Anticancer and Antioxidant Effects of Bioactive Peptides from Black Soldier Fly Larvae (Hermetia illucens)

Nutrients, Journal Year: 2025, Volume and Issue: 17(4), P. 645 - 645

Published: Feb. 11, 2025

Background: Protein hydrolysates from insects are recognized for their biological activities. Black soldier fly larvae (BSFL) have drawn attention due to antioxidant protein hydrolysates. However, research on bioactive peptides derived these hydrolysates, particularly cancer chemopreventive potential, remains limited. This study aims evaluate the antioxidant, anti-inflammatory, antimutagenic, and anticancer activities of BSFL-derived explore molecular mechanisms. Methods: Alkali-soluble BSFL (ASBP) was extracted hydrolyzed using Alcalase bromelain under optimized conditions. Antioxidant activity assessed via FRAP, ABTS, DPPH assays. The hydrolysate with highest fractionated into weight (MW) groups (>30, 10, <3 kDa). bioactivity evaluated through anti-inflammatory (nitric oxide production in RAW 264.7 cells), antimutagenic (Ames test), (CCK-8 assay HCT 116, COLO205, Cw-2, Caco-2 cells) Mechanistic insights were obtained microarray Western blot analyses. Peptides identified by LC-MS/MS. Results: ASBP-Alcalase (ASBP-AH) showed optimal at 3% (w/w) 4 h. ASBP-AH 30 (MW > kDa) fraction exhibited capacity. In contrast, ASBP-AH3 < 3 significant effects, reduced nitric production, decreased COLO205 cell viability. Treatment its LC50 dose modulated SKP2/p21/cyclin D1 pathways. Mostly composed hydrophobic charged amino acids. Conclusions: exhibit potential as multifunctional agents chemoprevention. vivo studies required clinical applications.

Language: Английский

Targeting of S-phase kinase associated protein 2 stabilized tumor suppressors leading to apoptotic cell death in squamous skin cancer cells

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(7), P. 167286 - 167286

Published: June 12, 2024

Language: Английский

A patent review of SCF E3 ligases inhibitors for cancer：Structural design, pharmacological activities and structure–activity relationship

European Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 278, P. 116821 - 116821

Published: Aug. 31, 2024

Language: Английский