International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 663 - 663
Published: Jan. 14, 2025
Organoid technology, as an innovative approach in biomedicine, exhibits promising prospects disease modeling, pharmaceutical screening, regenerative medicine, and oncology research. However, the use of tumor-derived Matrigel primary method for culturing organoids has significantly impeded clinical translation organoid technology due to concerns about potential risks, batch-to-batch instability, high costs. To address these challenges, this study innovatively introduced a photo-crosslinkable hydrogel made from porcine small intestinal submucosa decellularized matrix (SIS), fish collagen (FC), methacrylate gelatin (GelMA). The cost-effective demonstrated excellent biocompatibility, tunable mechanical properties, rapid gelation low immunogenicity. Importantly, proliferation differentiation capacities cultured were comparable those Matrigel, with no significant disparity observed. Furthermore, after one week transplantation nude mice, hydrogel-organoid complex exhibited sustained structural functional stability while preserving characteristics organoids. Our also effective FC/SIS/GelMA accelerating repair process defects, reducing area scar formation, promoting regeneration both villi smooth muscle tissue. In summary, presents novel protocol organoids, offering implications future applications serving experimental foundation development tissue-engineered intestines based on
Language: Английский
Citations
1
Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 151730 - 151730
Published: March 1, 2025
Language: Английский
Citations
0
Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 457 - 457
Published: March 19, 2025
Colorectal epithelium was the first long-term 3D organoid culture established in vitro. Identification of key components essential for survival stem cell niche allowed an indefinite propagation these cultures and modulation their differentiation into various lineages mature intestinal epithelial cells. While methods were eventually adapted to establish organoids from different organs, colorectal remain a pioneering model development new applications health disease. Several basic applicative aspects culture, modeling, monitoring testing are analyzed this review. We also tackle ethical problems biobanking distribution precious research tools, frequently confined laboratory origin or condemned destruction at end project.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 16, 2024
Abstract Over the past decade, intestinal organoids have shown great promise as a platform to study development of intestine, elucidate pathogenesis diseases such inflammatory bowel disease (IBD), and model physiological features tissue for high-throughput drug screening. However, derived from adult epithelial stem cells lack cellular diversity, especially those resides in lamina propria. Intestinal embryonic (ES) or induced-pluripotent (iPSCs) has greater but are still limited size peristalsis. In this study, we generated centimeter-scale, full-thickness, physiologically relevant suspension culture without usage Matrigel. Using series optimized media, multiple lineages were differentiated iPSCs spontaneously assembled achieve architecture. These bubble-like thickness 500 microns, exhibit mature vasculature network, smooth muscle-like conduct regular addition, adipocyte-like granulocyte-like also observed these organoids, which important immune homeostasis. Lastly, show crypt structures, response lipopolysaccharides (LPS), increases luminal influx upon forskolin treatment, suggesting intact integrity. Thus, provides highly reproducible approach produce large that suitable different biomedical applications.
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0