IL-23 Promotes γδT Cell Activity in Dry Eye Disease Progression

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(2), P. 10 - 10

Published: Feb. 4, 2025

Purpose: Conjunctival-resident γδT cells, the predominant ocular source of interleukin-17A (IL-17A), play crucial roles in dry eye disease (DED) pathogenesis. The upstream regulators these cells are unknown. This study evaluated role conjunctival IL-23 expression mediating cell generation and elucidated its contribution to inflammatory responses. Methods: Single-cell RNA sequencing (scRNA-seq) was used identify quantify mRNA molecules mice. level increased wild-type (WT) decreased γδT-deficient (TCRδ–/–) mice after induced via an intelligently controlled environmental system (ICES). Flow cytometry transcriptome were investigate impact changes on human cells. Results: receptor (IL-23R) greater than other types, such as CD4+ T CD8+ epithelial An increase led density, which proportional severity. However, TCRδ–/– mice, upregulation failed IL-17A severity eye. Furthermore, increases number evident surface patients who developed visual display terminal syndrome. Conclusions: contributes induction DED response through interactions with cognate promotion their proliferation. findings this suggest that suppression blockade IL-23R activation may be a viable target for improving management inflammation patients.

Language: Английский

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Comprehensive biomarker profiles in hematological malignancies: improving diagnosis, prognosis, and treatment

Biomarkers in Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 16

Published: Feb. 27, 2025

Hematological malignancies present substantial challenges in clinical practice due to their heterogeneity and complex biological profiles. In these diseases, biomarkers – measurable indicators of states are indispensable for diagnosis, prognosis, therapeutic decision-making. Emerging significantly improving outcomes hematological cancers by enhancing early detection, refining prognostic assessments, enabling personalized treatment approaches, optimizing overall patient management. This progress translates into better more effective strategies treat manage malignancies. The field biomarker discovery has developed from basic morphological cytogenetic markers advanced molecular techniques, including polymerase chain reaction (PCR) next-generation sequencing (NGS), which have enhanced diagnostic accuracy led the development targeted therapies. Additionally, recent advent technologies like mass spectrometry single-cell RNA enables comprehensive profiling reveals novel that were previously undetectable. Our aim this manuscript is provide a overview immunohematological biomarkers, applications, future directions field.

Language: Английский

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Prognostic value of natural killer T cell related genes in acute myeloid leukemia

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 13, 2025

Acute myeloid leukemia (AML) is a hematological malignancy characterized by complex immune microenvironment. This study aims to identify immune-related prognostic biomarkers in AML. Multiple public sequencing datasets were utilized analyze differentially expressed genes (DEGs) Single-sample gene set enrichment analysis (ssGSEA) and weighted co-expression network (WGCNA) also performed. Immune cell infiltration was assessed at the single-cell level. NKT marker intersected with most AML-relevant module key genes. Prognostic screened using Cox Lasso regression model, their value evaluated random forest Kaplan-Meier survival analyses. Gene expression validated RT-qPCR Western blot, levels analyzed flow cytometry. A total of 1,919 common DEGs obtained between AML controls. WGCNA revealed that brown strongly associated Single-cell showed significantly reduced patients, consistent ssGSEA results. Forty intersecting identified 10 (FGFBP2, GZMB, GZMH, IKZF3, IL2RB, KLRB1, KLRC2, RHOF, RUNX3, STAT4). risk score model based on these stratified patients into high-risk low-risk groups, significant differences prognosis two groups. blot analyses downregulated patients. Flow cytometry results lower CD8 + T cells compared highlighted critical role pathogenesis. The provides new insights potential for understanding biology, prognosis, therapeutic targets.

Language: Английский

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Advances in DNA/RNA Sequencing and Their Applications in Acute Myeloid Leukemia (AML)

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 71 - 71

Published: Dec. 25, 2024

Acute myeloid leukemia (AML) is an aggressive malignancy that poses significant challenges due to high rates of relapse and resistance treatment, particularly in older populations. While therapeutic advances have been made, survival outcomes remain suboptimal. The evolution DNA RNA sequencing technologies, including whole-genome (WGS), whole-exome (WES), (RNA-Seq), has significantly enhanced our understanding AML at the molecular level. These technologies led discovery driver mutations transcriptomic alterations critical for improving diagnosis, prognosis, personalized therapy development. Furthermore, single-cell (scRNA-Seq) uncovered rare subpopulations stem cells (LSCs) contributing disease progression relapse. However, widespread clinical integration these tools remains limited by costs, data complexity, ethical challenges. This review explores recent advancements DNA/RNA highlights both potential limitations techniques practice.

Language: Английский

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