International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113848 - 113848
Published: Dec. 17, 2024
Language: Английский
Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)
Published: Dec. 18, 2024
Abstract Background Organoids, as near-physiological 3D culture systems, offer new opportunities to study the pathogenesis of various organs in mimicking cellular complexity and functionality human organs. Method Here we used a quite simple very practicable method successfully generate induced pluripotent stem cell (iPSC)-derived lung organoids (LuOrg) matrix-free manner an alternative widely preclinical mouse models order investigate normal damage detail close possible patient. We performed detailed morphological molecular analyses, including bulk single RNA sequencing, generated evaluated quality robustness our model potential vitro platform for diseases, namely radiation-induced injury. Results A differentiation iPSCs can be obtain that morphologically reflect target tissue well, especially with regard composition. The different fates were investigated following genotoxic stress by radiation revealed further insights radiation-sensitivity cells. Finally, provide gene sets found organoid subsets after irradiation, which could additional RT response particularly senescence future studies. Conclusion By establishing these free-floating LuOrgs investigation cancer therapeutic approaches patient-oriented experimental radiooncology, not only reduction number animals, but also adequately meaningfully replacement corresponding animal experiments achieved.
Language: Английский
Citations
2
Stem Cells International, Journal Year: 2024, Volume and Issue: 2024(1)
Published: Jan. 1, 2024
Background: Endometrial injury contributes to the reduction of endometrial receptivity and is widely recognized as a critical factor in implantation failure. Increasing evidence suggests that therapeutic effects mesenchymal stem cells (MSCs) mainly depend on their capacity secrete paracrine factors specifically MSC‐derived exosomes (MSC‐Exos), which are regulated by exosomal miRNA. In this study, we investigated human umbilical cord MSC‐Exos (hUCMSC‐Exos) injured endometrium potential mechanisms. Methods: To observe distribution vivo, DIR‐labeled hUCMSC‐Exos were injected into tail vein endometrium‐injured mice. The recovery mice damaged after exosome treatment was detected hematoxylin–eosin (HE), TUNEL staining, immunohistochemistry. Then, western blot measured expression Bcl‐2, Bax, cleaved caspase‐3. mRNA vascular endothelial growth (VEGF) insulin‐like factor‐1 (IGF‐1) evaluated quantitative real‐time PCR (qRT‐PCR). Furthermore, miR‐21‐5p checked mifepristone‐injured stromal (EndoSC) addition. cell viability apoptosis analyzed phosphatase tensin homolog deleted chromosome 10 (PTEN)/serine/threonine kinase (AKT) pathway confirmed with transfected miR‐21‐5p. Results: migrated endometrium, thickness, number glands well embryo rate significantly increased ( p < 0.05). could inhibit our mouse model. Besides, Ki67 cluster differentiation 31 (CD31) VEGF IGF‐1 upregulated group repair effect further enhanced transfection mimics counteracted inhibitors. related decreased PTEN level activated PI3K/AKT signaling pathway. Conclusion: ameliorated uterus, rates, suppressed apoptosis, improved proliferation Exosomal positively enhances activate PTEN/AKT pathways.
Language: Английский
Citations
0
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113848 - 113848
Published: Dec. 17, 2024
Language: Английский
Citations
0