Comprehensive Single‐Cell RNA Sequencing Analysis of Cervical Cancer: Insights Into Tumor Microenvironment and Gene Expression Dynamics

International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Cervical cancer is a complex disease with considerable cellular heterogeneity, which hampers our understanding of its progression and the development effective treatments. Single‐cell RNA sequencing (scRNA‐seq)—a technology that enables gene expression analysis at level—has emerged as an important tool to explore this heterogeneity on cell‐to‐cell basis. We perform data quality differential in cervical via scRNA‐seq, giving insights into tumor microenvironment likely therapeutic targets. Methods: scRNA‐seq for sample advanced bioinformatics were utilized. Scatter plots generated assess control metrics based mitochondrial total count. Cell clustering identified significant genes each cell cluster. Gene coexpression networks modules performed network analysis. utilized pseudotime model experience state transitions infer trajectory functional enrichment understand biological processes involved. Results: revealed distinct cluster pattern high profile. Ultimately, suggested genes: TP53, GNG4, CCL5 had degrees potential roles progression. Some these have unique functions by analysis, while dynamic changes across reveal differences during transition. next immune response metabolic play pivotal role cancer. Conclusion: Our large scale provide dynamics within microenvironment.

Language: Английский

Enhanced Expression of N-Cadherin, but Not of E-Cadherin, in Cutaneous Squamous Cell Carcinoma in Comparison to Basal Cell Carcinoma

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4247 - 4247

Published: Dec. 20, 2024

Background: Adhesion molecules including E-cadherin and N-cadherin have been proven to contribute the carcinogenesis process. It has demonstrated that an increased expression or appearance of N-cadherin, as well a reduction in E-cadherin, are documented many cancers, often leading loss intercellular adhesion acquisition more invasive even metastatic mesenchymal phenotype. The aim this study was assess markers proliferation Ki67 basal cell carcinoma (BCC) squamous (SCC). Methods: A total 123 tumor paraffin specimens, 73 BCC 50 SCC cases, were obtained from multiple anatomical locations. E-Cadherin N-Cadherin, percentage stained cells, assessed using four-grade scale, with Ki-67 on five-grade scale. Results: significantly higher observed compared BCC, 14% cases having than 50% 10% 26–50% expression, comparison 2.7% 8.2% respectively (p < 0.001). No significant differences regard between BCC. Conclusions: Our results suggest might phenotype, SCC, when high both tumors explaining their overall low rate metastases; however, further research role these is needed.

Language: Английский