A genome and tissue-specific transcriptomes of the large-polyp coral,Fimbriaphyllia(Euphyllia)ancora: Recipe for a coral polyp DOI Creative Commons
Shinya Shikina, Yuki Yoshioka,

Yi‐Ling Chiu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Coral “polyps” are composed of several tissues; however, their characteristics largely unexplored. Here we report biological the four tissues that comprise polyps: tentacle ( Te ), mesenterial filament Me body wall Bo) , and mouth with pharynx MP using comparative genomic, morpho-histological, transcriptomic analyses large-polyp coral, Fimbriaphyllia ancora . A draft F. genome assembly 434 Mbp was created. Morpho-histological characterization showed they have distinct differences in structure, primary cellular composition, transcriptional profiles. Tissue-specific, highly expressed genes (HEGs) related to defense, predation, coral-algal symbiosis. expresses multiple digestive enzymes, whereas Bo innate immunity biomineralization-related molecules. Many receptors for neuropeptides neurotransmitters The established dataset new insights into tissue functions will facilitate a deeper understanding coral biology.

Language: Английский

Melanopsin-mediated optical entrainment regulates circadian rhythms in vertebrates DOI Creative Commons
Deng Pan, Zixu Wang, Yaoxing Chen

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: Oct. 18, 2023

Melanopsin (OPN4) is a light-sensitive protein that plays vital role in the regulation of circadian rhythms and other nonvisual functions. Current research on OPN4 has focused mammals; more evidence needed from non-mammalian vertebrates to fully assess significance non-visual photosensitization for rhythm regulation. There are species differences regulatory mechanisms vertebrate rhythms, which may be due cutting variants, tissue localization, photosensitive activation pathway OPN4. We here summarize distribution mammals, birds, teleost fish, classical excitation mode function mammals discussed. In addition, OPN4-expressing cells regulating different highlighted, potential rhythmic effects various neuropeptides or neurotransmitters expressed mammalian ganglion summarized among them.

Language: Английский

Citations

6
Beyond Lux: Methods for Species and Photoreceptor-Specific Quantification of Ambient Light for Mammals DOI Creative Commons
Richard J. McDowell, Altuğ Didikoğlu, Tom Woelders

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 27, 2023

Abstract Background Light is a key environmental regulator of physiology and behaviour. Mistimed or insufficient light disrupts circadian rhythms associated with impaired health well-being across mammals. Appropriate lighting therefore crucial for indoor housed The most commonly used measurement lux. However, this employs spectral weighting function based on human perceived brightness not suitable ‘non-visual’ effects use species. In humans, photoreceptor-specific (α-opic) metrology system has been proposed as more appropriate way measuring light. Results Here we establish technology to allow α-opic approach be readily extended any mammalian species, accounting differences in photoreceptor types, photopigment sensitivities, eye anatomy. Since sensitivity can hard derive novel animals photoreceptors, developed high-throughput, easy-to-use, method sensitivities recombinantly expressed melanopsins it the melanopsin from 12 non-human We further address need simple strategies species-specific measures by developing an accessible online toolbox calculating these units validating open hardware, low-cost, multichannel sensor ‘point click’ measurement. finally demonstrate that measurements are superior photopic lux predictors physiological responses mice ecologically relevant comparisons photosensitivity between Conclusion Our study demonstrates accurately using existing unit holds promise improvements welfare animals, scientific research reproducibility, agricultural productivity, energy usage.

Language: Английский

Citations

5
Beyond Lux: methods for species and photoreceptor-specific quantification of ambient light for mammals DOI Creative Commons
Richard J. McDowell, Altuğ Didikoğlu, Tom Woelders

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 14, 2024

Abstract Background Light is a key environmental regulator of physiology and behaviour. Mistimed or insufficient light disrupts circadian rhythms associated with impaired health well-being across mammals. Appropriate lighting therefore crucial for indoor housed commonly measured in lux. However, this employs spectral weighting function human luminance not suitable ‘non-visual’ effects use species. In humans, photoreceptor-specific (α-opic) metrology system has been proposed as more appropriate way measuring light. Results Here we establish technology to allow α-opic measurement approach be readily extended mammalian species, accounting differences photoreceptor types, photopigment sensitivities, eye anatomy. We develop high-throughput method derive sensitivities recombinantly expressed opsins it the sensitivity melanopsin from 13 non-human further address need simple strategies species-specific measures by developing an accessible online toolbox calculating these units validating open hardware multichannel sensor ‘point click’ measurement. finally demonstrate that measurements are superior photopic lux predictors physiological responses mice ecologically relevant comparisons photosensitivity between Conclusions Our study presents methods existing unit holds promise improvements welfare animals, scientific research reproducibility, agricultural productivity, energy usage.

Language: Английский

Citations

1
Genome and tissue-specific transcriptomes of the large-polyp coral, Fimbriaphyllia (Euphyllia) ancora: a recipe for a coral polyp DOI Creative Commons
Shinya Shikina, Yuki Yoshioka,

Yi‐Ling Chiu

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: July 24, 2024

Abstract Coral polyps are composed of four tissues; however, their characteristics largely unexplored. Here we report biological tentacles ( Te ), mesenterial filaments Me body wall Bo) , and mouth with pharynx MP using comparative genomic, morpho-histological, transcriptomic analyses the large-polyp coral, Fimbriaphyllia ancora . A draft F. genome assembly 434 Mbp was created. Morpho-histological characterization tissues showed that they have distinct differences in structure, primary cellular composition, transcriptional profiles. Tissue-specific, highly expressed genes (HEGs) related to defense, predation, coral-algal symbiosis. expresses multiple digestive enzymes, whereas Bo innate immunity biomineralization-related molecules. Many receptors for neuropeptides neurotransmitters This dataset new insights into tissue functions will facilitate a deeper understanding symbiotic biology, immunology, biomineralization, neurobiology corals.

Language: Английский

Citations

0
A genome and tissue-specific transcriptomes of the large-polyp coral,Fimbriaphyllia(Euphyllia)ancora: Recipe for a coral polyp DOI Creative Commons
Shinya Shikina, Yuki Yoshioka,

Yi‐Ling Chiu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Coral “polyps” are composed of several tissues; however, their characteristics largely unexplored. Here we report biological the four tissues that comprise polyps: tentacle ( Te ), mesenterial filament Me body wall Bo) , and mouth with pharynx MP using comparative genomic, morpho-histological, transcriptomic analyses large-polyp coral, Fimbriaphyllia ancora . A draft F. genome assembly 434 Mbp was created. Morpho-histological characterization showed they have distinct differences in structure, primary cellular composition, transcriptional profiles. Tissue-specific, highly expressed genes (HEGs) related to defense, predation, coral-algal symbiosis. expresses multiple digestive enzymes, whereas Bo innate immunity biomineralization-related molecules. Many receptors for neuropeptides neurotransmitters The established dataset new insights into tissue functions will facilitate a deeper understanding coral biology.

Language: Английский

Citations

1