Arabian Journal of Chemistry,
Journal Year:
2024,
Volume and Issue:
17(3), P. 105625 - 105625
Published: Jan. 17, 2024
Ulcerative
colitis
(UC)
is
an
autoimmune
disease
with
a
steady
increase
in
global
prevalence
and
long-term
susceptibility
to
colorectal
cancer
(CRC).
CRC
often
treated
XELOX
regimen
(oxaliplatin
capecitabine),
which
limited
by
the
high
toxicity,
many
adverse
reactions
intolerance
of
patients,
thus
leading
termination
chemotherapy.
Traditional
Chinese
medicine
effective
improving
patients'
clinical
symptoms.
Gegen
Qinlian
decoction
(GQD)
frequently
utilized
management
UC
has
potential
treat
CRC.
Whereas,
there
are
few
studies
on
GQD
treatment
UC-associated
CRC,
therapeutic
mechanism
combined
not
been
fully
reported.
Herein,
HPLC-Q-TOF-MS/MS
technique
was
used
analyze
main
chemical
components
GQD.
Network
pharmacology
performed
unveil
critical
genes
against
The
detection
TNF-α,
IL-1β,
IL-6,
VEGF,
SOD,
MDA
immune
factors
revealed
that
played
key
role
function,
reducing
inflammation
resisting
oxidative
stress.
16S
rDNA
sequencing
technology
results
showed
could
maintain
gastrointestinal
homeostasis
increasing
beneficial
bacteria
decreasing
harmful
bacteria.
Then,
metabolomics
based
found
combination
significantly
restore
disturbance
metabolites.
Particularly,
compound-reaction-gene-enzyme
network
first
constructed
realize
metabolomics.
may
assist
play
synergistic
anti-tumor
regulating
enzymes
ALOX15,
CYP1B1
PTGS2
unsaturated
fatty
acid
metabolism.
Finally,
verified
western
blot,
pharmacodynamic
were
molecular
docking.
Overall,
current
study
offered
fresh
perspectives
both
prevention
as
well
concepts
for
application
lessen
side
effects
chemotherapy
enhance
quality
life.
Pharmacological Research - Modern Chinese Medicine,
Journal Year:
2024,
Volume and Issue:
10, P. 100366 - 100366
Published: Jan. 19, 2024
Huachansu
injection
(HCS)
is
an
aqueous
extract
preparation
of
Traditional
Chinese
Medicine
(TCM)
toad
skin
(Bufo
melanosticus
or
B.
bufo
gargarizans).
As
a
national
second-class
and
self-developed
TCM
preparation,
it
widely
used
to
treat
human
colorectal
cancer
(CRC),
yet
its
substantial
toxic
side
effects,
such
as
vascular
irritation
reaction,
drug
fever,
allergic
reactions,
pose
considerable
challenge
during
clinical
use.
In-depth
research
on
the
material
basis
HCS'
traditional
efficacy
essential
for
developing
more
effective
less
medicine.
Identifying
main
components
responsible
HCS
crucial
in
determining
explaining
any
adverse
reactions.
The
purpose
this
study
clarify
active
growth
inhibitory
agent
against
CRC
HCT116
cells
further
understand
anti-CRC
properties.
Sulforhodamine
B
(SRB)
assay
was
screen
compound
with
strong
activity
from
44
compounds
isolated
extracts
(B.
melanosticus).
screened
standard
MeOH
were
determined
by
HPLC
coupled
triple
quadrupole
mass
spectrometry.
partial
subjected
system
using
solvents
gradient
increasing
polarity,
yielding
12
fractions
total
(HCS1
HCS12).
Besides,
knocked
out
obtain
fraction
negative
control,
knocked-out
collected
one
semi-preparative
HPLC.
Those
submitted
HPLC-MS/MS
HPLC-DAD
determine
presence
absence
compounds.
In
addition,
cytotoxicity
those
prepared
evaluated
effect
HCS.
Six
compounds,
namely
arenobufagin,
telocinobufagin,
gamabufalin,
hellebrigenin,
19-hydroxylbufagin
argentinogenin,
their
(with
IC50
value
than
400
nM),
present
at
total-mass-percentage
1.35×10−3%
extract.
6
mainly
distributed
HCS8,
which
also
showed
strongest
cells.
And
results
multicomponent
knockout
indicated
control
lost
significantly,
while
can
be
restored
after
added
control.
six
bufadienolides
derivatives
are
HCS,
anti-CRC.
Through
research,
we
clearly
work
lays
foundation
development
medicine
Frontiers in Pharmacology,
Journal Year:
2024,
Volume and Issue:
15
Published: June 10, 2024
Over
the
past
few
years,
there
has
been
a
gradual
increase
in
incidence
of
cancer,
affecting
individuals
at
younger
ages.
With
its
refractory
nature
and
substantial
fatality
rate,
cancer
presents
notable
peril
to
human
existence
wellbeing.
Hawthorn,
medicinal
food
homology
plant
belonging
Crataegus
genus
Rosaceae
family,
holds
great
value
various
applications.
Due
long
history
use,
effects,
high
safety
profile,
hawthorn
garnered
considerable
attention
plays
crucial
role
treatment.
Through
integration
modern
network
pharmacology
technology
traditional
Chinese
medicine
(TCM),
range
anticancer
active
ingredients
have
predicted,
identified,
analyzed.
Studies
shown
that
such
as
vitexin,
isoorientin,
ursolic
acid,
maslinic
along
with
extracts,
can
effectively
modulate
cancer-related
signaling
pathways
manifest
properties
via
diverse
mechanisms.
This
review
employs
excavate
potential
hawthorn.
By
systematically
integrating
literature
across
databases
PubMed
CNKI,
explores
bioactive
underlying
mechanisms
pathways,
synergistic
effects
drug
combinations,
advancements
novel
delivery
systems,
ongoing
clinical
trials
concerning
hawthorn’s
properties.
Furthermore,
highlights
preventive
health
benefits
prevention,
offering
valuable
insights
for
treatment
development
TCM
be
used
both
edible
purposes.
