Protective effects of Lactobacillus plantarum strain against protein malnutrition-induced muscle atrophy and bone loss in juvenile mice

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0317197 - e0317197

Published: Jan. 16, 2025

Early-life malnutrition adversely affects nearly all organ systems, resulting in multiple physiological adaptations, including growth restriction and muscle bone loss. Although there is growing evidence that probiotics effectively improve systemic under malnourished conditions different animal models, our knowledge of the beneficial effects on various organs limited. Here, we show Lactobacillus plantarum strain WJL (LpWJL) can mitigate skeletal loss protein-malnourished juvenile mice. Mice prenatal day 21 were fed a (P-MAL) diet with or without LpWJL supplementation for six weeks. Compared to mice P-MAL alone, significantly increased mass size, enhanced strength endurance capacity. Furthermore, induced expression key factor IGF-1 while decreasing atrophy markers such as Atrogin-1 MuRF-1, indicating potential mechanisms by which protein malnutrition-induced wasting counteracted. Additionally, alleviated reduction cortical thickness deterioration trabecular microstructure femur. Taken together, these results indicate protect against compromised microarchitecture caused malnutrition, providing novel insights into therapeutic applications treating malnutrition-related disorders.

Language: Английский

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The Alleviative Effect of Sodium Butyrate on Dexamethasone‐Induced Skeletal Muscle Atrophy

Cell Biology International, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

ABSTRACT Skeletal muscle mass is significantly negatively regulated by glucocorticoids. Following glucocorticoid administration, the balance between protein synthesis and breakdown in skeletal disrupted, shifting towards a predominance of catabolic metabolism. Short‐chain fatty acids like sodium butyrate have been found to regulate inflammatory reactions successively activate signaling pathways. The preventive benefits against dexamethasone‐induced atrophy myotube models were examined this work, underlying mechanism was clarified. A total 32 6‐week‐old C57BL/6 inbred male mice randomly assigned one four groups treated with dexamethasone induce butyrate. We that succinate alleviated model lowering gene expression two E3 ubiquitin ligases, Atrogin‐1 MURF1, activating AKT/mTOR pathway. Pertussis toxin reversed effect, indicating G protein‐coupled receptors involved butyrate's action as mediator. Additionally, pre‐treatment lowered weight loss mouse atrophy, dramatically decreased MURF1 nuclear translocation receptor. In conclusion, study shows inhibits genes, thus preventing proteins mass, while also inhibiting loss, animal models.

Language: Английский

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Effect of Intraoperative Dexmedetomidine on Early Attention Network Function After Gynaecological Surgery: A Randomized Controlled Study

Basic & Clinical Pharmacology & Toxicology, Journal Year: 2025, Volume and Issue: 136(5)

Published: April 4, 2025

Perioperative neurocognitive disorder (PND) is a common neurological complication in patients after surgery and anaesthesia. Whether dexmedetomidine affects postoperative attention network function remains unclear. Eighty aged 40-60 years underwent elective gynaecological under total intravenous anaesthesia, before induction (group D) or placebo P) was used. The test used to assess the of three networks pre- post-operation, blood samples were collected inflammatory factors neurotransmitters. two groups obviously impaired operation. Horizontally, on 1st day, degree impairment alerting group D less than that P (p = 0.033), orienting completely protected < 0.001, vs. P; p 0.058, baseline), while executive control improved 0.045, baseline). Moreover, all levels increased day. In contrast, acetylcholine (ACh) dopamine (DA) decreased significantly 0.049 for ACh, 0.001 DA). D, serum neurotransmitters showed different patterns. Dexmedetomidine can protect against early middle-aged female patients. This trial has been registered with Chinese Clinical Trial Registry (https://www.chictr.org.cn) (ChiCTR2000031283).

Language: Английский

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Effects of Thyroid Hormones on Cellular Development in Human Ovarian Granulosa Tumor Cells (KGN)

Reproductive Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Language: Английский

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Inflammation-related proteomics demonstrate landscape of fracture blister fluid in patients with acute compartment syndrome

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 6, 2023

Background Blisters are tense vesicles or bullae that arise on swollen skin and found in a wide range of injuries. As complication fracture, fracture blisters considered soft tissue injuries, which often lead to adverse effects such as prolonged preoperative waiting time increased risk surgical site infection. However, our previous study patients with acute compartment syndrome, may be form pressure release, but the specific mechanism has not been revealed. Here, we mapped out proteomic landscape blister fluid for first compared its expression profile cupping burn blisters. Methods First, samples were collected from 15 blisters, 7 9 Then, levels 92 inflammatory proteins measured using Olink Target 96 Inflammation panel. Protein profiles across three groups Differential Expression Analysis Principal Component (PCA). Results Fracture had significantly higher 50 comparison 26 Notably, PCA showed closely resembled protein distinct Conclusion Our provides characterization proteomics, valuable reference further analysis difference between caused by fractures those other pathogenic factors. This compendium data insights rich resource better understand

Language: Английский

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Brassinin alleviates cancer cachexia by suppressing diverse inflammatory mechanisms in mice

MedComm, Journal Year: 2024, Volume and Issue: 5(6)

Published: May 28, 2024

Abstract Cancer cachexia is a multifactorial condition that contributes to the death of about 20% cancer patients. It has potential cause weight loss, reduction in muscle mass, and loss fat tissue, significantly lowering quality life. Currently, there are no approved drugs for cachexia. Here, we have explored possible impact brassinin (BSN) on under vitro vivo settings. After differentiation, C2C12 3T3‐L1 cells were incubated with colorectal carcinoma conditioned media or BSN. For preclinical studies, mice injected HT‐29 followed by intraperitoneal administration BSN, adipose tissues evaluated Western blotting hematoxylin eosin staining. BSN effectively suppressed atrophy down‐regulating levels Muscle RING‐finger protein‐1 Atrogin‐1, while also increasing expression myosin heavy chain cachexia‐induced‐C2C12 myotubes. The induction adipogenesis prevented adipocyte cachexia‐induced adipocytes. We noted disrupted interaction between COX‐2 signaling transducer activator transcription 3 (STAT3) promoter, leading down‐regulation STAT3 activation. Moreover, it was found inhibited demonstrated anti‐cachexic effects. Overall, our observations indicate can attenuate through diverse mechanisms.

Language: Английский

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PEI/MMNs@LNA-542 nanoparticles alleviate ICU-acquired weakness through targeted autophagy inhibition and mitochondrial protection

Open Life Sciences, Journal Year: 2024, Volume and Issue: 19(1)

Published: Jan. 1, 2024

Intensive care unit-acquired weakness (ICU-AW) is prevalent in critical care, with limited treatment options. Certain microRNAs, like miR-542, are highly expressed ICU-AW patients. This study investigates the regulatory role and mechanisms of miR-542 explores clinical potential inhibitors. models were established C57BL/6 mice through cecal ligation puncture (CLP) mouse C2C12 myoblasts TNF-α treatment.

Language: Английский

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A scoping review of preclinical intensive care unit-acquired weakness models

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 2, 2024

Background Animal models focusing on neuromuscular outcomes are crucial for understanding the mechanisms of intensive care unit-acquired weakness (ICU-AW) and exploring potential innovative prevention treatment strategies. Aim To analyse evaluate preclinical ICU-AW models. Methods We manually searched five English four Chinese databases from 1 January 2002, to February 2024, reviewed related study references. Full-text publications describing animal muscle atrophy in critical illness were included. Detailed information about model types, species, sex, age, induction methods, outcome measures, drawbacks strengths was extracted each included study. Results A total 3,451 citations initially retrieved, with 84 studies final analysis. The most frequently studied rodents (86.9%), 64.3% which male animals. mostly induced by comprehensive unit (ICU) interventions (38.1%) sepsis (51.2%). Most focused limb muscles (66.7%), diaphragm (21.4%) or both (9.5%). Reported primarily muscular pathological changes (83.3%), electrophysiological examinations (57.1%) grip strength (16.6%). However, details such as mortality data, experimental design, randomisation, blinding, sample size group and/or control inadequately reported. Conclusion Many used ICU-AW, but reporting methodological is often incomplete. Although current ICU can mimic characteristics human there no standard model. Future should develop a enhance reproducibility improve scientific rigor ICU-AW.

Language: Английский

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Negative Effects of Cx43 during Denervated Skeletal Muscle Atrophy Can Be Attenuated by Blueberry Extracts via Regulating Akt Pathway

Journal of Food Biochemistry, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The lack of effective treatments for alleviating irreversible skeletal muscle atrophy caused by peripheral nerve dysfunction is a clinical problem. To identify therapeutic targets, signaling pathways in cells activated denervation have been explored, including the important roles Cx43, kind protein that forms gap junctions, other types pathological process. present study investigated negative role Cx43 aggravated cell degeneration following denervation. blueberry extract was applied orally on mice models, and its protective effect against denervated found wet weight measurement tissue staining observation. Cx43/Akt pathway modulated extract. In conclusion, effects were attenuated regulating Akt pathway.

Language: Английский

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