Animals,
Journal Year:
2024,
Volume and Issue:
14(6), P. 936 - 936
Published: March 19, 2024
During
acute
ruminal
acidosis,
the
manifestation
of
aseptic
polysynovitis
and
lameness
in
cattle
has
been
observed.
Evidence
suggests
that
joint
inflammation
can
be
attributed
to
metabolic
alterations
induced
by
D-lactate
fibroblast-like
synoviocytes
(FLSs).
We
aimed
investigate
whether
andrographolide
could
mitigate
bovine
(bFLSs).
To
assess
this,
bFLSs
were
cultured
presence
or
absence
andrographolide.
evaluated
its
potential
interference
with
expression
proinflammatory
cytokines,
COX-2,
HIF-1α,
LDHA
using
RT-qPCR.
Furthermore,
we
investigated
PI3K/Akt
signaling
IκBα
degradation
through
immunoblotting
flow
cytometry,
respectively.
Our
observations
revealed
reduced
elevation
IL-6,
IL-8,
D-lactate.
Additionally,
demonstrated
NF-κB
pathways
bFLSs.
In
conclusion,
our
findings
suggest
potentially
reverse
inflammatory
effects
changes
bFLSs,
showing
promise
as
a
therapeutic
intervention
for
managing
these
conditions
associated
lameness.
Journal of Orthopaedic Translation,
Journal Year:
2025,
Volume and Issue:
50, P. 56 - 70
Published: Jan. 1, 2025
Osteoarthritis
(OA)
is
a
progressive
degenerative
disease
affected
by
many
factors,
and
there
currently
no
effective
treatment.
In
recent
years,
the
latest
progress
in
metabolomics
OA
research
has
revealed
several
metabolic
pathways
new
specific
metabolites
involved
OA.
Metabolites
play
significant
roles
identification
management
of
This
review
looks
back
on
development
history
this
technology
as
well
its
potential
clinical
applications.
It
summarizes
applications
field
future
directions.
understanding
will
advance
treatment
goals
for
The
offers
possibilities
article
reviews
relationship
between
associated
with
chondrocytes
Selectively
altering
these
three
their
may
hold
great
focal
points
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2025,
Volume and Issue:
13
Published: Feb. 17, 2025
Osteoarthritis
is
one
of
the
most
common
degenerative
joint
diseases,
which
seriously
affects
life
middle-aged
and
elderly
people.
Traditional
treatments
such
as
surgical
treatment
systemic
medication,
often
do
not
achieve
expected
or
optimal
results,
leads
to
severe
trauma
a
variety
side
effects.
Therefore,
there
an
urgent
need
develop
novel
therapeutic
options
overcome
these
problems.
Hydrogels
are
widely
used
in
biomedical
tissue
repairing
platform
for
loading
drugs,
proteins
stem
cells.
In
recent
years,
smart-responsive
hydrogels
have
achieved
excellent
results
drug
delivery
systems
osteoarthritis.
This
review
focuses
on
advances
endogenous
stimuli
(including
enzymes,
pH,
reactive
oxygen
species
temperature,
etc.)
responsive
exogenous
light,
shear,
ultrasound
magnetism,
osteoarthritis
treatment.
Finally,
current
limitations
application
future
prospects
smart
summarized.
Journal of Cellular and Molecular Medicine,
Journal Year:
2025,
Volume and Issue:
29(4)
Published: Feb. 1, 2025
ABSTRACT
Osteoarthritis
presents
a
significant
clinical
challenge
due
to
its
high
prevalence
and
the
resultant
impairment
of
patients'
motor
function.
Osteoarthritic
chondrocytes
are
characterised
by
inflammation
metabolic
disturbances.
Pioglitazone,
an
agonist
peroxisome
proliferator‐activated
receptor
γ
(PPAR‐γ),
has
been
demonstrated
exert
anti‐inflammatory
effects
across
various
diseases.
This
study
aims
investigate
potential
protective
Pioglitazone
on
osteoarthritic
chondrocytes.
An
in
vitro
chondrocyte
model
was
established
utilising
IL‐1β.
The
impact
extracellular
matrix
synthesis
evaluated
through
enzyme‐linked
immunosorbent
assay,
immunofluorescence
staining
Alcian
blue
staining.
affinity
for
PPAR‐γ
investigated
using
molecular
docking
techniques.
Alterations
glycolysis
oxidative
phosphorylation
were
examined
Seahorse
XF
Analyser,
influence
glucose
uptake
mitochondrial
electron
transport
chain
further
analysed.
gavaged
mouse
OA
anterior
cruciate
ligament
transection
evaluate
therapeutic
efficacy
Pioglitazone.
Our
findings
indicate
that
mitigates
osteoarthritis
murine
models
inhibiting
expression
inflammatory
mediators
such
as
TNF‐α,
IL‐6
PGE2,
preventing
degradation
aggrecan
collagen
II.
Furthermore,
significantly
upregulated
transporter
1
stabilised
proton
delivery
PPAR‐γ‐dependent
manner,
thereby
enhancing
uptake,
glycolysis,
phosphorylation.
These
partially
reversed
antagonist
GW9662.
can
confer
chondroprotective
benefits
activating
PPAR‐γ.
Gels,
Journal Year:
2024,
Volume and Issue:
10(7), P. 451 - 451
Published: July 10, 2024
An
electrochemical
sensor
sensitive
to
coenzyme
A
(CoA)
was
designed
using
a
CoA-responsive
polyallylamine-manganese
oxide-polymer
dot
nanogel
coated
on
the
electrode
surface
detect
various
genetic
models
of
osteoarthritis
(OA).
The
responded
abundance
CoA
in
OA,
causing
breakage
MnO
Arthritis Research & Therapy,
Journal Year:
2023,
Volume and Issue:
25(1)
Published: Dec. 4, 2023
Abstract
Purpose
To
identify
the
role
of
gluconeogenesis
in
chondrocytes
osteoarthritis
(OA).
Materials
and
methods
Cartilage
samples
were
collected
from
OA
patients
C57
mice
stained
with
Safranin
O-Fast
Green
to
determine
severity
OA.
Periodic
acid
Schiff
staining
was
used
characterize
contents
polysaccharides
SA-βGal
aging
chondrocytes.
Immunohistochemistry
western
blotting
detect
fructose-bisphosphatase1
(FBP1),
SOX9,
MMP13,
P21,
P16
cartilage
or
chondrocyte.
The
mRNA
levels
fbp1,
mmp13,
sox9,
colX,
acan
analyzed
by
qPCR
evaluate
FBP1
Results
level
reduced
expression
also
reduced.
We
treated
IL-1β
cause
vitro,
then
made
overexpress
plasma.
It
shows
that
alleviated
degeneration
senescence
vitro
it
showed
same
effects
vivo
experiments.
further
understand
mechanism
FBP1,
we
screened
downstream
protein
found
CRB3
significantly
downregulated.
And
confirmed
suppressed
delayed
Conclusions
promoted
polysaccharide
synthesis
regulating
CRB3,
so
is
a
potential
target
treating
