Inflammatory and glycolytic programs underpin a primed blood neutrophil state in patients with pneumonia DOI Creative Commons
Alex R. Schuurman, Joe M. Butler, Erik H.A. Michels

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(7), P. 107181 - 107181

Published: June 19, 2023

Neutrophils are potent immune cells with key antimicrobial functions. Previous in vitro work has shown that neutrophil effector functions mainly fueled by intracellular glycolysis. Little is known about the state of neutrophils still circulation patients during infection. Here, we combined flow cytometry, stimulation assays, transcriptomics, and metabolomics to investigate link between inflammatory metabolic pathways blood community-acquired pneumonia. Patients' neutrophils, relative from age- sex- matched controls, showed increased degranulation upon ex vivo stimulation, portrayed distinct upregulation transcriptional programs. This phenotype was accompanied a high-energy ATP content, transcriptomic glycolysis glycogenolysis. One month after hospital admission, these changes were largely normalized. These data elucidate molecular programs underpin balanced, yet primed

Language: Английский

Microbial Metabolomics: Developments, Applications and Future Prospects DOI

Kiran Kharb,

Sukhminderjit Kaur,

Babita Thakur

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Harnessing Synthetic Biology for Sustainable Industrial Innovation: Advances, Challenges, and Future Direction DOI
Emmanuel Chimeh Ezeako, Barine Innocent Nwiloh,

Malachy Chigozie Odo

et al.

Biochemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 109777 - 109777

Published: May 1, 2025

Language: Английский

Citations

0
Seeding multivariate algorithms for spectral analysis, a data augmentation approach to enhance analytical performance DOI
Mark Keating, Hugh J. Byrne

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126369 - 126369

Published: May 1, 2025

Language: Английский

Citations

0
Enhancement of weak signals by applying a suppression method to high-intense methyl and methylene signals of lipids in NMR spectroscopy DOI Creative Commons
Upendra Singh, Abdul‐Hamid Emwas, Mariusz Jaremko

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(37), P. 26873 - 26883

Published: Jan. 1, 2024

In this study, we applied a method of suppression to suppress the intense peaks methyl and methylene groups allow detection weaker in NMR spectroscopy lipids.

Language: Английский

Citations

3
Inflammatory and glycolytic programs underpin a primed blood neutrophil state in patients with pneumonia DOI Creative Commons
Alex R. Schuurman, Joe M. Butler, Erik H.A. Michels

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(7), P. 107181 - 107181

Published: June 19, 2023

Neutrophils are potent immune cells with key antimicrobial functions. Previous in vitro work has shown that neutrophil effector functions mainly fueled by intracellular glycolysis. Little is known about the state of neutrophils still circulation patients during infection. Here, we combined flow cytometry, stimulation assays, transcriptomics, and metabolomics to investigate link between inflammatory metabolic pathways blood community-acquired pneumonia. Patients' neutrophils, relative from age- sex- matched controls, showed increased degranulation upon ex vivo stimulation, portrayed distinct upregulation transcriptional programs. This phenotype was accompanied a high-energy ATP content, transcriptomic glycolysis glycogenolysis. One month after hospital admission, these changes were largely normalized. These data elucidate molecular programs underpin balanced, yet primed

Language: Английский

Citations

5