Honokiol as an α-glucosidase inhibitor DOI Creative Commons

Hua Zhu,

Xin Zhong

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 19, 2024

Honokiol, a naturally occurring compound from Magnolia obovata Thunb ., has many biological activities, but its anti-α-glucosidase activity is still unclear. Therefore, we determined inhibitory effects against α-glucosidase. Activity assays showed that honokiol was reversible mixed-type inhibitor of α-glucosidase, and IC 50 value 317.11 ± 12.86 μM. Fluorescence results indicated the binding to α-glucosidase caused reduction in activity. 3D fluorescence CD spectra conformational change Docking simulated detailed interactions between including hydrogen hydrophobic bonds. All findings could be used as natural develop agents.

Language: Английский

Rational design of a red-emission fluorescent probe for imaging mitochondrial viscosity and fatty liver tissues DOI
Yuqing Wang,

Deng Yi,

Yaxuan Wang

et al.

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1308, P. 138107 - 138107

Published: March 20, 2024

Language: Английский

Citations

3
A triphenylamine-based fluorescent probe with large Stokes shift for wash-free imaging of lipid droplets and diagnosis of fatty liver DOI

Deng Yi,

Zhiyu Wang, Jie Wang

et al.

New Journal of Chemistry, Journal Year: 2024, Volume and Issue: 48(23), P. 10427 - 10431

Published: Jan. 1, 2024

A D–π–A structural probe was synthesized, which has been validated for distinguishing between fatty liver and normal at the organ levels.

Language: Английский

Citations

3
Inhibition mechanism investigation of quercetagetin as a potential tyrosinase inhibitor DOI Creative Commons

Faliang Liang

Frontiers in Chemistry, Journal Year: 2024, Volume and Issue: 12

Published: June 3, 2024

Tyrosinase is one important rate limiting enzyme in melanin synthesis, directly affecting the synthesis. Quercetagetin active ingredient from marigold. Thence, inhibition effects of quercetagetin against tyrosinase were investigated. The results showed could inhibit activity with IC 50 value 0.19 ± 0.01 mM and type was a reversible mixed-type. Results fluorescence quenching quench static process. CD 3D interaction to change conformation activity. Moreover, docking revealed details quercetagetin’s interactions tyrosinase.

Language: Английский

Citations

3
The effects of cGAS-STING inhibition in liver disease, kidney disease, and cellular senescence DOI Creative Commons
Ling Wang, Zhengwei Zhang, Haichao Zhang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 24, 2024

The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling pathway is one the fundamental mechanisms body's defense, which responds to abnormal presence double-stranded DNA in cytoplasm establish an effective natural immune response. In addition detecting microbial infections, cGAS may be triggered by any cytoplasmic DNA, absent from normal cytoplasm, and only conditions such as senescence mitochondrial stress can lead its leakage cause sterile inflammation. A growing body research has shown that cGAS-STING strongly associated with this study, we reviewed regulatory biological functions through involvement aseptic inflammation liver disease, kidney cellular senescence.

Language: Английский

Citations

3
Lipid droplets-specific fluorescent probe for wash-free imaging and in vivo diagnosis of non-alcoholic fatty liver disease DOI
Yuqing Wang, Jing Yang,

Jie Wang

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(9)

Published: Aug. 12, 2024

Language: Английский

Citations

3
Recent advances in fluorescent probes for fatty liver imaging by detecting lipid droplets DOI
Long He, Hang Li, Yao Tang

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(40), P. 10149 - 10162

Published: Jan. 1, 2024

Fatty liver, a major health problem worldwide, is closely associated with aberrant accumulation and alteration of energy storage organelles, lipid droplets (LDs), in the disease process. Fluorescent probes excellent optical performance, high sensitivity/selectivity real-time monitoring have emerged as an attractive tool for detection LDs used diagnosis fatty liver. In this review, we summarize various based on different response mechanisms to image liver process using excitation imaging modes emission wavelengths, including visible near-infrared, two/three-photon, second near-infrared region. The perspectives barriers reported droplet (LD) future development are also discussed.

Language: Английский

Citations

3
ZNF281 drives hepatocyte senescence in alcoholic liver disease by reducing HK2‐stabilized PINK1/Parkin‐mediated mitophagy DOI Creative Commons
Chunfeng Lu,

Ting Ge,

Yunyun Shao

et al.

Cell Proliferation, Journal Year: 2022, Volume and Issue: 56(3)

Published: Dec. 14, 2022

We investigated the role of zinc-finger protein 281 (ZNF281), a novel molecule, in ethanol-induced hepatocyte senescence and uncovered potential mechanism. Real-time PCR, Western blot, immunofluorescence staining, enzyme-linked immunosorbent assay were performed to explore ZNF281 senescence. expression was upregulated both alcohol-fed mice livers ethanol-treated hepatocytes. Silence hepatocytes using siRNA mitigated ethanol-caused decrease cell viability increased release aspartate aminotransferase, alanine transaminase, lactate dehydrogenase. reduced senescence-associated β-galactosidase-positive cells under ethanol exposure, abolished cycle arrest at G0/G1 phase, diminished secretory phenotype proinflammatory cytokines (IL-1β IL-6) release. At molecular level, deficiency altered profile proteins including p53, p21, p16, high mobility group AT-hook 1, phospho-histone H2A.X telomerase-associated regulatory factors telomerase reverse transcriptase, telomeric repeat binding factor 1 (TRF1), TRF2. knockdown promoted recovery from mitochondrial dysfunction ROS production, which correlated with rescuing HK2-PINK1/Parkin signalling-mediated mitophagy. Mechanistically, directly bound 5'-GGCGGCGGGCGG-3' motif within HK2 promoter region transcriptionally repressed expression. Systematic by adeno-associated virus encoding shRNA protected alcohol feeding-caused injury This study provides as driver during alcoholic liver disease.

Language: Английский

Citations

13
N-p-coumaroyloctopamine ameliorates hepatic glucose metabolism and oxidative stress involved in a PI3K/AKT/GSK3β pathway DOI Creative Commons

Yuechang Huang,

Xingmin Zhang, Qian Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 25, 2024

Type 2 diabetes mellitus is regarded as a chronic metabolic disease characterized by hyperglycemia. Long-term hyperglycemia may result in oxidative stress, damage pancreatic β-cell function and induce insulin resistance. Herein we explored the anti-hypoglycemic effects mechanisms of action N- p -coumaroyloctopamine (N-p-CO) vitro vivo . N-p-CO exhibited high antioxidant activity, indicated increased activity SOD, GSH GSH-Px HL-7702 cells induced both glucose (HG) palmitic acid (PA). treatment significantly augmented uptake glycogen synthesis HG/PA-treated cells. Moreover, administration diabetic mice high-fat diet (HFD) streptozotocin (STZ) not only levels GSH-PX, SOD GSH, but also dramatically alleviated hepatic metabolism dose-dependent manner. More importantly, upregulated expressions PI3K, AKT GSK3β proteins HG/PA-induced HFD/STZ-induced mice. These findings clearly suggest that exerts anti-oxidant effects, most probably via regulation PI3K/AKT/GSK3β signaling pathway. Thus, have potentials new candidate for prevention diabetes.

Language: Английский

Citations

2
Zn(II)–curcumin prevents cadmium-aggravated diabetic nephropathy by regulating gut microbiota and zinc homeostasis DOI Creative Commons
Wenjia Sun,

Xueting Mei,

Jiasheng Wang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 5, 2024

Background: Diabetic nephropathy (DN) is known as the most common complication of diabetes, resulting from a complex inheritance-environment interaction without effective clinical treatments. Herein, we revealed protective effects and mechanisms Zn(II)-curcumin, curcumin derivative, against streptozotocin-induced DN in rats presence or absence cadmium exposure. Methods: The present study focused on investigating therapy Zn(II)-curcumin cadmium-aggravated by regulating gut microbiota, metabolism, inflammation zinc homeostasis based pathological changes, TLR4/NF-κB signaling pathway, inductively coupled plasma-mass spectrometry (ICP-MS), 16S rRNA gene sequencing gas chromatography-mass spectrometer (GC-MS). Results: We found significantly mitigated phenotypes diabetic nephropathy, indicated remission renal dysfunction, dyshomeostasis streptozotocin-treated exposed to cadmium. Administration alleviated dysbiosis microbiota changes serum metabolite profiles treated with streptozotocin combination Notably, fecal microbial transplantation identified ability regulate function, was partly dependent microbiota. Conclusion: These findings that reshaping homeostasis, which provided unique insights into treatment prevention nephropathy.

Language: Английский

Citations

2
The crucial roles and research advances of cGAS‑STING pathway in liver diseases DOI Creative Commons

Xiaoqian Zhang,

Bin He, Juan Lu

et al.

Annals of Medicine, Journal Year: 2024, Volume and Issue: 56(1)

Published: Aug. 25, 2024

Inflammation responses have identified as a key mediator of in various liver diseases with high morbidity and mortality. cGAS-STING signalling is essential innate immunity since it triggers release type I interferons proinflammatory cytokines. The potential connection between pathway inflammatory has recently been reported widely. In our review, the impact on inflammation regulatory mechanism are summarized. Furthermore, many inhibitors promising agents to cure also explored detail. A comprehensive knowledge molecular mechanisms vital for exploring novel treatments providing recommendations perspectives future utilization.

Language: Английский

Citations

2