Subchronic toxicity evaluation of Huobahuagen extract and plasma metabolic profiling analysis combined with conventional pathology methods DOI
Chengyan Long, Peilin He,

Ruxia Tu

et al.

Journal of Applied Toxicology, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Abstract Huobahua, namely, Tripterygium hypoglaucum (Levl.) Hutch, known as a traditional Chinese herbal medicine, especially its underground parts, has been widely developed into several agents for the treatment of rheumatoid arthritis and other autoimmune diseases. It sparked wide public concern about safety, such multi‐organ toxicity. However, toxic characteristics damage mechanism Huobahuagen extract (HBHGE) remain unclear. In present study, subchronic oral toxicity study HBHGE (10.0 g crude drug/kg/day 12 weeks) was performed in male rats. Hematological, serum biochemical, histopathological parameters, urinalysis, plasma metabolic profiling were assessed. The single‐dose results related to exhibited obvious testis epididymis Furthermore, metabolomics analysis suggested that series disorders induced by administration HBHGE, mainly focusing on amino acid (glutamate, phenylalanine, tryptophan) metabolisms, pyrimidine metabolism, glutathione steroid hormone biosynthesis. Moreover, it appeared testosterone rats treated with weeks, decreased significantly, susceptible effects HBHGE. Taken together, conventional pathology preliminarily exploring underlying can provide useful information reduction risks from new insights development detoxification preparations.

Language: Английский

Kidney-tonifying blood-activating decoction delays ventricular remodeling in rats with chronic heart failure by regulating gut microbiota and metabolites and p38 mitogen-activated protein kinase/p65 nuclear factor kappa-B/aquaporin-4 signaling pathway DOI
Rui‐Hua Xu, Yanping Bi,

Xiaoteng He

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 330, P. 118110 - 118110

Published: April 4, 2024

Language: Английский

Citations

6
The mechanisms of tripterygium glycosides-induced reproductive toxicity and detoxification strategies DOI

Zechen Niu,

Huanhuan Zhang,

Chunzhou Cai

et al.

Reproductive Toxicology, Journal Year: 2025, Volume and Issue: 132, P. 108830 - 108830

Published: Jan. 6, 2025

Language: Английский

Citations

0
PLIN1 suppresses glioma progression through regulating lipid metabolism DOI Creative Commons

Kui Luo,

Kai Zhuang, Hao Wu

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 27, 2025

Abstract Glioma is a common and destructive brain tumor, which highly heterogeneous with poor prognosis. Developing diagnostic prognostic markers to identify treat glioma early would significantly improve the therapeutic outcomes. Here, we conducted RNA next-generation sequencing 33 samples 15 normal samples. We found Perilipin 1 (PLIN1) downregulated in correlated poorer outcome. Subsequent experiments revealed that up regulation of PLIN1 led repressed cell growth invasion glioma. Moreover, overexpression increased lipid accumulation cells, increasing expression biosynthesis related genes decreasing lipolysis genes. Mechanically, PI3K/AKT axis could regulate levels glioma, inhibition activity increase In conclusion, dysregulation downregulation following tumor proliferation, metabolism reprogramming

Language: Английский

Citations

0
Zinc Ameliorates Tripterygium Glycosides–Induced Reproductive Impairment in Male Rats by Regulating Zinc Homeostasis and Expression of Oxidative Stress–Related Genes DOI
Jing Ma,

He Tan,

Jiajie Bi

et al.

Biological Trace Element Research, Journal Year: 2023, Volume and Issue: 202(5), P. 2111 - 2123

Published: Aug. 23, 2023

Language: Английский

Citations

7
Subchronic toxicity evaluation of Huobahuagen extract and plasma metabolic profiling analysis combined with conventional pathology methods DOI
Chengyan Long, Peilin He,

Ruxia Tu

et al.

Journal of Applied Toxicology, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Abstract Huobahua, namely, Tripterygium hypoglaucum (Levl.) Hutch, known as a traditional Chinese herbal medicine, especially its underground parts, has been widely developed into several agents for the treatment of rheumatoid arthritis and other autoimmune diseases. It sparked wide public concern about safety, such multi‐organ toxicity. However, toxic characteristics damage mechanism Huobahuagen extract (HBHGE) remain unclear. In present study, subchronic oral toxicity study HBHGE (10.0 g crude drug/kg/day 12 weeks) was performed in male rats. Hematological, serum biochemical, histopathological parameters, urinalysis, plasma metabolic profiling were assessed. The single‐dose results related to exhibited obvious testis epididymis Furthermore, metabolomics analysis suggested that series disorders induced by administration HBHGE, mainly focusing on amino acid (glutamate, phenylalanine, tryptophan) metabolisms, pyrimidine metabolism, glutathione steroid hormone biosynthesis. Moreover, it appeared testosterone rats treated with weeks, decreased significantly, susceptible effects HBHGE. Taken together, conventional pathology preliminarily exploring underlying can provide useful information reduction risks from new insights development detoxification preparations.

Language: Английский

Citations

0