Photodiagnosis and Photodynamic Therapy,
Journal Year:
2024,
Volume and Issue:
49, P. 104319 - 104319
Published: Aug. 23, 2024
Photodynamic
therapy
(PDT)
is
a
promising
and
innovative
approach
for
treating
tumors.
The
synergistic
effect
of
PDT
chemotherapy
can
enhance
the
anti-tumor
efficacy
by
leveraging
their
complementing
benefits.
In
this
study,
we
created
lipid
vesicles
to
deliver
photosensitizer
(chlorin
e6,
Ce6)
Regorafenib
into
tumors
purpose
examining
effectiveness
mechanism
Lipo-Ce6@Rego-PDT
(LCR-P)
on
Hepatocellular
carcinoma
(HCC)
both
in
vitro
vivo.
We
found
that
cytotoxicity
HCC
caused
LCR-P
was
significantly
stronger
than
Lipo-Ce6-PDT
(LC-P).
Cellular
ROS
production
group
approximately
higher
LC-P
group,
inhibited
phosphorylation
JNK,
ERK,
P38
Furthermore,
downregulated
expression
Bcl-2
upregulated
Bax
cleaved
caspase-3
Compared
with
LC-P,
increased
cell
apoptosis
rate.
body
weight
HE
staining
normal
organs
primarily
indicated
safety
combined
strategy.
These
results
indicate
combination
Lipo-Ce6
efficiency
exhibits
good
biosafety.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
14
Published: Jan. 11, 2024
Hepatocellular
carcinoma
(HCC)
is
the
most
common
type
of
liver
cancer,
accounting
for
~90%
total
cases.
DnaJ
heat
shock
protein
family
member
C8
(DNAJC8),
belonging
to
40
(HSP40)
family,
known
regulate
cancer
biology
function.
However,
role
DNAJC8
on
HCC
development
remains
unknown.
Authorea (Authorea),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 30, 2024
Ten
novel
isocoumarins,
including
four
pairs
of
enantiomers,
were
isolated
from
Artemisia
dubia
var.
subdigitata
(Asteraceae).
Com-pounds
1,
2
and
3a/3b
possessed
a
unique
6/6/6-tricyclic
system
comprising
an
unusual
1-(2-methylcyclohexyl)
propan-1-one
moiety
fused
with
isocoumarin
core
skeleton.
Compounds
4a/4b
characterized
as
unexpected
2,5-dimethylcyclohexan-1-one
scaffold,
compounds
5a/5b
6a/6b
rare
1,2-seco-isocoumarin.
Their
structures
absolute
configurations
elucidated
through
spectroscopic
data,
X-ray
crystallography,
ECD
NMR
calculations
DP4+
analyses.
Plausible
biosynthetic
pathways
proposed
the
naturally
occurring
isocoumarin.
Network
pharmacological
analysis
suggested
that
targets
compound
1
significantly
enriched
in
cell
cycle
PI3K-Akt
signaling
pathway.
The
molecular
docking
revealed
had
high
binding
affinity
CDK2
(total
score:
6.8717).
Furthermore,
exhibited
inhibitory
activity
on
three
human
hepa-toma
lines,
ratios
85.1%
84.5%
(HepG2),
88.2%
87.3%
(Huh7),
69.2%
69.1%
(SK-Hep-1)
at
200
μM,
respectively.
Anti-Cancer Agents in Medicinal Chemistry,
Journal Year:
2024,
Volume and Issue:
24(19), P. 1431 - 1441
Published: Aug. 12, 2024
Introduction:
Sorafenib
(Sor)
is
the
first-line
treatment
option
in
clinics
for
treating
advanced
unresectable
hepatocellular
carcinoma
(HCC).
However,
acquired
chemoresistance
and
adverse
side
effects
associated
with
Sor
monotherapy
limit
its
clinical
benefits.
We
have
previously
reported
exceptional
anti-HCC
potential
of
uttroside
B
(Utt-B),
a
furostanol
saponin
isolated
our
lab
from
Solanum
nigrum
Linn.
leaves.
The
current
study
has
evaluated
supremacy
combinatorial
regimen
Utt-B
over
monotherapy.
Methods:
MTT
assay
was
used
In
vitro
cytotoxicity
studies.
A
clonogenic
conducted
to
assess
anti-proliferative
effect
combination.
Annexin
V/PI
staining,
confocal
microscopy,
FACS
cell
cycle
analysis,
Western
blotting
experiments
were
performed
validate
pro-apoptotic
combination
HepG2
Huh7
lines.
Pharmacological
safety
evaluation
Swiss
albino
mice.
Results:
Our
results
indicate
that
augments
Sor-induced
cells.
inhibits
proliferation
liver
cancer
cells
by
inducing
apoptosis
through
activation
caspases
7
3,
leading
PARP
cleavage.
Furthermore,
does
not
induce
any
acute
toxicity
vivo,
even
at
dose
five
times
effective
therapeutic
dose.
Conclusion:
highlight
as
an
chemosensitizer,
which
can
augment
efficacy
against
HCC
circumvent
toxic
effects.
Moreover,
this
first
only
report
date
on
chemosensitizing
demonstrates
pharmacological
novel
involving
combating
HCC.
Photodiagnosis and Photodynamic Therapy,
Journal Year:
2024,
Volume and Issue:
49, P. 104319 - 104319
Published: Aug. 23, 2024
Photodynamic
therapy
(PDT)
is
a
promising
and
innovative
approach
for
treating
tumors.
The
synergistic
effect
of
PDT
chemotherapy
can
enhance
the
anti-tumor
efficacy
by
leveraging
their
complementing
benefits.
In
this
study,
we
created
lipid
vesicles
to
deliver
photosensitizer
(chlorin
e6,
Ce6)
Regorafenib
into
tumors
purpose
examining
effectiveness
mechanism
Lipo-Ce6@Rego-PDT
(LCR-P)
on
Hepatocellular
carcinoma
(HCC)
both
in
vitro
vivo.
We
found
that
cytotoxicity
HCC
caused
LCR-P
was
significantly
stronger
than
Lipo-Ce6-PDT
(LC-P).
Cellular
ROS
production
group
approximately
higher
LC-P
group,
inhibited
phosphorylation
JNK,
ERK,
P38
Furthermore,
downregulated
expression
Bcl-2
upregulated
Bax
cleaved
caspase-3
Compared
with
LC-P,
increased
cell
apoptosis
rate.
body
weight
HE
staining
normal
organs
primarily
indicated
safety
combined
strategy.
These
results
indicate
combination
Lipo-Ce6
efficiency
exhibits
good
biosafety.
