DEVELOPING NEW DUAL-ACTION ANTIVIRAL/ANTI-INFLAMMATORY SMALL MOLECULES FOR COVID-19 TREATMENT USING IN SILICO AND IN-VITRO APPROACHES DOI Creative Commons
Vladimir V. Ivanov, Anton B. Zakharov, Dmytro Anokhin

et al.

Published: Nov. 11, 2024

Abstract This study aims to develop new molecular structures as potential therapeutic agents against COVID-19, utilizing both in silico and vitro studies. Potential targets of cepharanthine (CEP) COVID-19 reveal its underlying mechanism action were evaluated using screening experiments. A library molecules was docked into the receptor binding domain SARS-CoV-2 spike glycoprotein complex with receptor, human ACE2, identify promising compounds. Receptor-oriented docking performed most likely macromolecular targets, aimed at inhibiting key viral replication pathways reducing inflammatory processes damaged tissues. The hit showed inhibition Mpro PLpro proteases SARS-CoV-2, which are involved replication. They also a inhibitory effect on Janus kinase (Jak3), mediates intracellular signaling responsible for processes. examined effects selected generation superoxide anions release elastase activated neutrophils, factors that exacerbate tissue inflammation worsen clinical manifestations COVID-19. It demonstrated 2-((5-((4-isopropylphenyl)sulfonyl)-6-oxo-1,6-dihydropyrimidin-2-yl)thio)-N-(3-methoxyphenyl)acetamide ( Hit15 ) inhibited virus infection by 43.0% 10 μ M pseudovirus assay suppressed fMLF/CB-induced anion neutrophils IC 50 values 1.43 1.28 M, respectively. activity coronavirus can be further developed agent. Graphical

Language: Английский

Highly sensitive detection of multiple antiviral drugs using graphitized hydroxylated multi-walled carbon nanotubes/ionic liquids-based electrochemical sensors DOI
Zhipeng Zhang,

Huizi Zheng,

Ying Liu

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 249, P. 118466 - 118466

Published: Feb. 12, 2024

Language: Английский

Citations

10
Protective Role of Cepharanthine Against Equid Herpesvirus Type 8 Through AMPK and Nrf2/HO-1 Pathway Activation DOI Creative Commons
Shuwen Li, Liangliang Li, Yueqi Sun

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(11), P. 1765 - 1765

Published: Nov. 12, 2024

Equid herpesvirus type 8 (EqHV-8) is known to cause respiratory disease and miscarriage in horses donkeys, which a major problem for the equine farming industry. However, there are currently limited vaccines or drugs available effectively treat EqHV-8 infection. Therefore, it crucial develop new antiviral approaches prevent potential pandemics caused by EqHV-8. This study evaluates antioxidant effects of cepharanthine against employing both vitro assays vivo mouse models assess its therapeutic efficacy. To effectiveness EqHV-8, we conducted experiments using NBL-6 RK-13 cells. Additionally, developed model validate cepharanthine’s In our experiments, assessed ability inhibit infection Our results demonstrated that has dose-dependent inhibitory effect, indicating possesses anti-EqHV-8 properties at cellular level. Moreover, investigated mechanism through exerts protective effects. It was observed reduces oxidative stress induced activating AMPK Nrf2/HO-1 signaling pathways. Furthermore, when administered infected mice, significantly improved lung tissue pathology reduced stress. The findings presented herein collectively highlight as promising candidate combating infections.

Language: Английский

Citations

2
A Reappraisal of the Antiviral Properties of and Immune Regulation through Dietary Phytochemicals DOI
Mony Thakur, Mona Singh,

Sandeep Kumar

et al.

ACS Pharmacology & Translational Science, Journal Year: 2023, Volume and Issue: 6(11), P. 1600 - 1615

Published: Oct. 10, 2023

In the present era of COVID-19 pandemic, viral infections remain a major cause morbidity and mortality worldwide. this day age, are rampant spreading rapidly. Among most aggressive ebola, AIDS (acquired immunodeficiency syndrome), influenza, SARS (severe acute respiratory syndrome). Even though there few treatment options for diseases, antiviral therapies ineffective owing to frequent mutations, development more strains, drug resistance, possible side effects. Traditionally, herbal remedies have been used by healers, including dietary medicinal purposes. Many clinical scientific studies demonstrated therapeutic potential plant-derived natural compounds. Because unsafe practices like blood transfusions organ transplants from infected patients, medical supply contamination. Our cannot achieve sterile immunity, we yet find cure these pernicious infections. Herbs shown improve efficacy against wide variety diseases because their high concentration immunomodulatory phytochemicals (both immunoinhibitory anti-inflammatory). Combined with biotechnology, folk medicine system can lead novel drugs therapies. Review, will summarize some selected bioactive compounds probable mechanisms actions, focusing on immunological axis

Language: Английский

Citations

5
Genome assembly of Stephania longa provides insight into cepharanthine biosynthesis DOI Creative Commons
Huiying Shang, Yuan Lü, Lulu Xun

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 5, 2024

, a medicinal plant renowned for producing cepharanthine, has gained significance due to the compound's notable antiviral properties against SARS-CoV-2. However, comprehensive genetic understanding of S. longa been lacking. This study aimed develop high-quality, chromosome-level genome assembly uncover intricacies and evolutionary narrative this species. By integrating genomic data with metabolomic transcriptomic analyses, we sought identify key genes involved in cepharanthine biosynthesis.

Language: Английский

Citations

1
Repurposing existing drugs for the treatment ofCOVID-19/SARS-CoV-2: A review of pharmacological effects and mechanism of action DOI Creative Commons
Yutong Liang,

Xiaoxiao Quan,

Ruolan Gu

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e35988 - e35988

Published: Aug. 1, 2024

Language: Английский

Citations

0
DEVELOPING NEW DUAL-ACTION ANTIVIRAL/ANTI-INFLAMMATORY SMALL MOLECULES FOR COVID-19 TREATMENT USING IN SILICO AND IN-VITRO APPROACHES DOI Creative Commons
Vladimir V. Ivanov, Anton B. Zakharov, Dmytro Anokhin

et al.

Published: Nov. 11, 2024

Abstract This study aims to develop new molecular structures as potential therapeutic agents against COVID-19, utilizing both in silico and vitro studies. Potential targets of cepharanthine (CEP) COVID-19 reveal its underlying mechanism action were evaluated using screening experiments. A library molecules was docked into the receptor binding domain SARS-CoV-2 spike glycoprotein complex with receptor, human ACE2, identify promising compounds. Receptor-oriented docking performed most likely macromolecular targets, aimed at inhibiting key viral replication pathways reducing inflammatory processes damaged tissues. The hit showed inhibition Mpro PLpro proteases SARS-CoV-2, which are involved replication. They also a inhibitory effect on Janus kinase (Jak3), mediates intracellular signaling responsible for processes. examined effects selected generation superoxide anions release elastase activated neutrophils, factors that exacerbate tissue inflammation worsen clinical manifestations COVID-19. It demonstrated 2-((5-((4-isopropylphenyl)sulfonyl)-6-oxo-1,6-dihydropyrimidin-2-yl)thio)-N-(3-methoxyphenyl)acetamide ( Hit15 ) inhibited virus infection by 43.0% 10 μ M pseudovirus assay suppressed fMLF/CB-induced anion neutrophils IC 50 values 1.43 1.28 M, respectively. activity coronavirus can be further developed agent. Graphical

Language: Английский

Citations

0