Propofol Inhibits Oxidative Stress-Induced Neuronal Cell Ferroptosis and Promotes Synaptic Plasticity via the AMPK/ SIRT1/PGC-1α Axis

Indian Journal of Pharmaceutical Education and Research, Journal Year: 2025, Volume and Issue: 59(1), P. 185 - 195

Published: Jan. 6, 2025

Aim/Background Propofol, recognized for its quick and effective action as a hypnotic anesthetic, is frequently utilized in clinical practice. Our research intended to investigate the mechanism by which propofol inhibits neuronal ferroptosis promotes synaptic plasticity via mitochondrial energy regulation. Materials Methods Mouse Hippocampal Neurons (HT22) cells were treated with RAS-Selective Lethal 3 (RSL3) followed Reactive Oxygen species (ROS) detection. Expression levels of regulation analyzed. HT22 Sirtuin 1 (SIRT1) inhibitor before treatment. Level ROS, Fe2+ genes expression measured. Results In RSL3-Low+propofol cohort, exhibiting stark contrast both mock group RSL3-High+propofol group, administration notably attenuated Cyclooxygenase 2 (COX-2) Long-chain-fatty-acid-CoA Ligase 4 (ACSL4), while concurrently enhancing Glutathione Peroxidase (GPX4), Solute Carrier Family 7 Member 11 (SLC7A11), Nuclear Factor-like (NRF2), Ferritin Heavy chain (FTH1), Adenosine 5'-Monophosphate (AMP)-Activated Protein Kinase (AMPK), SIRT1 PPARγ Coactivator-1 α (PGC-1α). ROS substantially greater Selisistat+propofol than whereas PGC-1α considerably less comparison groups. Conversely, showed significantly higher AMPK, SIRT1, PGC-1α, Synapsin-1 (SYN1) PSD-95 compared (p <0.05). Conclusion Propofol oxidative stress-induced cell AMPK/ SIRT1/PGC-1α axis.

Language: Английский

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Propofol alleviates ropivacaine-induced neurotoxicity through the MALAT1/EZH2/BDNF axis

Molecular & Cellular Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

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Anesthesia-mediated neuroinflammatory sequelae in post operative cognitive dysfunction: mechanisms and therapeutic implications

Frontiers in Anesthesiology, Journal Year: 2024, Volume and Issue: 3

Published: Feb. 27, 2024

Post-operative cognitive dysfunction (POCD) is an iatrogenic decline with unclear etiology. While current hypotheses include surgical and pharmacological-induced neuroinflammatory mechanisms, the growing prevalence, especially amongst geriatric population, emphasizes ambiguity of dysfunction. Recent studies have highlighted potential role general regional anesthesia in pathogenesis POCD; these pharmacological effects been demonstrated to disrupt blood-brain barrier integrity, influence microglial polarization, linked worsening prognoses decline. Moreover, mechanical stress from intervention reperfusion injury may exacerbate generation reactive oxygen species (ROS), thereby increasing oxidative brain synergistically disruptions. In previous studies, factors for variable incidence various risk explored. this review, we examine local, regional, on molecular cellular glial response, along its intercellular interactions previously reported clinical outcomes.

Language: Английский

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Hidden role of microglia during neurodegenerative disorders and neurocritical care: A mitochondrial perspective

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 142, P. 113024 - 113024

Published: Aug. 31, 2024

Language: Английский

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Carbon Nanodots Inhibit Tumor Necrosis Factor-α-Induced Endothelial Inflammation through Scavenging Hydrogen Peroxide and Upregulating Antioxidant Gene Expression in EA.hy926 Endothelial Cells

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 224 - 224

Published: Feb. 10, 2024

Carbon nanodots (CNDs) are a new type of nanomaterial with size less than 10 nanometers and excellent biocompatibility, widely used in fields such as biological imaging, transmission, diagnosis, drug delivery. However, its potential mechanism to mediate endothelial inflammation have yet be explored. Here, we report that the uptake CNDs by EA.hy926 cells is both time dose dependent. The concentration this experiment was found not affect cell viability. TNF-α known biomarker vascular inflammation. Cells treated for 24 h significantly inhibited (0.5 ng/mL)-induced expression intracellular adhesion molecule 1 (ICAM-1) interleukin 8 (IL-8). ICAM-1 IL-8 two key molecules responsible activation firm monocytes activated initiation atherosclerosis. ROS, hydrogen peroxide, play an important role TNF-α-induced Interestingly, effectively scavenged H2O2 dose-dependent manner. treatment also increased activity antioxidant enzyme NQO1 indicating properties CNDs. These results suggest anti-inflammatory effects may due direct scavenging indirect upregulation cells. In conclusion, CND can inhibit inflammation, possibly

Language: Английский

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Acute hyperglycemia exacerbates neuroinflammation and cognitive impairment in sepsis-associated encephalopathy by mediating the ChREBP/HIF-1α pathway

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Nov. 14, 2024

Delirium is a prominent symptom of sepsis-associated encephalopathy (SAE) and highly prevalent in septic patients hospitalized the intensive care unit, being closely connected with raised mortality rates. Acute hyperglycemia (AH) has been recognized as separate risk factor for delirium worse prognosis critically sick patients. Nevertheless, exact contribution AH to advancement SAE still unknown. This research retrospectively evaluated connection between blood glucose levels (BGLs) incidence death rates ICU tertiary comprehensive hospital. In addition, rat model was induced through cecal ligation puncture (CLP), after which continuous infusion promptly initiated via central venous catheter post-surgery evaluate potential implications on SAE. Next, rats were assigned four groups based target BGLs: high group (HG, ≥ 300 mg/dL), moderate (MG, 200–300 normal (NG, < 200 insulin-treated (HI, mg/dL) receiving recombinant human insulin treatment (0.1 IU/kg/min). The sham (SG) received an equivalent volume saline denoted NG group. effects neuroinflammation cognitive function using behavioral tests, histopathological examination, TUNEL staining, ELISA, Western blot. microglial activation metabolism following lipopolysaccharide (LPS, 1 μg/mL) exposure assessed CCK8 assay, qRT-PCR, blot, ELISA. Our findings revealed that during sepsis assisted predicting occurrence. systemic inflammatory cytokines rats, promoting neuronal apoptosis, blood–brain barrier disruption, impairment. both vivo vitro, elevated challenge increased ChREBP, HIF-1α, glycolytic enzymes, expressions microglia CLP or LPS. These results collectively suggest can exacerbate by enhancing glycolysis under conditions, potentially mediated upregulation ChREBP/HIF-1α signaling pathway.

Language: Английский

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Huanshaodan regulates microglial glucose metabolism reprogramming to alleviate neuroinflammation in AD mice through mTOR/HIF-1α signaling pathway

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 26, 2024

Abnormal glucose metabolism in microglial is closely associated with Alzheimer's disease (AD). Reprogramming of centered on regulating the way which metabolize to alter function. Therefore, reprogramming considered as a therapeutic strategy for AD. Huanshaodan (HSD) Chinese herbal compound shows significant efficacy treating AD, however, precise mechanism by HSD treats AD remains unclear. This study aim investigate whether exerts anti-AD effects metabolic through mTOR/HIF-1α signaling pathway. SAMP8 mice and BV2 cells were used explore alleviative effect molecular

Language: Английский

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DANOS ENCEFÁLICOS COMO RESULTADO DA ATIVAÇÃO DA MICROGLIA NA ENCEFALOPATIA ASSOCIADA A SEPSE

Revista Foco, Journal Year: 2024, Volume and Issue: 17(7), P. e5630 - e5630

Published: July 31, 2024

A revisão objetiva compreender o papel central da micróglia como mediador das disfunções cognitivas resultantes encefalopatia associada à sepse (SAE) e traçar relações com os potenciais mecanismos envolvidos nesse processo patológico. SAE é uma importante patologia, muito comum no cenário de unidades terapia intensiva. Tal condição possui relação intrínseca a população celular denominada micróglia, essa que ainda não está fortemente consolidada na literatura científica, permanece algumas lacunas. partir disso, foi realizado um estudo cunho bibliográfico descritivo, forma integrativa literatura. O delineamento dos dados obtido bases Scielo, PubMed BVS, sendo incluídos artigos últimos 5 anos. Os estudos encontrados foram organizados selecionados do método verificação PRISMA, por fim, textos reunidos analisados para dissertação revisão. pesquisa permitiu elucidação inúmeros ativação microglial suas consequentes cascatas reações, bem como, entendimento pontos-chave vias podem ser utilizados reguladores inflamação, neuroprotetores achados contribuir realização pesquisas futuras indiquem tratamentos eficazes danos cognitivos neurais resultados microglial.

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Review on the Role of Mitochondrial Dysfunction in Septic Encephalopathy

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Language: Английский

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Comprehensive review of perioperative factors influencing ferroptosis

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117375 - 117375

Published: Sept. 14, 2024

Language: Английский

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