Phospholipase Cδ-4 (PLCδ4) Acts as a Nuclear Player to Influence Cyclin B Expression in the Embryonal Rhabdomyosarcoma Cell Lines RD and A204

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1180 - 1180

Published: Sept. 20, 2024

Rhabdomyosarcoma (RMS), the most common form of sarcoma typical pediatric age, arises from malignant transformation mesenchymal precursors that fail to differentiate into skeletal muscle cells. Here, we investigated whether protein phospholipase C δ4 (PLCδ4), a member PLC family involved in proliferation and senescence mechanisms stromal stem cells, may play role RMS. Our molecular morpho-functional data reveal PLCδ4 is highly expressed fusion-negative, p53-positive, SMARCB1 heterozygous mutated embryonal RMS (ERMS) cell line A204, while it poorly ERMS lines RD (fusion-negative, MYC amplification, N-RAS (Q61H), homozygous p53) Hs729 (homozygous alveolar rhabdosarcoma (ARMS) SJCRH30 (RH30; fusion positive, RARA, polyheterozygous p53). To characterize PLCδ4, was stably transfected with wild-type (RD/PLCδ4). Overexpressed mainly localized nucleus cells contributed phosphorylation PRAS40 (T246), Chk2(T68), WNK1(T60), Akt 1/273 (S473), as revealed by proteome profiler array analysis. Overexpression enhanced cyclin B1 expression resulted G2/M-phase cycle arrest. In contrast, siRNA-mediated knockdown A204 reduced expression. study identifies novel for nuclear regulator via Akt-dependent phosphorylation. The modulation its downstream targets could represent crucial signaling pathway block proliferation.

Language: Английский

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Global, Regional, and National Burden of Soft Tissue and Other Extraosseous Sarcomas in Adolescents and Young Adults,1990-2021: A Systematic Analysis for the Global Burden of Disease Study 2021

Published: Jan. 1, 2024

Language: Английский

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Malignant sarcomatous transformation of calvarial fibrous dysplasia: illustrative case

Journal of Neurosurgery Case Lessons, Journal Year: 2024, Volume and Issue: 8(20)

Published: Nov. 11, 2024

BACKGROUND Fibrous dysplasia (FD) is a nonheritable genetic disorder characterized by abnormal osteogenesis, resulting in benign bone lesions one or multiple bones. Despite their predominantly nature, these can transform into malignant neoplasms, pain, swelling, disfigurement, and even death. The authors report case of sarcomatous transformation an adult patient with history craniofacial FD. OBSERVATIONS A 61-year-old male systemic FD presented rapidly enlarging cranial mass that had recently started becoming painful to touch. Magnetic resonance imaging the brain displayed extensive changes throughout calvarium large dysplastic right parietal extension underlying epidural space, severe effect. subsequently underwent radical resection complex soft tissue reconstruction. pathology confirmed high-grade undifferentiated pleomorphic sarcoma, for which adjuvant chemotherapy regimen. LESSONS Malignant most often results formation osteosarcoma maxilla, temporal sphenoid bones also being common sites such transformations. Although treatment begins resection, efficacy radiation remains controversial. https://thejns.org/doi/10.3171/CASE24537

Language: Английский

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Comprehensive study of gene fusions in sarcomas

Investigational New Drugs, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Language: Английский

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