Luteolin Regulating Synthesis and Catabolism of Osteoarthritis Chondrocytes Via Activating Autophagy DOI

Fuen Liu,

Zhenfei Ding,

Shuang Li

et al.

Published: Jan. 1, 2023

Osteoarthritis (OA) is a common bone and joint disease characterized by degeneration. The imbalance between the synthesis breakdown of chondrocytes major contributing factor to OA. A potential treatment for OA could involve targeting degenerative changes in cartilage tissue degradation. Previous studies have shown close relationship autophagy regulation chondrocyte breakdown. Activation has been found alleviate degeneration tissue. Currently, primary focus osteoarthritis symptom relief, as there no effective medication halt progression. In previous studies, luteolin, flavonoid Chinese herbal medicine, activate reduce expression MMP1 ADAMTS-5. this study, an vitro model was created using stimulated IL-1β, different concentrations luteolin were used treatment. Treatment with significantly increased levels factors Aggrecan Collagen II, while decreasing decomposition MMP-13 Furthermore, when inhibited Chloroquine, imbalances anabolic catabolic activities induced IL-1β reversed. vivo knee medial meniscal instability (DMM), administered therapeutic schedule. After 12 weeks, tissues mice collected analysis. Immunofluorescence immunohistochemical staining showed decrease level P62 increase Beclin-1 tissues. Additionally, wear joints relieved safranin O fast green staining. findings our study highlight significant role effectively reversing IL-1β. Our results strongly indicate that be developed novel agent osteoarthritis, offering promising prospects future drug development.

Language: Английский

Luteolin: A promising multifunctional natural flavonoid for human diseases DOI Creative Commons
Mingtao Zhu, Yanping Sun, Yang Su

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: 38(7), P. 3417 - 3443

Published: April 26, 2024

Abstract Natural products are closely associated with human health. Luteolin (LUT), a flavonoid polyphenolic compound, is widely found in fruits, vegetables, flowers, and herbs. It noteworthy that LUT exhibits variety of beneficial pharmacological properties holds significant potential for clinical applications, particularly antitumor, anti‐convulsion, diabetes control, anti‐inflammatory, neuroprotection, anti‐oxidation, anti‐cardiovascular, other aspects. The mechanism action has been partially elucidated, including the mediation NF‐κB, toll‐like receptor, MAPK, Wnt/β‐catenin, PI3K/Akt, AMPK/mTOR, Nrf‐2, among others. review aimed to comprehensively consolidate essential information on natural sources, effects, therapeutic preventive potential, as well mechanisms LUT. objective establish theoretical basis continued development application

Language: Английский

Citations

28
Luteolin as a potential therapeutic candidate for lung cancer: Emerging preclinical evidence DOI Open Access
Jin Zhang, Yue Ma

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116909 - 116909

Published: June 8, 2024

Lung cancer is a prevalent malignant tumor and leading cause of cancer-related fatalities globally. However, current treatments all have limitations. Therefore, there an urgent need to identify readily available therapeutic agent counteract lung development progression. Luteolin flavonoid derived from vegetables herbs that possesses preventive effects on various cancers. With the goal providing new directions for treatment cancer, we review here recent findings luteolin so as provide ideas anti-lung drugs. The search focused studies published between January 1995 2024 explored use in cancer. A comprehensive literature was conducted SCOPUS, Google Scholar, PubMed, Web Science databases using keywords "luteolin" "lung cancer." By collecting previous literature, found has multiple mechanisms effects, including promotion apoptosis cells; inhibition cell proliferation, invasion metastasis; modulation immune responses. In addition, it can be used adjuvant radio-chemotherapy helps ameliorate complications. This summarizes structure, natural sources, physicochemical properties pharmacokinetics luteolin, focuses mechanism

Language: Английский

Citations

9
Artificial intelligence-driven identification and mechanistic exploration of synergistic anti-breast cancer compound combinations from Prunella vulgaris L.-Taraxacum mongolicum Hand.-Mazz. herb pair DOI Creative Commons
Chunlai Feng,

Jiaxi Cheng,

Mengqiu Sun

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

The Prunella vulgaris L. (PVL) and Taraxacum mongolicum Hand.-Mazz. (TH) herb pair, which is commonly used in traditional Chinese medicine (TCM), has been applied for the treatment of breast cancer. Although its efficacy validated, synergistic anti-breast cancer compound combinations within this pair their underlying mechanisms action remain unclear. This study aimed to identify validate PVL-TH using large-scale biomedical data, artificial intelligence experimental methods. first step was investigate effects various PVL TH extracts vitro cellular assays most effective superior extracts. These were subjected liquid chromatography-mass spectrometry (LC-MS) analysis constituent compounds. A deep learning-based prediction model, DeepMDS, predict multi-compound combinations. predicted experimentally validated at actual content ratios found Preliminary bioinformatics analyses conducted explore these We also compared from different geographical origins analyzed contents compounds assess representation anti-tumor effect corresponding TCM. results revealed that LC-MS identified 27 21 (50% ethanol extracts) TH, respectively. Based on compounds, DeepMDS model such as F973 (caffeic acid, rosmarinic p-coumaric esculetin), T271 (chlorogenic cichoric caffeic acid), T1685 scopoletin) single PVL, combinations, concentrations extracts, demonstrated activity could regulate tumor-related pathways synergistically, inhibiting tumor cell growth, inducing apoptosis, blocking cycle progression. Furthermore, concentration ratio total closely associated with origins. combination represent pair. provides insights into exploring representative complex

Language: Английский

Citations

1
Exploiting Nanotechnology for Drug Delivery: Advancing the Anti-Cancer Effects of Autophagy-Modulating Compounds in Traditional Chinese Medicine DOI Creative Commons
Zixian Liu, Tianming Lu,

Ruoning Qian

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2507 - 2528

Published: March 1, 2024

Background: Cancer continues to be a prominent issue in the field of medicine, as demonstrated by recent studies emphasizing significant role autophagy development cancer. Traditional Chinese Medicine (TCM) provides variety anti-tumor agents capable regulating autophagy. However, clinical application autophagy-modulating compounds derived from TCM is impeded their restricted water solubility and bioavailability. To overcome this challenge, utilization nanotechnology has been suggested potential solution. Nonetheless, current body literature on nanoparticles delivering TCM-derived for cancer treatment limited, lacking comprehensive summaries detailed descriptions. Methods: Up November 2023, research study was conducted gather relevant data using databases, including PubMed, ScienceDirect, Springer Link, Web Science, CNKI. The keywords utilized investigation included "autophagy", "nanoparticles", "traditional medicine" "anticancer". Results: This review analysis overcoming delivery challenges enhancing anti-cancer properties TCM. evaluation based synthesis different classes TCM, mechanisms action treatment, benefits reported various scholarly sources. findings indicate that shows availability thereby opening up plethora therapeutic avenues. Conclusion: Nanotechnology enhance efficacy traditional through regulation Keywords: autophagy, nano-delivery, anti-cancer, medicine

Language: Английский

Citations

7
Antioxidative and Anticancer Potential of Luteolin: A Comprehensive Approach Against Wide Range of Human Malignancies DOI Creative Commons

Mahwish,

Muhammad Imran, Hammad Naeem

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 1, 2025

ABSTRACT Luteolin is widely distributed phytochemical, a flavonoid, in kingdom plantae. with potential antioxidant activity prevent ROS‐induced damages and reduce oxidative stress which mainly responsible pathogenesis of many diseases. Several chemo preventive activities therapeutic benefits are associated luteolin. prevents cancer via modulation numerous pathways, that is, by inactivating proteins; such as procaspase‐9, CDC2 cyclin B or upregulation caspase‐9 caspase‐3, cytochrome C, A, CDK2, APAF‐1, turn inducing cell cycle arrest well apoptosis. It also enhances phosphorylation p53 expression level p53‐targeted downstream gene. By Increasing BAX protein expression; decreasing VEGF Bcl‐2 it can initiate stimulate mitochondrial‐modulated functions to cause cellular death. levels p‐Akt, p‐EGFR, p‐Erk1/2, p‐STAT3. plays positive role against cardiovascular disorders improving cardiac function, the release inflammatory cytokines enzymes, prevention fibrosis hypertrophy; CTGF, TGFβ1, ANP, Nox2, Nox4 gene expressions. Meanwhile suppresses TGFβ1 JNK. helps fight diabetes inhibition alpha‐glucosidase ChE activity. catalase, superoxide dismutase, GS4. improve blood glucose, insulin, HOMA‐IR, HbA1c levels. This review an attempt elaborate molecular targets luteolin its modulating irregularities pathways overcome severe outcomes during diseases including cancer, disorders, diabetes, obesity, inflammation, Alzheimer's disease, Parkinson's hepatic renal brain injury, asthma. As has enormous benefits, could be candidate future drug development strategies.

Language: Английский

Citations

0
Luteolin inhibits diffuse large B-cell lymphoma cell growth through the JAK2/STAT3 signaling pathway DOI Creative Commons
Xin-Zhuo Zhan, Bo Yang, Yu Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 20, 2025

Luteolin, a flavonoid present in botanical drugs, plants, and dietary sources, has demonstrated anticancer properties against various tumors, yet its role diffuse large B-cell lymphoma (DLBCL) remains unclear. This study aimed to uncover the molecular mechanism of luteolin DLBCL treatment using combination vitro vivo experiments computational analysis. Human cell lines U2932 OCI-LY10 were utilized assess luteolin's impact on growth, apoptosis, cycle progression, modulation JAK2/STAT3 pathway proteins. In vivo, tumor-bearing nude mice model was employed evaluate antitumor efficacy effects protein expression. Additionally, dynamics simulations conducted explore interaction between JAK2. The findings revealed that significantly suppressed proliferation, induced arrested at G2/M phase both lines. mouse model, effectively inhibited tumor growth downregulated expression phosphorylated JAK2 STAT3 without altering total levels STAT3. Computational analysis indicated stable binding Collectively, these results suggest anti-DLBCL activity may be mediated through regulation signaling pathway, positioning it as potential therapeutic agent for DLBCL.

Language: Английский

Citations

0
Phytoconstituents as emerging therapeutics for breast cancer: Mechanistic insights and clinical implications DOI Creative Commons

Mahalakshmi Devaraji,

Punniyakoti T. Veeraveedu

Cancer Pathogenesis and Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Luteolin Inhibits Dexamethasone‐Induced Osteoporosis by Autophagy Activation Through miR‐125b‐5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study DOI Creative Commons
Liang Tang, Xinyu Fan, Yongqing Xu

et al.

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(3)

Published: March 1, 2025

ABSTRACT Luteolin (LUT) has been suggested as an inhibitor of osteoporosis (OP). This investigation examines the pivotal role miR‐125b‐5p/SIRT3/AMPK/mTOR pathway in mediating luteolin‐induced effects on OP. Mesenchymal stem cells derived from bone marrow (BMSCs) were exposed to dexamethasone (DEX) create vitro model Following treatment with luteolin, levels miR‐125b‐5p and SIRT3 quantified using reverse transcription polymerase chain reaction. Moreover, SIRT3, AMPK, mTOR protein levels, osteogenesis (OPN, Runx2, OSX, OCN), autophagy (p62, ATG5, LC3, BECN1) evaluated ELISA. Additionally, specific mimics siRNA constructed overexpress or downregulate SIRT3. Furthermore, animal models DEX‐induced OP assess LUT at doses 50 100 mg/kg/day histology, stereology, biochemistry, expression miR‐125b‐5p, SIRT3/AMPK/mTOR axis, markers autophagy. The findings revealed that suppressed expression, overexpressed downregulated BMSCs compared DEX ( p ‐value < 0.01). Interestingly, restored for 0.001). overexpression downregulation inhibited therapeutic properties. In animals, improved histology 0.05) while overexpressing AMPK RUNX2, OPN, OCN improved, was enhanced LUT‐treated rats. current could promote improve via activation through pathway.

Language: Английский

Citations

0
The mechanism of Weiqi decoction treating gastric cancer: a work based on network pharmacology and experimental verification DOI Creative Commons
Xu Huang, Zhihong Pan, Lei Shen

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: April 21, 2025

Abstract Background Weiqi Decoction (WQD) is an empirical prescription traditionally used in China for the treatment of precancerous gastric cancer (GC) lesions. This study aimed to elucidate potential pharmacological mechanisms WQD GC therapy. Methods Active ingredients, corresponding targets, and GC-related genes were identified using public databases. A protein–protein interaction (PPI) network was constructed via STRING database, functional enrichment analyses conducted DAVID platform. Gene expression survival performed GEPIA database. Molecular docking with AutoDock Vina visualized PyMOL. The effects on cell viability, proliferation, migration, invasion evaluated through CCK-8, colony formation, Transwell assays. Results contained 43 active ingredients targeting 751 genes, including 458 targets. Quercetin, luteolin, kaempferol as key compounds. PPI analysis revealed nine core TP53 SRC , which may mediate anti-GC WQD. GO indicated involvement 726 biological processes, 91 cellular components, 177 molecular functions, while KEGG pathway suggested modulation AGE-RAGE, PI3K-Akt, HIF-1 signaling pathways. database confirmed that EP300 HSP90AA1 HSP90AB1 highly expressed GC. demonstrated strong binding affinities between compounds In vitro experiments further validated extract inhibited invasion. Conclusion exhibits therapeutic against by regulating multiple targets These findings provide mechanistic insights into actions treatment.

Language: Английский

Citations

0
Phytochemical‐mediated modulation of autophagy and endoplasmic reticulum stress as a cancer therapeutic approach DOI Creative Commons

Mazoun Al Azzani,

Zohra Nausheen Nizami, Rym Magramane

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: 38(9), P. 4353 - 4385

Published: July 3, 2024

Autophagy and endoplasmic reticulum (ER) stress are conserved processes that generally promote survival, but can induce cell death when physiological thresholds crossed. The pro-survival aspects of these exploited by cancer cells for tumor development progression. Therefore, anticancer drugs targeting autophagy or ER to and/or block the being investigated extensively. Consistently, several phytochemicals have been reported exert their effects modulating stress. Various (e.g., celastrol, curcumin, emodin, resveratrol, among others) activate unfolded protein response stress-mediated apoptosis through different pathways. Similarly, various mechanisms (namely mechanistic target Rapamycin [mTOR] inhibition). However, phytochemical-induced function either as a cytoprotective mechanism programmed type II. Interestingly, at times, same phytochemical 6-gingerol, shikonin, II depending on cellular contexts, such type. Although there is well-documented interplay between stress, only one-way modulation was noted with some (carnosol, capsaicin, cryptotanshinone, guangsangon E, kaempferol, δ-tocotrienol): stress-dependent autophagy. Plant extracts sources potent while numerous in preclinical clinical studies, search novel ongoing from plant used traditional medicine Origanum majorana). Nonetheless, translation phytochemicals, promising avenue therapeutics, hindered limitations need be addressed future studies.

Language: Английский

Citations

3