Regulation of Apoptosis, Autophagy, and Metastasis by Luteolin in Human Bladder Cancer EJ138 Cells: An Experimental Study

Deleted Journal, Journal Year: 2024, Volume and Issue: 23(1)

Published: Nov. 16, 2024

Chemotherapy remains a primary approach to cancer treatment, widely applied in bladder (BC). However, the various side effects and resistance associated with chemotherapeutic drugs pose significant challenges BC therapy, prompting interest natural compounds like luteolin. Studies focus on its key biological processes involved BC, including metastasis, apoptosis, autophagy. This study investigated regulation of mRNA expression genes apoptosis (BCL2, P53), autophagy (ULK1, ATG12), metastasis (MMP2, MMP9) malignant cells treated was an vitro experimental study. EJ138 were concentrations luteolin, impact cell viability, proliferation, gene assessed.The cytotoxic effect luteolin evaluated using MTT assay after 24- 48-hour treatments different concentrations. Flow cytometry performed examine luteolin's anti-proliferative effect, RT-PCR used analyze BCL2, P53, ULK1, ATG12, MMP2, MMP9 genes. results confirmed that reduced proliferation rate cells. indicated increased death following treatment. findings demonstrated upregulated ATG12 while downregulating BCL2 expression. treatment did not significantly alter MMP2 levels. These indicate exerts by dysregulating Therefore, shows potential as effective anti-cancer agent for therapy.

Language: Английский

Investigating the potential hub genes and mechanisms of Artemisia annua L. against breast cancer based on network pharmacology and molecular docking

South African Journal of Botany, Journal Year: 2023, Volume and Issue: 165, P. 163 - 175

Published: Dec. 29, 2023

Language: Английский

Luteolin Regulating Synthesis and Catabolism of Osteoarthritis Chondrocytes Via Activating Autophagy

Published: Jan. 1, 2023

Osteoarthritis (OA) is a common bone and joint disease characterized by degeneration. The imbalance between the synthesis breakdown of chondrocytes major contributing factor to OA. A potential treatment for OA could involve targeting degenerative changes in cartilage tissue degradation. Previous studies have shown close relationship autophagy regulation chondrocyte breakdown. Activation has been found alleviate degeneration tissue. Currently, primary focus osteoarthritis symptom relief, as there no effective medication halt progression. In previous studies, luteolin, flavonoid Chinese herbal medicine, activate reduce expression MMP1 ADAMTS-5. this study, an vitro model was created using stimulated IL-1β, different concentrations luteolin were used treatment. Treatment with significantly increased levels factors Aggrecan Collagen II, while decreasing decomposition MMP-13 Furthermore, when inhibited Chloroquine, imbalances anabolic catabolic activities induced IL-1β reversed. vivo knee medial meniscal instability (DMM), administered therapeutic schedule. After 12 weeks, tissues mice collected analysis. Immunofluorescence immunohistochemical staining showed decrease level P62 increase Beclin-1 tissues. Additionally, wear joints relieved safranin O fast green staining. findings our study highlight significant role effectively reversing IL-1β. Our results strongly indicate that be developed novel agent osteoarthritis, offering promising prospects future drug development.

Language: Английский