EFFECT OF MELATONIN ON PLASMA PYRUVATE LEVELS IN ALLOXAN-INDUCED DIABETIC RATS UNDER DIFFERENT PHOTOPERIODS DOI Open Access

О.Yu. Kushnir,

І.М. Yaremii,

О.Yu. Kushnir

et al.

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(4), P. 201 - 205

Published: Dec. 26, 2024

Some studies suggest that melatonin exerts a stimulating effect by reducing lactate dehydrogenase activity and shifting the balance of redox enzymes towards predominance Krebs cycle, thereby favoring aerobic processes are more efficient for cellular energy production. Therefore, an in-depth investigation this is warranted. The aim experimental study was to examine impact on levels pyruvic acid in plasma rats under physiological conditions alloxan-induced diabetes, with focus hypo- hyperfunction pineal gland. Methods. conducted 50 sexually mature male outbred white weighing 0.18–0.20 kg. Photoperiodic changes were simulated over one-week period, following conditions: 1) natural light/dark cycle from March 19 25, 2024, averaging 12:12 hours; 2) artificial (light 8:00 a.m. p.m., illumination at 500 lux); 3) constant light (500 lux) 24 4) darkness hours. Alloxan diabetes induced intraperitoneal injection 5% alloxan monohydrate solution dose 170 mg/kg. Fasting glucose measured using One Touch Ultra Easy device (Johnson & Johnson, USA). divided into four groups based lighting intact rats; control group, receiving daily injections (10 mg/kg) 8 one week (Sigma, USA); mellitus; mellitus, starting 5th day post-alloxan week. Animals euthanized decapitation ether anesthesia 12th experiment. Pyruvate blood standard methods. Statistical analysis performed Statistica 10 (StatSoft Inc.). Results. It known presence hyperglycemia intensifies free radical oxidation. According data obtained us, diabetic rats, high level accompanied increase pyruvate serum. This happens due dysregulation decrease insulin. In case, turns lactate, creating development lactic acidosis. Staying dark deprivation hours) led 15% serum compared animals. A similar situation observed group Under round-the-clock animals, indicators content increased even comparison animals equinox average 22% higher. However, exposure (24 hours darkness) 10% 13% when activation oxidation melatonin. fasting 24-hour negated smaller deviations equinox. has been established short daytime photoperiod (8 light: 16 characterized secretion. these conditions, antioxidant protection immune system improved addition, pinealectomized reduced glutathione peroxidase tissues absence introduction (under - normalization). regardless influence did not differ conditions. ability improve uptake through transporters investigated. there as activator dehydrogenase. realised possibility inhibit kinase activate allowing transformation acetyl-CoA sending mitochondria. Conclusion. both normal enhances efficiency pathways.

Language: Английский

EFFECT OF MELATONIN ON PLASMA PYRUVATE LEVELS IN ALLOXAN-INDUCED DIABETIC RATS UNDER DIFFERENT PHOTOPERIODS DOI Open Access

О.Yu. Kushnir,

І.М. Yaremii,

О.Yu. Kushnir

et al.

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(4), P. 201 - 205

Published: Dec. 26, 2024

Some studies suggest that melatonin exerts a stimulating effect by reducing lactate dehydrogenase activity and shifting the balance of redox enzymes towards predominance Krebs cycle, thereby favoring aerobic processes are more efficient for cellular energy production. Therefore, an in-depth investigation this is warranted. The aim experimental study was to examine impact on levels pyruvic acid in plasma rats under physiological conditions alloxan-induced diabetes, with focus hypo- hyperfunction pineal gland. Methods. conducted 50 sexually mature male outbred white weighing 0.18–0.20 kg. Photoperiodic changes were simulated over one-week period, following conditions: 1) natural light/dark cycle from March 19 25, 2024, averaging 12:12 hours; 2) artificial (light 8:00 a.m. p.m., illumination at 500 lux); 3) constant light (500 lux) 24 4) darkness hours. Alloxan diabetes induced intraperitoneal injection 5% alloxan monohydrate solution dose 170 mg/kg. Fasting glucose measured using One Touch Ultra Easy device (Johnson & Johnson, USA). divided into four groups based lighting intact rats; control group, receiving daily injections (10 mg/kg) 8 one week (Sigma, USA); mellitus; mellitus, starting 5th day post-alloxan week. Animals euthanized decapitation ether anesthesia 12th experiment. Pyruvate blood standard methods. Statistical analysis performed Statistica 10 (StatSoft Inc.). Results. It known presence hyperglycemia intensifies free radical oxidation. According data obtained us, diabetic rats, high level accompanied increase pyruvate serum. This happens due dysregulation decrease insulin. In case, turns lactate, creating development lactic acidosis. Staying dark deprivation hours) led 15% serum compared animals. A similar situation observed group Under round-the-clock animals, indicators content increased even comparison animals equinox average 22% higher. However, exposure (24 hours darkness) 10% 13% when activation oxidation melatonin. fasting 24-hour negated smaller deviations equinox. has been established short daytime photoperiod (8 light: 16 characterized secretion. these conditions, antioxidant protection immune system improved addition, pinealectomized reduced glutathione peroxidase tissues absence introduction (under - normalization). regardless influence did not differ conditions. ability improve uptake through transporters investigated. there as activator dehydrogenase. realised possibility inhibit kinase activate allowing transformation acetyl-CoA sending mitochondria. Conclusion. both normal enhances efficiency pathways.

Language: Английский

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