Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection DOI Creative Commons
Niharika Bala,

Ramish H. Rafay,

Sarah C. Glover

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 25 - 25

Published: Dec. 28, 2024

Background: The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for causing the Coronavirus disease 2019 (COVID-19) outbreak. While mutations cause emergence of new variants, ancestral SARS-CoV-2 strain unique among other strains. Methods: Various clinical parameters, activity cathepsin proteases, and concentration various proteins were measured in urine samples from COVID-19-negative participants COVID-19-positive participants. Urinary extracellular vesicles (uEVs) isolated two groups used proteomic analysis subsequent pathway analyses. Results: Activity levels S L greater C-reactive protein, transmembrane serine protease 2, klotho protein significantly There was a amount uEVs COVID-19 group found to be enriched group. Pathway analyses proteomics data showed most identified involved signal transduction, stress response, metabolism, transport. predominantly associated with cellular membranes function cytoskeleton, enzyme regulation, transduction. Conclusions: Taken together, our identify novel urinary biomarkers that could further investigate long-term effects infection.

Language: Английский

New insights into the role of Klotho in inflammation and fibrosis: molecular and cellular mechanisms DOI Creative Commons
Xinyue Zhao,

Donghe Han,

Chun Zhao

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 6, 2024

As the body's defense mechanism against damage and infection, inflammatory response is a pathological process that involves range of cells cytokines. A healthy helps body repair by eliminating dangerous irritants. However, tissue fibrosis can result from an overly intense or protracted response. The anti-aging gene Klotho suppresses oxidation, delays aging, fosters development various organs. Numerous investigations conducted in last few years have discovered expression changed variety clinical diseases strongly linked to course outcome disease. functions as co-receptor for FGF humoral factor mediates intracellular signaling pathways such transforming growth β (TGF-β), toll-like receptors (TLRs), nuclear factor-kappaB (NF-κB), renin -angiotensin system (RAS), mitogen-activated protein kinase (MAPK). It also interferes with phenotype function cells, monocytes, macrophages, T B cells. Additionally, it regulates production factors. This article aims examine Klotho's scientific advances terms order provide novel therapy concepts fibrotic disorders.

Language: Английский

Citations

8
Soluble average Klotho level as a prognostic marker for acute kidney injury outcomes: a 90-day follow-up study DOI
Shasha Li, Yan Liang,

Jian-hua Feng

et al.

Biomarkers in Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 8

Published: March 22, 2025

Background Klotho is highly expressed in the kidney and confers pleiotropic protective effects. This study aimed to assess soluble average level its relationship renal function outcomes participants with acute injury (AKI).

Language: Английский

Citations

0
Lessons from Klotho mouse models to understand mineral homeostasis DOI Creative Commons
Teodora V. Grigore, Malou Zuidscherwoude, Hannes Olauson

et al.

Acta Physiologica, Journal Year: 2024, Volume and Issue: 240(10)

Published: Aug. 23, 2024

Abstract Aim Klotho, a key component of the endocrine fibroblast growth factor receptor—fibroblast axis, is multi‐functional protein that impacts renal electrolyte handling. The physiological significance Klotho will be highlighted in regulation calcium, phosphate, and potassium metabolism. Methods In this review, we compare several murine models with different targeted deletions insights into molecular function these offer. Results vivo, deficiency associated severely impaired mineral metabolism, consequences on growth, longevity disease development. Additionally, explore perspectives pathology vascular events, as well potential treatment options. Conclusion This comprehensive review emphasizes use to shed light deciphering vivo mechanisms handling, novel therapeutic interventions.

Language: Английский

Citations

2
Klotho as an Early Marker of Acute Kidney Injury Following Cardiac Surgery: A Systematic Review DOI Creative Commons
Konstantinos S. Mylonas,

Panagiotis Karakitsos,

Alireza Tajik

et al.

Journal of Cardiovascular Development and Disease, Journal Year: 2024, Volume and Issue: 11(5), P. 135 - 135

Published: April 28, 2024

Acute kidney injury is a common complication following cardiac surgery (CSA-AKI). Serum creatinine levels require minimum of 24–48 h to indicate renal injury. Nevertheless, early diagnosis remains critical for improving patient outcomes. A PRISMA-compliant systematic review the PubMed and CENTRAL databases was performed assess role Klotho as predictive biomarker CSA-AKI (end-of-search date: 17 February 2024). An evidence quality assessment four included studies with Newcastle–Ottawa scale. Among 234 patients studied, 119 (50.8%) developed postoperatively. above 120 U/L immediately postoperatively correlated an area under curve (AUC) 0.806 90% sensitivity. Additionally, postoperative serum ratio 0.695 showed 94.7% sensitivity 87.5% specificity, AUC 92.4%, maintaining its prognostic validity up three days. Urinary immunoreactivity better maintained in samples obtained via direct catheterization rather than indwelling catheter collection bags. Storage at −80 °C necessary delayed testing. Optimal timing both urine measurements from end cardiopulmonary bypass time first ICU lab tests. In conclusion, could be promising CSA-AKI. Standardization measurement protocols larger are needed validate these findings.

Language: Английский

Citations

1
Soluble Klotho Level as a Prognostic Marker for Acute Kidney Injury Outcomes: A 90-Day Follow-Up Study DOI
Shasha Li, Lixia Yu, Jianhua Feng

et al.

Published: Jan. 1, 2024

Background and aims: Klotho is highly expressed in the kidney confers pleiotropic protective effects, yet its clinical significance progression of acute injury (AKI) still not illustrated. Thus, we assessed soluble (sKlotho) level relationship to renal function outcomes participants with AKI. Materials methods: We recruited 102 AKI 30 healthy controls (HCs). For patients AKI, serum sKlotho levels upon admission discharge from hospital were measured assess between function. Individuals retrospectively followed up for 90 days determine association their average short-term outcomes. Results: The baseline individuals at was significantly lower than that HCs, even after age hemoglobin matching. stage 3 1 or 2. restored along improvement discharge. During follow-up, a average, but baseline, predicted more adverse (no recovery death). Conclusion: decreased represented severity. inversely correlated detrimental may have potential diagnostic predictive roles

Language: Английский

Citations

0
Opportunistic infections changed before and after SARS-CoV-2 infection in inflammatory bowel disease patients: a retrospective single-center study in China DOI Creative Commons
Zhenzhen Fan, Zhou He, Jiaqi Zhang

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 24, 2024

SARS-CoV-2 transmission has become a serious worldwide public health concern. However, there is currently insufficient data to determine whether infection would affect opportunistic infections in inflammatory bowel disease (IBD) patients. A retrospective study included 451 IBD patients (294 UC and 157 CD). The were divided into two groups: before after infection, outcomes measured for these groups. primary outcome was the presence distribution of infections. secondary factors associated with infections, based on which nomogram prediction model developed validated. After proportion by Clostridium difficile (21.31% vs. 14.01%, p = 0.044) Epstein-Barr virus (13.93% 4.35%, 0.001) significantly higher compared that before. Conversely, hepatitis B (3.69% 10.14%, 0.006) herpes simplex type I (1.23% 0.04) lower infection. Additionally, pre-SARS-CoV-2 include duration illness, red blood cell count, comorbid chronic illnesses, alcohol consumption, while post-SARS-CoV-2 risk involve corticosteroid use, protein level, high-sensitivity C-reactive protein. been shift occurrence among It might be attributed use corticosteroids also strengthening containment measures, heightened awareness, widespread vaccination.

Language: Английский

Citations

0
Klotho Deficiency in Severe COVID-19: A Unifying Hypothesis DOI Creative Commons
Natalia Campos‐Obando, M. Carola Zillikens, Román F. Macaya

et al.

COVID, Journal Year: 2024, Volume and Issue: 4(12), P. 1833 - 1850

Published: Nov. 22, 2024

COVID-19 is characterized by a striking similarity to premature aging. Its clinical manifestations range from asymptomatic critical illness. No single central agent has been demonstrated so far. We present Klotho, an antiaging protein, as key factor in pathophysiology. There epidemiological evidence that both acute and chronic uses of Klotho agonists have beneficial effect reducing severity mortality. A review the PubMed epidemiological, clinical, mechanistic supports role for deficit determinant severe COVID-19. Clinical data support idea use protects against its may be beneficial. propose unifying hypothesis low levels play COVID-19, while increasing can through prevention kidney injury (AKI) potential antiviral effects. Further research needed.

Language: Английский

Citations

0
Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection DOI Creative Commons
Niharika Bala,

Ramish H. Rafay,

Sarah C. Glover

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 25 - 25

Published: Dec. 28, 2024

Background: The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for causing the Coronavirus disease 2019 (COVID-19) outbreak. While mutations cause emergence of new variants, ancestral SARS-CoV-2 strain unique among other strains. Methods: Various clinical parameters, activity cathepsin proteases, and concentration various proteins were measured in urine samples from COVID-19-negative participants COVID-19-positive participants. Urinary extracellular vesicles (uEVs) isolated two groups used proteomic analysis subsequent pathway analyses. Results: Activity levels S L greater C-reactive protein, transmembrane serine protease 2, klotho protein significantly There was a amount uEVs COVID-19 group found to be enriched group. Pathway analyses proteomics data showed most identified involved signal transduction, stress response, metabolism, transport. predominantly associated with cellular membranes function cytoskeleton, enzyme regulation, transduction. Conclusions: Taken together, our identify novel urinary biomarkers that could further investigate long-term effects infection.

Language: Английский

Citations

0