Advanced UPLC-MS/MS Method for the Quantification of SIPI6398 in Rat Plasma and Its Pharmacokinetic Characterization DOI Creative Commons
Chen Fan,

Shunjun Ma,

Runrun Wang

et al.

Journal of Analytical Methods in Chemistry, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 7

Published: May 6, 2024

SIPI6398 is a novel anti-schizophrenia agent with new mechanism of action and demonstrates better target selectivity safety compared to its competitors. However, few in vivo studies on the pharmacokinetics bioavailability have been performed. A rapid simple ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach was developed for accurate quantification rat plasma. protein precipitation acetonitrile-methanol (9 : 1, v/v) used treat Chromatography performed UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 μm) at flow rate 0.4 ml/min. The mobile phase consisted acetonitrile-water (with 0.1% formic acid) gradient elution used, time 4 minutes. Quantitative analysis using electrospray ionization (ESI) positive ion detection mode multiple reaction monitoring (MRM) mode. To evaluate bioavailability, administered rats two different ways: oral (4 mg/kg) intravenous (2 administration. calibration curve UPLC-MS/MS shows excellent linearity range 1-2000 ng/mL an r value above 0.99. precision, accuracy, recovery, matrix effect, stability results all meet criteria established biological analytical methods. method successfully applied it study SIPI6398. calculated be 13.2%. These potential contribute towards more comprehensive comprehension

Language: Английский

Zhichuanling injection improves bronchial asthma by attenuation airway inflammation and epithelia-mesenchymal transition DOI

Kerui Ren,

Ben Niu, Hongge Liang

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: 344, P. 119540 - 119540

Published: Feb. 26, 2025

Language: Английский

Citations

0
Unlocking the nutritional profile of apricot (Prunus armeniaca L) kernel as a valuable by-product for future exploration DOI Creative Commons
Pravin Ojha, Xia Tang,

Zhou Liangfu

et al.

Future Foods, Journal Year: 2025, Volume and Issue: unknown, P. 100632 - 100632

Published: April 1, 2025

Language: Английский

Citations

0
Prediction of the Future Evolution Trends of Prunus sibirica in China Based on the Key Climate Factors Using MaxEnt Modeling DOI Creative Commons
Jiazhi Wang, Jiming Cheng, Chao Zhang

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 973 - 973

Published: Nov. 25, 2024

Mountain apricot (Prunus sibirica) is an important fruit tree variety, and has a wide range of planting application value in China even the world. However, current research on suitable distribution area P. sibirica still inconclusive. In this study, we retrieved data for from Global Biodiversity Information Facility (GBIF), identified six key environmental factors influencing its through cluster analysis. Using these selected climate points China, applied maximum entropy model (MaxEnt) to evaluate 1160 candidate models parameter optimization. The final results predict potential under as well two future scenarios (SSPs126 SSPs585). This study shows that optimized with (AUC = 0.897, TSS 0.658) outperforms full using nineteen 0.894, 0.592). Under high-emission scenario (SSPs585), highly habitat expected gradually shrink towards southeast northwest, while expanding northeast southwest. After 2050s, habitats are projected completely disappear Shandong, new areas may emerge Tibet. Additionally, total increase future, more significant expansion (SSPs585) compared low-emission (SSPs126) (7.33% vs. 0.16%). Seasonal changes precipitation most influential factor driving sibirica.

Language: Английский

Citations

3
Pharmacokinetics of IMM‐H012 in rats using ultra‐performance liquid chromatography‐tandem mass spectrometry DOI

Shujuan Wu,

Jialei Wu,

Longquan Lin

et al.

Biomedical Chromatography, Journal Year: 2024, Volume and Issue: 38(8)

Published: May 28, 2024

Abstract The present study examined the pharmacokinetics of IMM‐H012 in rat plasma, utilizing ultra‐performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS). Internal standard cilostazol was employed, and plasma samples were processed using acetonitrile precipitation. A mobile phase (acetonitrile–0.1% formic acid water) with gradient elution used to achieve chromatographic separation a UPLC BEH C18 column. In multiple reaction monitoring mode, electrospray ionization MS/MS utilized positive mode. Based on findings, lower limit quantification 2 ng/mL, linearity found be acceptable within range 2–2000 ng/mL ( R > 0.995). intra‐day inter‐day precision relative deviation less than 14% plasma. matrix effect 102%–107%, accuracy ranged from 92% 113%. Pharmacokinetics rats after oral administration successfully studied UPLC‐MS/MS.

Language: Английский

Citations

1
Genetic and therapeutic for oral lichen planus and diabetes mellitus: a comprehensive study DOI Creative Commons

Manman Yao,

Ying-Chun Lu,

Tiejun Liu

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 15, 2024

This study employed a bidirectional Mendelian Randomization (MR) approach to explore the causal relationships between Oral Lichen Planus (OLP), diabetes mellitus (DM), and glycemic control. It also aims identify potential pharmacological herbal treatments that effectively address both OLP metabolic dysfunctions associated with DM.

Language: Английский

Citations

1
Botanical drugs and their natural compounds: a neglected treasury for inhibiting the carcinogenesis of pancreatic ductal adenocarcinoma DOI Creative Commons
Yunfei Dai,

Xi Guan,

Fangyue Guo

et al.

Pharmaceutical Biology, Journal Year: 2024, Volume and Issue: 62(1), P. 853 - 873

Published: Nov. 9, 2024

Pancreatic ductal adenocarcinoma (PDAC), which is characterized by its malignant nature, presents challenges for early detection and associated with a poor prognosis. Any strategy that can interfere the beginning or earlier stage of PDAC greatly delays disease progression. In response to this intractable problem, exploration new drugs critical reduce incidence PDAC.

Language: Английский

Citations

1
Mandelonitrile lyase MDL2-mediated regulation of seed amygdalin and oil accumulation of Prunus Sibirica DOI Creative Commons
Feng Chen,

Junxin Zang,

Zirui Wang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 21, 2024

The Prunus sibirica seeds with rich oils has great utilization, but contain amygdalin that can be hydrolyzed to release toxic HCN. Thus, how effectively reduce seed content of P. is an interesting question. Mandelonitrile known as one key intermediate metabolism, which mandelonitrile lyase (MDL) family member essential for its dissociation destined low accumulation in still remains enigmatic. An integration our recent 454 RNA-seq data, and detection, qRT-PCR analysis function determination described a critical attempt determine MDL highlight governing catabolism better developing edible oil biodiesel China.

Language: Английский

Citations

0
Advanced UPLC-MS/MS Method for the Quantification of SIPI6398 in Rat Plasma and Its Pharmacokinetic Characterization DOI Creative Commons
Chen Fan,

Shunjun Ma,

Runrun Wang

et al.

Journal of Analytical Methods in Chemistry, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 7

Published: May 6, 2024

SIPI6398 is a novel anti-schizophrenia agent with new mechanism of action and demonstrates better target selectivity safety compared to its competitors. However, few in vivo studies on the pharmacokinetics bioavailability have been performed. A rapid simple ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach was developed for accurate quantification rat plasma. protein precipitation acetonitrile-methanol (9 : 1, v/v) used treat Chromatography performed UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 μm) at flow rate 0.4 ml/min. The mobile phase consisted acetonitrile-water (with 0.1% formic acid) gradient elution used, time 4 minutes. Quantitative analysis using electrospray ionization (ESI) positive ion detection mode multiple reaction monitoring (MRM) mode. To evaluate bioavailability, administered rats two different ways: oral (4 mg/kg) intravenous (2 administration. calibration curve UPLC-MS/MS shows excellent linearity range 1-2000 ng/mL an r value above 0.99. precision, accuracy, recovery, matrix effect, stability results all meet criteria established biological analytical methods. method successfully applied it study SIPI6398. calculated be 13.2%. These potential contribute towards more comprehensive comprehension

Language: Английский

Citations

0