Cited by Pharmacological evaluation and binding behavior of 4,4′-diamino-2,2′-stilbene disulfonic acid with metal ions using spectroscopic techniques

Isolation, invitro, invivo anti-inflammatory, analgesic and antioxidant potential of Habenaria plantegania Lindl DOI

Osama M. Alshehri,

Madeeha Shabnam,

Saeed Ahmed Asiri

et al.

Inflammopharmacology, Journal Year: 2024, Volume and Issue: 32(2), P. 1353 - 1369

Published: Feb. 9, 2024

Language: Английский

Citations

Modification of 4-(4-chlorothiophen-2-yl)thiazol-2-amine derivatives for the treatment of analgesia and inflammation: synthesis and in vitro, in vivo, and in silico studies DOI

Mater H. Mahnashi, Umer Rashid, Hassan H. Almasoudi

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 29, 2024

Inflammation is a protective response to variety of infectious agents. To develop new anti-inflammatory drug, we explored pharmacologically important thiazole scaffold in this study. In multi-step synthetic approach, synthesized seven derivatives ( 5a – 5g ). Initially, examined the vitro potentials our compounds using COX-1, COX-2, and 5-LOX enzyme assays. After confirmation, potential were subjected vivo analgesic studies. The hot plate method was used for analgesia, carrageenan-induced inflammation also assayed. Overall, all proved be potent inhibitors COX-2 compared celecoxib (IC 50 0.05 μM), exhibiting IC values range 0.76–9.01 μM .Compounds 5b , 5d 5e dominant selective with lowest selectivity index (SI) 42, 112, 124, respectively. Similarly, COX-1 assay, relatively less but still encouraging. Standard aspirin exhibited an value 15.32 μM. results, once again, 23.08 38.46 μM, zileuton 11.00 Based on COX/LOX SI potencies, Compounds at concentrations 5, 10, 20 mg/kg body weight significant animal models. Furthermore, role various phlogistic both significantly anti-nociceptive assay. molecular docking interactions these two target proteins COX LOX further strengthened their use pathway inhibitions.

Language: Английский

Citations

Exploring pyrazolines as potential inhibitors of NSP3-macrodomain of SARS-CoV-2: synthesis and in silico analysis DOI

R. R. Joshi,

Harsh Gaikwad,

Bhavana Soge

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 4, 2025

COVID-19 has proved to be a global health crisis during the pandemic, and emerging JN.1 variant is potential threat. Therefore, finding alternative antivirals of utmost priority. In current report, we present synthesis new anti-viral pyrazoline compounds. Here report chemical scheme where β-aryl β-anilino ketones react with phenyl hydrazine in potassium hydroxide give corresponding 3,5-diarylpyrazoline. The protocol applicable variety β-amino tolerates several functional groups. This method efficient proceeds regioselectivity since β-Anilino group acts as protecting for alkenes chalcones. We identified NSP3-microdomain (Mac-1) SARS-CoV-2 putative target newly synthesized triaryl-2-pyrazoline molecular dynamics simulation-based free energy estimation suggests compounds 7a, 7d, 7 g, 7i, 7k, L promising Mac-1 inhibitors. detailed structural inspection MD simulation trajectories sheds light on involved Mac-1. data presented here expected guide design development better anti-SARS-CoV-2 therapies.

Language: Английский

Citations

Rating of In Vitro Cytotoxicity Activities and Anti-Xanthine Oxidase Activities of Some Non-Proteoneogenic Amino Acid Derivatives by Molecular Docking and Molecular Dynamics Studies DOI

Zuhal Alım, Serap Yalçın Azarkan, Namık Kılınç

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142497 - 142497

Published: April 1, 2025

Language: Английский

Citations

Evaluation of Habenaria aitchisonii Reichb. for antioxidant, anti-inflammatory, and antinociceptive effects with in vivo and in silico approaches DOI

Saeed Asiri,

Madeeha Shabnam,

Rehman Zafar

et al.

Frontiers in Chemistry, Journal Year: 2024, Volume and Issue: 12

Published: March 19, 2024

Habenaria aitchisonii Reichb was analyzed in this research, including its chemical composition and vitro antioxidant, anti-inflammatory, acute oral toxicity, antinociceptive activity. The chloroform ethyl acetate fractions were found to be the most powerful based on analgesic assays. toxicity of crude methanolic extract determined before vivo studies. acetic acid formalin tests used measure effect, potential mechanisms involved antinociception explored. carrageenan-induced paw edema test examine immediate anti-inflammatory many phlogistic agents determine specific mechanism. Furthermore, for ex activities, mice sacrificed, forebrain isolated, antioxidant levels glutathione (GSH), superoxide dismutase (SOD), thiobarbituric reactive substances (TBARS) catalase (CAT) estimated using a UV spectrophotometer. No seen at dosages up 3,000 mg/kg. impact much higher than standard drug. Both inflammatory neurogenic phases experiment revealed an effect fractions. In carrageenan assays, fraction (Ha.Chf) potent fraction. We further studied GC-MS plant total 18 compounds. mechanism, it observed that Ha.Chf inhibits COX-2 as well 5-LOX pathways. results exhibited species is good source phytocomponents like germacrone, which can employed sustainable natural therapeutic agent, supporting traditional use folk medicine conditions pain.

Language: Английский

Citations

Phenolic phytochemistry, in vitro, in silico, in vivo, and mechanistic anti-inflammatory and antioxidant evaluations of Habenaria digitata DOI

Hassan H. Almasoudi, Muhammad Saeed Jan, Mohammed H. Nahari

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 29, 2024

Excessive and imbalance of free radicals within the body lead to inflammation. The objective current research work was explore anti-inflammatory antioxidant potential isolated compounds from Habenaria digitata. In this study, phenolic were investigated for in vitro vivo along with enzyme. assayed via various enzymes like COX-1/2, 5-LOX ABTS, DPPH, H 2 O radical enzyme inhibitory assay. These also explored their activity examining SOD, CAT, GSH-Px, MDA levels brain, heart, liver. evaluated using carrageenan-induced pleurisy model mice. On basis initial screening compounds, most potent compound further mechanism. Furthermore, molecular docking study performed compound. identified by GC-MS/NMR analysis comparing its spectra library spectra. H. digitata 5-methylpyrimidine-24,4-diol (1), 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one (2), 2-isopropyl-5-methylphenol (3), 3-methoxy-4-vinylphenol ( 4 ), 2,6-dimethoxy-4-vinylphenol (5). assay, 1 having IC 50 values 0.98, 0.90, 5 μg/mL against , respectively. Similarly, COX1/2 ,compound again 42.76, 10.70, 7.40 μg/mL. Based on results, potential. Findings suggest that contains active effects. could be screened as drug candidates pharmaceutical research, targeting conditions associated oxidative stress inflammatory medicinal chemistry support ethnomedicinal use inflammation stress.

Language: Английский

Citations

Pharmacological evaluation and binding behavior of 4,4′-diamino-2,2′-stilbene disulfonic acid with metal ions using spectroscopic techniques DOI

Madeeha Shabnam,

Eman A. Alabdullkarem,

Muhammad Saeed Jan

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34639 - e34639

Published: July 1, 2024

Industrial and human activities contribute significantly to the environmental contamination of heavy metal ions (HMIs), which have detrimental effects on aquatic life, plants, animals, causing major toxicological problems. The commercially available

Language: Английский

Citations