Discovery of Active Ingredients Oleanolic Acid, Ursolic Acid, and Arbutin for in Vitro Anti-Melanoma: Network Pharmacology and Experimental Validation

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 1, 2025

Objective Antitumor effects of the medicinal herb Prinsepia utilis Royle ( P. utilis) have been reported. This study aimed to identify potential functional components and molecular mechanisms in treatment melanoma through network pharmacology experimental verification. Methods Bioinformatics database analysis platform Chinese medicine system were used obtain active utilis, targets predicted by Swiss Target Prediction database. Disease associated with retrieved from Genecard Network topology enrichment conducted screen out core related signal pathways. The docking method was employed evaluate target binding bioactive ingredients a biology approach performed predict verify mechanism treating melanoma. Results KEGG pathway revealed multiple cancer-related pathways signaling Oleanolic acid (OA), Ursolic (UA), Arbutin are utilis. crude extract able inhibit melanin A375 cells. compounds OA UA can significantly growth migration ability human cells while inducing apoptosis inhibiting tyrosinase activity synthesis. In contrast, arbutin does not show obvious performance these biological activities. Conclusion These findings suggest that promising as more effective anti-melanoma conclusion, studies protect against regulating tyrosine melanogenesis.

Language: Английский

Copper in melanoma: at the crossroad of protumorigenic and anticancer roles

Redox Biology, Journal Year: 2025, Volume and Issue: 81, P. 103552 - 103552

Published: Feb. 15, 2025

Language: Английский