Leveraging Single-Cell Multi-Omics to Decode Tumor Microenvironment Diversity and Therapeutic Resistance

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(1), P. 75 - 75

Published: Jan. 10, 2025

Recent developments in single-cell multi-omics technologies have provided the ability to identify diverse cell types and decipher key components of tumor microenvironment (TME), leading important advancements toward a much deeper understanding how heterogeneity contributes cancer progression therapeutic resistance. These are able integrate data from molecular genomic, transcriptomic, proteomics, metabolomics studies cells at resolution scale that give rise full cellular complexity TME. Understanding complex sometimes reciprocal relationships among cells, CAFs, immune ECs has led novel insights into their immense functions, which can consequences on behavior. In-depth uncovered evasion mechanisms, including exhaustion T metabolic reprogramming response hypoxia cells. Single-cell also revealed resistance such as stromal cell-secreted factors physical barriers extracellular matrix. Future examining specific pathways targeting approaches reduce TME will likely lead better outcomes with immunotherapies, drug delivery, etc., for treatments. incorporate data, spatial micro-environments, translation personalized therapies. This review emphasizes provide TME, revealing reprogramming, influences. aim guide development targeted therapies, highlighting role diversity shaping behavior treatment outcomes.

Language: Английский

Modelling Cancer Pathophysiology: Mechanisms and Changes in the Extracellular Matrix During Cancer Initiation and Early Tumour Growth

Cancers, Journal Year: 2025, Volume and Issue: 17(10), P. 1675 - 1675

Published: May 15, 2025

Cancer initiation and early tumour growth are complex processes influenced by multiple cellular microenvironmental factors. A critical aspect of progression is the dynamic interplay between cancer cells extracellular matrix (ECM), which undergoes significant alterations to support malignancy. The loss cell polarity an hallmark progression, disrupting normal tissue architecture fostering cancerous transformation. Circumstantially, cancer-associated microRNAs (miRNAs) regulate key oncogenic processes, including ECM remodelling, epithelial-to-mesenchymal transition (EMT), tumorigenic vascular development, further driving growth. alterations, particularly changes in stiffness mechanotransduction signals, create a supportive niche for cells, enhancing their survival, proliferation, invasion. EMT its subtype, epithelial-to-endothelial (EET), contribute plasticity, promote generation stem (CSCs), vascularisation. Furthermore, development like vasculogenesis angiogenesis sustaining growth, supplying oxygen nutrients hypoxic malignant within evolving microenvironments. This review explores mechanisms underlying these microenvironments, with emphasis on collective role It will delve into present vitro modelling strategies developed closely mimic pathophysiology. Understanding crucial developing targeted therapies aimed at cancer-promoting pathways improving clinical outcomes.

Language: Английский

Ultrastructural analysis of vasculogenic mimicry in rectal adenocarcinoma after neoadjuvant radiation therapy

Cardiometry, Journal Year: 2024, Volume and Issue: 33, P. 31 - 32

Published: Nov. 25, 2024

One of the hallmarks cancer is tumor angiogenesis, so development and testing angiogenesis inhibitors focus fundamental clinical research. However, some mechanisms evasion from anti-angiogenic therapy have been identified. They include activation compensatory/alternative pathways, vasculogenic mimicry, vessel co-option.

Language: Английский