Effects of Hispidulin on the Osteo/Odontogenic and Endothelial Differentiation of Dental Pulp Stem Cells DOI Creative Commons
Yeon Kim, Hyun-Joo Park, Mi-Kyoung Kim

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1740 - 1740

Published: Dec. 23, 2024

Background: Human dental pulp stem cells (HDPSCs) with multi-lineage differentiation potential and migration ability are required for HDPSC-based bone regeneration. Hispidulin is a naturally occurring flavonoid diverse pharmacological activities, but its effects on biological properties of HDPSCs remain unknown. Therefore, we investigated the hispidulin elucidated their underlying mechanisms. Methods: The osteo/odontogenic capacity was assessed using alkaline phosphatase (ALP) Alizarin Red S (ARS) staining. evaluated scratch wound assay. Furthermore, endothelial examined by capillary sprouting assay assessing CD31 expression. Results: significantly enhanced increased expression markers. HDPSCs, which mediated upregulation C-X-C chemokine receptor type 4 (CXCR4). treatment into cells, as evidenced marker In addition, demonstrated that activated ERK1/2 signaling, inhibition U0126 suppressed hispidulin-induced HDPSCs. Conclusions: These findings demonstrate effectively promotes differentiation, results suggest may have therapeutic applications in regeneration tissue engineering.

Language: Английский

Platelet-rich plasma ameliorates cartilage degradation in rat models of osteoarthritis via the OPG/RANKL/RANK system DOI Creative Commons

Qun Wu,

Xianbao Yao,

Nan Shan

et al.

Folia Histochemica et Cytobiologica, Journal Year: 2024, Volume and Issue: 62(3), P. 154 - 164

Published: Aug. 19, 2024

Introduction. Osteoarthritis (OA) is one of the most common degenerative joint diseases in elderly, which featured by degradation articular cartilage. Recently, platelet-rich plasma (PRP) injection into affected has become biological therapy for OA treatment. The OPG/RANKL/RANK signalling been reported to mediate progression. Our study aimed confirm whether PRP retards development through regulation system. Material and methods. rat models were induced medial menisci resection combined with anterior cruciate ligament transection. Four weeks after surgery, OA-induced rats received intra-articular 50 μ L once a week 6 weeks. Rats euthanised th injection. Rat knee joints subjected histopathological examination haematoxylin-eosin (H&E) safranin O staining. Osteoprotegerin (OPG), receptor activator nuclear factor kappa B (RANK), RANK ligand (RANKL) cartilage tested immunofluorescence staining western blotting. Serum interleukin-1β (IL-1β) interleukin-6 (IL-6) levels measured enzyme-linked immunosorbent assay (ELISA). Matrix metalloproteinase-13 (MMP-13), aggrecan, collagen Ⅱ, IL-1β, IL-6, tumour necrosis factor-alpha (TNF-α), kappa-B (NF-κB) mRNA protein expression examined real-time quantitative polymerase chain reaction (RT-qPCR) blotting, respectively. Results. Intra-articular injections significantly improved structural integrity enhanced synthesis glycosaminoglycans. reduced MMP-13 level but increased aggrecan Ⅱ rats. increase serum TNF-α concentrations as well MMP-13, decreased II reversed administration PRP. TNF-α, NF-κB whereas ameliorated these changes. Moreover, tissue OPG was further elevated did not change. RANKL offset administration. OPG/RANKL ratio, RANKL/RANK ratio Conclusions. mitigates inflammation experimental regulating

Language: Английский

Citations

1
Luteolin alleviates airway remodeling in asthma by inhibiting the epithelial-mesenchymal transition via β-catenin regulation DOI
Jingyu Quan, Dan Xie, Zihong Li

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156090 - 156090

Published: Oct. 3, 2024

Language: Английский

Citations

1
Yiqi Yangxue formula inhibits cartilage degeneration in knee osteoarthritis by regulating LncRNA-UFC1/miR-34a/MMP-13 axis DOI Creative Commons
Ting Zhao,

X. Y. Wang,

Zhaofu Li

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 337, P. 118930 - 118930

Published: Oct. 10, 2024

Language: Английский

Citations

0
Effects of Hispidulin on the Osteo/Odontogenic and Endothelial Differentiation of Dental Pulp Stem Cells DOI Creative Commons
Yeon Kim, Hyun-Joo Park, Mi-Kyoung Kim

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1740 - 1740

Published: Dec. 23, 2024

Background: Human dental pulp stem cells (HDPSCs) with multi-lineage differentiation potential and migration ability are required for HDPSC-based bone regeneration. Hispidulin is a naturally occurring flavonoid diverse pharmacological activities, but its effects on biological properties of HDPSCs remain unknown. Therefore, we investigated the hispidulin elucidated their underlying mechanisms. Methods: The osteo/odontogenic capacity was assessed using alkaline phosphatase (ALP) Alizarin Red S (ARS) staining. evaluated scratch wound assay. Furthermore, endothelial examined by capillary sprouting assay assessing CD31 expression. Results: significantly enhanced increased expression markers. HDPSCs, which mediated upregulation C-X-C chemokine receptor type 4 (CXCR4). treatment into cells, as evidenced marker In addition, demonstrated that activated ERK1/2 signaling, inhibition U0126 suppressed hispidulin-induced HDPSCs. Conclusions: These findings demonstrate effectively promotes differentiation, results suggest may have therapeutic applications in regeneration tissue engineering.

Language: Английский

Citations

0