Carthamus tinctorius L. protects cerebral ischemia/reperfusion injury via arachidonic acid/p53-mediated apoptosis axis DOI Creative Commons
Junren Chen,

Liujun Wu,

Xiaofang Xie

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Introduction Stroke is a debilitating disease and the second leading cause of death worldwide, which ischemic stroke dominant type. Carthamus tinctorius L., also known as safflower, has been used to treat cerebrovascular diseases, especially in many Asian countries. However, underlying mechanisms safflower preventing remains elusive. This study aims elucidate potential drug candidate for prevention delineate its protective effects rat model cerebral ischemia-reperfusion injury (CI/RI). Methods The aqueous extract (AESF) was verified using HPLC-UV, HPLC-MS, TLC. inhibitory effect AESF on platelet aggregation detected vitro zebrafish mice. A CI/RI rats established by middle artery occlusion reperfusion stroke. 2,3,5-triphenyltetrazolium chloride, hematoxylin eosin, Nissl’s staining were employed evaluate pathological changes brain tissue. In addition, metabolomics, ELISA, Western blot uncover molecular alteration induced AESF. Results significantly inhibited , reduced thrombogenesis zebrafish, prolonged clotting time alleviated neurological dysfunction, oedema, infarct size, histopathological damage ischemia-reperfusion, improved neuronal survival, increased serum levels SOD CAT, decreased iNOS NO. Metabolomics revealed that attenuated metabolic disturbances caused I/R via regulating 38 metabolites particularly related arachidonic acid (AA) metabolism. Moreover, elevated 6-keto-PGF 1α pivotal metabolite AA, downregulated protein expression p53, Bax, cleaved caspase-9, caspase-3, caspase-8, upregulated Bcl-2. Conclusion mitigated through aggregation, alleviating oxidative stress, suppressing apoptosis partially modulating AA metabolism/p53-mediated axis.

Language: Английский

Effects of Salmonella Typhimurium infection on intestinal flora and intestinal tissue arachidonic acid metabolism in Wenchang chickens DOI Creative Commons

Shenghong Chen,

Yaochen Xie,

Dingqian Guo

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 24, 2025

Salmonella infections can lead to intestinal inflammation and metabolic disorders in birds. However, whether arachidonic acid (ARA) metabolism is involved -induced remains unclear. This experiment investigated the changes cecal flora ARA Hainan Wenchang chickens infected with S. Typhimurium using 16s rDNA sequencing targeted metabolomics. The results showed that levels of metabolites were increased cecum tissue after infection , including prostaglandin E2 (PGE 2 ), F2α (PGF 2α lipoxin A4 (LXA4), ± 8(9)-EET, 11(12)-EET, 8,9-DiHETrE. content key enzymes for production (Phospholipase A2 PLA2 Cyclooxygenase-2 COX-2) chicken tissues was infection. relative mRNA inflammatory factors also infection, Interferon-γ (IFN-γ), Transforming growth factor-β1 (TGF-β1), Interleukin-4 (IL-4), Interleukin-6 (IL-6). In HD11 cells, use a cyclooxygenase (COX) inhibitor reduced COX-2 PGF induced by effectively response. addition, number beneficial genera (e.g., Bifidobacterium Lactobacillus Odorobacterium ) significantly . present study revealed structure -infected chickens. this demonstrated activates pathway, which turn mediates development provide data support theoretical prevention control avian salmonellosis.

Language: Английский

Citations

1
Bile acid metabolism in health and ageing-related diseases DOI
Xiaojun Li,

Chu Fang,

Ruihua Zhao

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 225, P. 116313 - 116313

Published: May 22, 2024

Language: Английский

Citations

7
Shenzhuo formulation ameliorates diabetic nephropathy by regulating cytochrome P450-mediated arachidonic acid metabolism DOI
Zhongyong Zhang, Yuming Wang, Ning Wang

et al.

World Journal of Diabetes, Journal Year: 2025, Volume and Issue: 16(5)

Published: April 24, 2025

BACKGROUND Diabetic nephropathy (DN) is a major complication of diabetes, marked by progressive renal damage and an inflammatory response. Although research has investigated the pathological mechanisms underlying DN, effective treatment options remain limited. AIM To evaluate therapeutic impact Shenzhuo formulation (SZF) on DN mouse model to examine its potential molecular using transcriptomic metabolomic approaches. METHODS We established through high-fat diet combined with streptozotocin (STZ) injection, followed SZF treatment. analyzed SZF’s effects gene expression metabolite profiles in tissues mice transcriptomics metabolomics techniques. Additionally, based non-targeted findings, we further assessed influence factors related cytochrome P450 (CYP450)-mediated arachidonic acid (AA) metabolism pathway, as well inflammation oxidative stress. RESULTS intervention significantly decreased hyperglycemia mitigated function impairment mice. Pathological analysis revealed that improved tissue damage, reduced fibrosis, diminished glycogen deposition. Transcriptomic indicated influenced mRNA CYP450-related genes, including Cyp2j13 , Cyp2b9 Pla2 g2e /Cyp4a12a Cyp4a32 Cyp2e1 Cyp4a14 . Non-targeted results demonstrated altered levels metabolites associated AA metabolic 5,6-EET, 14,15-EET, phosphatidylcholine, 20-HETE. Further experiments showed upregulated CYP4A CYP2E proteins tissue, CYP2J CYP2B proteins. enhanced antioxidant enzyme activity, alleviated CONCLUSION exerts anti-inflammatory regulating CYP450-mediated metabolism, leading state

Language: Английский

Citations

0
Untargeted metabolomics revealed that quercetin improves rat renal metabolic disorders induced by chronic unpredictable mild stress DOI
Yali Hou,

Yaru Li,

Jian Li

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

0
The Impact of Various Carbon Sources on Lipid Production in Cultivated Micractinium pusillum and the Evaluation of Antioxidant and Antiproliferative Properties of the Most Omega-3-Rich Lipid Extract DOI
Mostafa M. El‐Sheekh, Heba M. Mansour, Mohamed Y. Bedaiwy

et al.

Chemistry Africa, Journal Year: 2025, Volume and Issue: 8(4), P. 1377 - 1389

Published: March 5, 2025

Language: Английский

Citations

0
Gut microbiota regulates oxidative stress and inflammation: a double-edged sword in renal fibrosis DOI Creative Commons

Xiaojun Li,

Qiyuan Shan, X. Wu

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Dec. 1, 2024

Gut microbiota is a complex and dynamic system that plays critical roles in human health various disease. Progressive chronic kidney disease (CKD) suggests patients irreversibly progress to end-stage need renal replacement treatments, including dialysis transplantation. Ample evidence indicates local oxidative stress inflammation play pivotal the pathogenesis progression of CKD dysbiosis gut microbiota. always accompanied by intestinal stress, which lead rapid systemic translocation bacterial-derived uraemic toxins, indoxyl sulphate, phenyl sulphate indole-3-acetic acid, consequent development aggravation fibrosis. Although have been extensively discussed, there paucity reports on effects fibrosis mediation inflammation. This review provides an overview fibrosis, briefly discusses regulation flora using microecological preparations natural products, such as resveratrol, curcumin emodin treatments for CKD, clear pathophysiological rationale design promising therapeutic strategies.

Language: Английский

Citations

2
Effects of Salmonella Typhimurium Infection on Intestinal Flora and Arachidonic Acid Metabolism in Wenchang Chickens DOI Open Access

Shenghong Chen,

Yaochen Xie,

Dingqian Guo

et al.

Published: April 17, 2024

Salmonella infections can lead to intestinal inflammation and metabolic disorders in birds. However, whether ARA metabolism is involved Salmonella-induced remains unclear. This experiment investigated the changes cecal flora arachidonic acid (ARA) Hainan Wenchang chickens infected with S. Typhimurium using 16s rDNA sequencing targeted metabolomics. The results showed that levels of metabolites were increased cecum tissue after infection Typhimurium, including prostaglandin E2, F2α, lipoxin A4, ±8(9)-EET, ±11(12)-EET, ±8,9-DiHETrE. content key enzymes for production (PLA2 COX-2) chicken tissues was infection. relative mRNA inflammatory factors also infection, IFN-γ, TGF-β1, IL-4, IL-6. In HD11 cells, use a cyclooxygenase (COX) inhibitor reduced COX-2 PGF2α induced by effectively response. addition, number beneficial genera (e.g., Bifidobacterium, Lactobacillus, Odorobacterium) significantly Typhimurium. this study, we revealed structure demonstrated mediate onset development through activation pathway chickens. provide data support theoretical prevention control avian salmonellosis.

Language: Английский

Citations

1
Metabolic Chaos in Kidney Disease: Unraveling Energy Dysregulation DOI Open Access
Priya Gupta,

Saiya Zhu,

Yuan Gui

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(22), P. 6772 - 6772

Published: Nov. 11, 2024

Acute kidney injury (AKI) and chronic disease (CKD) share a fundamental disruption: metabolic dysfunction.

Language: Английский

Citations

0
Carthamus tinctorius L. protects cerebral ischemia/reperfusion injury via arachidonic acid/p53-mediated apoptosis axis DOI Creative Commons
Junren Chen,

Liujun Wu,

Xiaofang Xie

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 24, 2024

Introduction Stroke is a debilitating disease and the second leading cause of death worldwide, which ischemic stroke dominant type. Carthamus tinctorius L., also known as safflower, has been used to treat cerebrovascular diseases, especially in many Asian countries. However, underlying mechanisms safflower preventing remains elusive. This study aims elucidate potential drug candidate for prevention delineate its protective effects rat model cerebral ischemia-reperfusion injury (CI/RI). Methods The aqueous extract (AESF) was verified using HPLC-UV, HPLC-MS, TLC. inhibitory effect AESF on platelet aggregation detected vitro zebrafish mice. A CI/RI rats established by middle artery occlusion reperfusion stroke. 2,3,5-triphenyltetrazolium chloride, hematoxylin eosin, Nissl’s staining were employed evaluate pathological changes brain tissue. In addition, metabolomics, ELISA, Western blot uncover molecular alteration induced AESF. Results significantly inhibited , reduced thrombogenesis zebrafish, prolonged clotting time alleviated neurological dysfunction, oedema, infarct size, histopathological damage ischemia-reperfusion, improved neuronal survival, increased serum levels SOD CAT, decreased iNOS NO. Metabolomics revealed that attenuated metabolic disturbances caused I/R via regulating 38 metabolites particularly related arachidonic acid (AA) metabolism. Moreover, elevated 6-keto-PGF 1α pivotal metabolite AA, downregulated protein expression p53, Bax, cleaved caspase-9, caspase-3, caspase-8, upregulated Bcl-2. Conclusion mitigated through aggregation, alleviating oxidative stress, suppressing apoptosis partially modulating AA metabolism/p53-mediated axis.

Language: Английский

Citations

0