Causal relationship between immune cells, metabolites and polycystic ovary syndrome identified by Mendelian randomization and mediation analyses

Immunology and Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Immune cells and blood metabolites play essential roles in the development of polycystic ovary syndrome (PCOS); however, it remains unclear whether mediate causal relationship between immune PCOS. This study aimed to delineate relationships among cells, PCOS potential through Mendelian randomization (MR). A two-sample MR analysis was conducted using inverse variance weighting as primary method determine causation risk. supplemented by a two-step assess mediating role In addition, series sensitivity methods were employed test robustness results. We also performed reverse evaluate possibility relationships. Our findings identified 22 cell phenotypes causally linked PCOS, with 12 acting risk factors 10 protective for Furthermore, 45 or ratios related Mediation revealed that X-25519 levels mediated 9.2% absolute count CD28-CD25++ CD8br N-acetylglucosamine/n-acetylgalactosamine adenosine 5'-monophosphate 6.7% -11.1%, respectively, naive DN(CD4- CD8-) %T The aspartate-to-citrate ratio 8.6% CD20- CD38- %B Finally, studies did not identify any elucidates links highlighting four interaction thus providing new targets research therapeutic interventions.

Language: Английский

Causal Relationship Between Hypothyroidism and Multiple Cardiovascular Diseases: A Bidirectional Mendelian Randomization Study

International Journal of Clinical Practice, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Objective: Hypothyroidism is associated with a variety of cardiovascular diseases, but whether they are causally related to each other unclear. This study intends explore the bidirectional causal relationship between hypothyroidism and four common diseases using Mendelian randomization (MR). Methods: Single‐nucleotide polymorphisms (SNPs), instrumental variable required for study, were obtained from public genome‐wide association (GWAS) database, samples European populations. Causal effects assessed three methods: inverse variance weighted (IVW), MR‐Egger regression, median (WM), IVW being main method. Sensitivity analyses conducted MR‐PRESSO, Cochran’s Q test, regression intercept term, leave‐one‐out method assess reliability stability results. Results: The results showed effect on coronary heart disease (CHD) (OR: 2.313, 95% CI: 1.271 4.208, p = 0.006) hypertension 2.293, 1.331 3.955, 0.003). In contrast, there was no arrhythmias 1.448, 0.885 2.371, 0.141) failure 1.512, 0.775 2.951, 0.226). MR‐PRESSO test that heterogeneity in variables included CHD, while hypertension; also, term none horizontally pleiotropic. reverse MR analysis any hypothyroidism. Conclusion: Patients more likely develop CHD as well hypertension.

Language: Английский

Cathepsin B: Plasma Expression and Concentration in Non-Hodgkin Lymphoma Patients

Hemato, Journal Year: 2025, Volume and Issue: 6(2), P. 13 - 13

Published: May 6, 2025

Numerous studies point to the significance of cathepsin B (CTSB) in development carcinoma. Therefore, aim this pilot study was investigate levels and expression CTSB mRNA plasma non-Hodgkin lymphoma (NHL) patients. Methods: The included 44 newly diagnosed NHL patients 35 healthy volunteers comprising control group. samples were detected using enzyme-linked immunosorbent assay (ELISA). Results: level significantly higher compared subjects: 15.28 (11.68–17.23) versus 11.57 (10.12–13.41), p = 0.003. In addition, a positive correlation between after therapy observed (rho 0.591, 0.026). Regarding redox parameters, we found negative total antioxidant status (TAS) −0.499, 0.035), as well with oxidant (TOS) 0.576, 0.012). Conclusions: Targeting might have significant clinical relevance diagnostics NHL.

Language: Английский