Effect of nHA/CS/PLGA delivering adipose stem cell-derived exosomes and bone marrow stem cells on bone healing—in vitro and in vivo studies DOI Creative Commons
Ting Wang, Shu Guo, Ye Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 11, 2024

Adipose stem cell-derived exosomes (ADSC-EXO) have been demonstrated to promote osteogenic differentiation of bone marrow cells (BMSCs) and facilitate regeneration. The present study aims investigate the effect ADSC-EXO-loaded nano-hydroxyapatite/chitosan/poly-lactide-co-glycolide (nHA/CS/PLGA) scaffolds on maxillofacial regeneration using tissue engineering. ADSC-EXO was isolated co-cultured with BMSCs, BMSCs assessed through detection mineralized nodule formation, alkaline phosphatase (ALP) activity, mRNA expression COL1A1 runt-related transcription factor 2 (RUNX2). nHA/CS/PLGA were fabricated loaded these engineering complexes applied defect region rabbits elucidate their effect. markedly enhanced when they ADSC-EXO. This evidenced by an increase in formation ALP In vivo experiments that application effectively repaired defects rabbits. has BMSCs. shown defects. may serve as a potential therapeutic strategy for large-scale

Language: Английский

Osteoprotective Activity of Sambucus javanica Reinw Ex Blume subsp. javanica Leaf Extracts by Suppressing ROS Production DOI Creative Commons
Treethip Sukkho, Chartchai Khanongnuch, Saisamorn Lumyong

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(3), P. 252 - 252

Published: Feb. 21, 2025

Sambucus javanica subsp. (SJ) has been used in traditional medicine the northern region of Thailand for healing bone fractures; however, studies on how this plant stimulates formation are still scarce. The present study aimed to investigate potential crude extracts and fractions obtained from SJ leaves osteoporotic protection. All samples were investigated murine preosteoblast MC3T3-E1 cells resorption biomarkers, namely alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), receptor activator nuclear factor-κB ligand (RANKL), OPG/RANKL ratio. Additionally, calcium deposits determined using alizarin red S staining technique. results indicated that water ethanol contained gallic acid, rutin, chlorogenic acid as major compounds. stimulated osteoblastic cell differentiation enhanced osteoprotective activity, measured by a significant increase ALP OC, OPG, ratio, degree calcification. they exhibited negative impact significantly reducing RANKL reactive oxygen species (ROS) production. Therefore, our findings add novel evidence indicating extraction could be further utilized natural active pharmaceutical ingredient (NAPI) development health products.

Language: Английский

Citations

0
Coffee and tea consumption on the risk of osteoporosis: a meta-analysis DOI Creative Commons
W. Li, Y. G. Xie, Lei Jiang

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: March 4, 2025

Objectives This meta-analysis aims to quantify the relationship between coffee and tea consumption risk of osteoporosis explore whether such positively or negatively impacts this risk, thereby providing a scientific basis for understanding effects on bone health. Methods We systematically searched PubMed, Cochrane Library, Embase observational studies published up November 5, 2024, using medical subject headings (MeSH) keywords related “osteoporosis, tea, coffee.” Statistical analyses were conducted Stata software version 14.0. A fixed-effects model was used when heterogeneity low ( I 2 ≤ 50% p > 0.1). random-effects greater 50%). Publication bias assessed funnel plots Egger’s regression tests. Results included 14 comprising 562,838 participants 2008 2024. The pooled analysis showed that is significantly associated with reduced (odds ratio [OR] = 0.79, 95% confidence interval [CI]: 0.73–0.84, 28.9%, < 0.05). Tea also demonstrated protective effect, lower (OR 0.75, CI: 0.62–0.91, 80.4%, Subgroup revealed high-frequency (more than one cup per day) reduction in 0.83, 0.74–0.93, 0.001) compared low-frequency (less day), which no statistically significant 0.86, 0.68–1.07, 0.171). Similarly, four times week) exhibited slightly stronger effect against 0.82, 0.70–0.97, 0.02). Conclusion suggests long-term osteoporosis. Moreover, higher frequency within moderate range appeared enhance Systematic review registration https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024612101 , PROSPERO CRD42024612101.

Language: Английский

Citations

0
Hydroethanolic extract of Spondias mombin L. leaves attenuates alveolar bone loss and inflammation in a model of periodontitis induced in male Wistar rats DOI

Ana Beatriz Costa Almeida,

Agnes Andrade Martins, Susana Barbosa Ribeiro

et al.

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106249 - 106249

Published: March 1, 2025

Language: Английский

Citations

0
Effects of Eucommia ulmoides leaf extracts and caltrate on osteoblasts proliferation and differentiation DOI
Xuan Ma,

Dongli Yin,

Tingting Liu

et al.

Bioactive Carbohydrates and Dietary Fibre, Journal Year: 2024, Volume and Issue: 32, P. 100448 - 100448

Published: Aug. 30, 2024

Language: Английский

Citations

0
Immuno‐Isolation Strategy with Tacrolimus‐Loaded Nanofilm Promotes Stable Stem Cell‐Based Cartilage Regeneration DOI

Zhiyi Guo,

Xinsheng Zhu,

Liang Xu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Abstract Bone marrow stem cells (BMSCs)‐engineered cartilage (BEC) shows promise for clinically repairing defects. However, when implanted in immunocompetent large animals, BEC becomes susceptible to ossification due inflammatory infiltration. To address this, a nanofilm isolation approach is developed enhance BEC's chondrogenic stability. Tacrolimus (FK506), known its immunosuppressive effect, integrated into adipic dihydrazide (ADH)‐modified hyaluronic acid (HA), creating an acid‐responsive macromolecular prodrug called FK506@HA‐ADH. This then blended with poly(lactic‐co‐glycolic acid) (PLGA) form electrospun FK506@HA/PLGA nanofilm. Goat‐derived BMSCs are induced vitro BEC, which enclosed within the and subcutaneously autologous goats. The acted as physical barrier, preventing immunocyte Additionally, response acidic environment triggered by inflammation gradual degradation of PLGA, FK506@HA‐ADH cleaved, releasing FK506 needed. released effectively countered cytokines promoted cartilaginous maturity. These combined mechanisms significantly inhibited hypertrophy improved stability goat model. nanofilm‐based strategy established niche, successfully endochondral promoting stable formation BEC. advancements crucial translating cell‐based therapies clinical use repair.

Language: Английский

Citations

0
Effect of nHA/CS/PLGA delivering adipose stem cell-derived exosomes and bone marrow stem cells on bone healing—in vitro and in vivo studies DOI Creative Commons
Ting Wang, Shu Guo, Ye Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 11, 2024

Adipose stem cell-derived exosomes (ADSC-EXO) have been demonstrated to promote osteogenic differentiation of bone marrow cells (BMSCs) and facilitate regeneration. The present study aims investigate the effect ADSC-EXO-loaded nano-hydroxyapatite/chitosan/poly-lactide-co-glycolide (nHA/CS/PLGA) scaffolds on maxillofacial regeneration using tissue engineering. ADSC-EXO was isolated co-cultured with BMSCs, BMSCs assessed through detection mineralized nodule formation, alkaline phosphatase (ALP) activity, mRNA expression COL1A1 runt-related transcription factor 2 (RUNX2). nHA/CS/PLGA were fabricated loaded these engineering complexes applied defect region rabbits elucidate their effect. markedly enhanced when they ADSC-EXO. This evidenced by an increase in formation ALP In vivo experiments that application effectively repaired defects rabbits. has BMSCs. shown defects. may serve as a potential therapeutic strategy for large-scale

Language: Английский

Citations

0