Rhus coriaria (Sumac) induces autophagic cell death and inhibits mTOR, p38MAPK and STAT3 pathways in 5fluorouracil-resistant colorectal cancer cells DOI Creative Commons
Zohra Nausheen Nizami,

Mazoun Al Azzani,

Soumaya Khaldi

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Introduction Colorectal cancer is a leading cause of related-death worldwide, and resistance to 5-fluorouracil (5FU, key component chemotherapy regimens, major clinical concern. We have previously elucidated the effects Rhus coriaria ethanolic extract (RCE) in triple-negative breast cancer, CRC, pancreatic cells. Here, we explored anticancer RCE parental (HCT-116-WT) 5FU-resistant HCT-116 (HCT-116-5FU-R) CRC Methods MTT assay was used assess cell viability. Muse analyzer viability, cycle distribution, apoptosis. Additionally, colony formation growth assays western blots were performed. In vivo assessed by an ovo chick embryo tumor assay. Results found that inhibited viability capacities HCT-116-WT HCT-116-5FU-R The antiproliferative attributed DNA damage-mediated impairment at S phase, induction Beclin-1-independent autophagy both lines. Mechanistically, inhibition mTOR, STAT3 p38 MAPK pathways implicated latter. induced caspase-7-independent apoptosis However, cells resistant through upregulation survivin, downregulation Bax. Using proteasome inhibitors, clarified pathway contributed RCE-mediated death Lastly, confirmed xenografts model. Discussion Collectively, our findings highlight source phytochemicals can be as agents for CRC.

Language: Английский

Rhus coriaria (Sumac) induces autophagic cell death and inhibits mTOR, p38MAPK and STAT3 pathways in 5fluorouracil-resistant colorectal cancer cells DOI Creative Commons
Zohra Nausheen Nizami,

Mazoun Al Azzani,

Soumaya Khaldi

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

Introduction Colorectal cancer is a leading cause of related-death worldwide, and resistance to 5-fluorouracil (5FU, key component chemotherapy regimens, major clinical concern. We have previously elucidated the effects Rhus coriaria ethanolic extract (RCE) in triple-negative breast cancer, CRC, pancreatic cells. Here, we explored anticancer RCE parental (HCT-116-WT) 5FU-resistant HCT-116 (HCT-116-5FU-R) CRC Methods MTT assay was used assess cell viability. Muse analyzer viability, cycle distribution, apoptosis. Additionally, colony formation growth assays western blots were performed. In vivo assessed by an ovo chick embryo tumor assay. Results found that inhibited viability capacities HCT-116-WT HCT-116-5FU-R The antiproliferative attributed DNA damage-mediated impairment at S phase, induction Beclin-1-independent autophagy both lines. Mechanistically, inhibition mTOR, STAT3 p38 MAPK pathways implicated latter. induced caspase-7-independent apoptosis However, cells resistant through upregulation survivin, downregulation Bax. Using proteasome inhibitors, clarified pathway contributed RCE-mediated death Lastly, confirmed xenografts model. Discussion Collectively, our findings highlight source phytochemicals can be as agents for CRC.

Language: Английский

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