Therapeutic Potential of Carbon Dots Derived from Phytochemicals as Nanozymes Exhibiting Superoxide Dismutase Activity for Anemia

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Anemia is a potentially life-threatening blood disorder caused by an insufficient erythroblast volume in the circulatory system. Self-renewal failure of progenitors one key pathological factors leading to deficiency. However, there are currently no effective drugs that selectively target this process. In work, we present carbon dot (CP-CDs) derived from phytochemicals significantly promotes self-renewal for anemia therapy. As superoxide dismutase (SOD)-like nanozyme, CP-CDs activate hypoxia response and JAK/STAT3 pathways erythroid cells reprogramming oxidative stress state. This results unique erythropoiesis-enhancing properties promoting generation progenitor cells. Moreover, protect mature red inhibiting stress-induced damage improving immune-inflammatory microenvironment. vivo, showed promising therapeutic effect mouse zebrafish models with minimal adverse effects, indicating significant translational medical value. Collectively, study not only illustrates successful approach nanomedicine-enhanced therapy but also enhances our understanding interaction between nanomedicine progenitors.

Language: Английский

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Transcriptomic and Metabolomic Analyses Provide Insights Into the Flavonoid Biosynthesis in Dangshen

Phytochemical Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

ABSTRACT Introduction Dangshen (DS) has been used for hundreds of years as a traditional Chinese medicine. It wide range biological activities. Flavonoids are one the important bioactive components with strong free radical scavenging and antioxidant capacity in DS. However, biosynthesis process flavonoids DS remains unclear. Objective The aim this study was to understand molecular mechanism Methods In study, metabolomics research transcriptome sequencing were carried out. Transcript metabolite profiles generated by high‐throughput RNA (RNA‐seq) data analysis liquid chromatography–tandem mass spectrometry, respectively. Results total, 256 metabolites identified root, stem, leaf, flower using untargeted metabolomics. Among them, 55 flavonoids, including pinobanksin, butein, fustin, pelargonidin, apigenin, luteolin, eriodictyol, closely related flavonoid metabolism, most them upregulated different tissues Furthermore, differentially expressed genes transcriptomics mainly enriched flavonoid, isoflavonoid, flavone, flavonol. A number genes, ANS, CCOAOMT, CHI, CHS, CYP75B1, CYP75A, CYP93B2_16, CYP98A/C3'H, DFR, F3H, FLS, HCT, may regulate production An integrated metabolome revealed biosynthetic network elucidated diversity pathway roots, stems, leaves, flowers Conclusion Our findings provide basis new insights into lay foundation breeding valuable cultivars.

Language: Английский

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Adaptogens in Long-Lasting Brain Fatigue: An Insight from Systems Biology and Network Pharmacology

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 261 - 261

Published: Feb. 15, 2025

Long-lasting brain fatigue is a consequence of stroke or traumatic injury associated with emotional, psychological, and physical overload, distress in hypertension, atherosclerosis, viral infection, aging-related chronic low-grade inflammatory disorders. The pathogenesis linked to disrupted neurotransmission, the glutamate-glutamine cycle imbalance, glucose metabolism, ATP energy supply, which are multiple molecular targets signaling pathways neuroendocrine-immune blood circulation systems. Regeneration damaged tissue long-lasting multistage process, including spontaneously regulating hypothalamus-pituitary (HPA) axis-controlled anabolic-catabolic homeostasis recover harmonized sympathoadrenal system (SAS)-mediated function, deregulated gene expression rehabilitation. driving mechanism spontaneous recovery regeneration cross-talk mediators neuronal, microglia, immunocompetent, endothelial cells collectively involved neurogenesis angiogenesis, plant adaptogens can target. Adaptogens small molecules origin that increase adaptability organisms stress by interaction HPA axis SAS (neuroendocrine-immune cardiovascular complex), targeting adaptive GPCR pathways. Two major groups comprise (i) phenolic phenethyl phenylpropanoid derivatives (ii) tetracyclic pentacyclic glycosides, whose chemical structure be distinguished as related correspondingly monoamine neurotransmitters (epinephrine, norepinephrine, dopamine) steroid hormones (cortisol, testosterone, estradiol). In this narrative review, we discuss multitarget integrated pharmacological activity botanical ischemic stroke, fatigue; time-dependent dual response physiological regulatory systems support overload; (iii) dose-dependent reversal (hormetic) effect adaptogens. This review shows adaptogenic concept cannot reduced rectified various effects on selected specific modes action without estimating their interactions within networks complex that, turn, regulates other (cardiovascular, gastrointestinal, reproductive systems) due numerous intra- extracellular communications feedback regulations. These result polyvalent pleiotropic adaptogens, essential for characterizing distinct types botanicals. They trigger defense leads extension limits resilience inducing mental For first time, justifies potential particularly hybrid preparation (BHP) Arctic Root Ashwagandha, providing rationale use individuals experiencing fatigue. provided insight into future research network pharmacology preventing rehabilitating following trauma, infections.

Language: Английский

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Natural Products Alleviate Atrial Fibrillation by Modulating Mitochondrial Quality Control

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156555 - 156555

Published: Feb. 22, 2025

Language: Английский

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Advances in Modern Medical Research on the Hematopoietic Effect of Qi and Blood Replenishing Drugs

Medical Diagnosis, Journal Year: 2025, Volume and Issue: 15(01), P. 123 - 133

Published: Jan. 1, 2025

Language: Английский

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An inulin-type fructan CP-A from Codonopsis pilosula combined with 5-Fluorouracil alleviates colitis-associated tumorigenesis via inhibition of EGFR/AKT/ERK signaling pathway and regulation of intestinal flora

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142655 - 142655

Published: March 1, 2025

Language: Английский

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The mechanistic study of codonopsis pilosula on laryngeal squamous cell carcinoma based on network pharmacology and experimental validation

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Introduction Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck, with poor prognosis for advanced patients, there an urgent need to find new treatment strategies. Codonopsis pilosula, traditional Chinese medicinal herb, possesses various pharmacological activities, but its antitumor effects mechanisms in LSCC are still unclear. The aim this study was systematically investigate potential mechanism pilosula LSCC. Methods In study, we screened effective compounds targets by TCMSP, ETCM BATMAN-TCM databases, related combining DisGeNET, GeneCards database Cytoscape software. KEGG pathway enrichment analysis utilized explore signaling pathways. core were further based on TCGA GEO analysis, molecular docking carried out predict their binding ability compounds. presence key verified LC-MS, MAPK3 expression detected qPCR tissues, knockdown proliferation, migration, invasion, cycle, apoptosis cells evaluated cellular function assays. Results 22 that might regulate network pharmacology. showed pilosula-LSCC mainly involved HIF-1, TNF, IL-17 FoxO Based identified as target pilosula-LSCC. results variety from had strong abilities MAPK3, among them, Caprylic Acid, Emodin Luteolin have been confirmed LC-MS. QPCR indicated highly expressed tissues. significantly inhibits migration invasion. It also suppresses growth blocking cycle inducing apoptosis. Conclusion exerts through regulation multiple pathways, providing theoretical basis clinical application.

Language: Английский

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Modified Zhenwu Decoction improved intestinal barrier function of experimental colitis through activation of sGC-mediated cGMP/PKG signaling

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 334, P. 118570 - 118570

Published: July 11, 2024

The invasion of luminal antigens and an aberrant immune response resulting from a disrupted physical epithelial barrier are the key characteristics ulcerative colitis (UC). restoration damaged function is crucial for maintaining mucosal homeostasis disease quiescence. Current therapies UC primarily focus on suppressing inflammation. However, most patients fail to respond therapy or develop secondary resistance over time, emphasizing need novel therapeutic targets UC. Our study aimed identify potential modified herbal formula Zhen Wu Decoction, namely CDD-2103, which has demonstrated promising efficacy in treating chronic colitis.

Language: Английский

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Bacillus licheniformisYB06: A Rhizosphere–Genome-Wide Analysis and Plant Growth-Promoting Analysis of a Plant Growth-Promoting Rhizobacterium Isolated from Codonopsis pilosula

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1861 - 1861

Published: Sept. 8, 2024

Codonopsis pilosula, commonly known as Dangshen, is a valuable medicinal plant, but its slow growth and susceptibility to environmental stress pose challenges for cultivation. In pursuit of sustainable agricultural practices enhance the yield quality present study isolated bacterial strain exhibiting plant growth-promoting potential from rhizosphere C. pilosula. This was subsequently identified Bacillus licheniformisYB06. Assessment attributes revealed B. licheniformis YB06 biofertilizer. Whole-genome sequencing genome size 4,226,888 bp with GC content 46.22%, harboring 4325 predicted protein-coding sequences. Genomic analysis diverse array genes linked induced systemic resistance (ISR) (PGP) traits, encompassing phytohormone production, nitrogen assimilation reduction, siderophore biosynthesis, phosphate solubilization, biofilm formation, synthesis PGP-related amino acids, flagellar motility. Seed germination assays demonstrated positive effects on pilosula seedlings. Furthermore, we explored various fertilization regimes, particularly YB06-based biofertilizer, were investigated their impact structure diversity soil community. Our findings that significantly impacted composition diversity, combined application biofertilizer organic fertilizer pronounced enhancement community diversity. represents first report beneficial both microbial These provide theoretical foundation practical guidance development novel bio-organic compound fertilizers, thereby contributing cultivation

Language: Английский

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“Qi-Invigoration” Action of <em>Codonopsis pilosula</em> Polysaccharide Through Altering Mitochondrial Bioenergetics

Published: Oct. 28, 2024

Background: Codonopsis pilosula (CP) is one of the most popular Qi-invigorating herbal medicines and has been extensively used to promote health vitality in China for a long time. C. polysaccharide (CPP) principal active components CP, which considered as reason CP widespread application. However, there are not revealed that CPP exert Qi-invigoration effect by protecting mitochondria and/or improving mitochondrial function existing traditional Chinese medicine theories. Methods: Herein, we extracted from investigated effects on energy metabolism protection. Based mice chronic hypoxia model imitating &ldquo;Qi&rdquo; deficiency state human body medicine, was administered with oral gavage daily 10 days, permeability transition (MPT), lipid peroxidation product malondialdehyde (MDA) brain, respiratory function, levels adenosine triphosphate (ATP), diphosphate (ADP) monophosphate (AMP) liver cells were assayed. Adenylate charge (AEC), total adenylate pool (TAP), ATP/ADP, ATP/AMP ratios calculated. Results: could inhibit formation MDA brains, decrease oxygen consuming rate control ratio (RCR) mitochondria, increase ATP, TAP AEC under condition. Conclusion: Overall, can invigorate bioenergetic levels.

Language: Английский

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