Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer DOI Creative Commons

Xing Guo,

Rui‐Sheng Wang, Zhenling Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Introduction Non-small cell lung cancer (NSCLC) is a prominent disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become research hotspot because of its safe effective characteristics. The branches leaves Taxus media are abundant in antitumor active compounds, there been no conducted yet regarding anti–lung molecular mechanism. Objective aim this study to investigate the activity two samples before after fermentation T. , mechanism inhibitory effect on NSCLC. Methods chemical composition pre-fermentation (TM) post-fermentation qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS high-performance liquid chromatography (HPLC). anti-lung activities TM TMQ compared an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin eosin (H&E) staining, immunohistochemistry, immunofluorescence determine action. Results results indicated that contained 83 consisting primarily flavonoids, organic acids, taxanes. Both taxanes flavonoids higher than TM. effectively inhibited growth non-small (NSCLC), inhibition rate was greater (57.24%) (49.62%). administration downregulated necrosis factor-α (TNF-α), interleukin-6 (IL-6), glutathione (GSH) level upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), malondialdehyde (MDA) levels serum mice. treatment also increased protein expression Bax, Caspase-3, Beclin-1 tissues. In contrast, bcl-2, PI3K, Ki67, ULK1, mTOR suppressed by TMQ. Protein analyses reemphasized superior over These cumulative findings demonstrated action closely related activation transcriptional misregulation pathway cholinergic synaptic, AMPK, PI3K/Akt/mTOR signaling pathways. Conclusion This ingredient contents effects . addition, herbal for NSCLC treatment.

Language: Английский

Phytochemical diversity and biological activities of Hypericum japonicum and Hypericum sampsonii: Potential for natural product-based food applications DOI Creative Commons
Paula Barciela, Daniele Bobrowski Rodrigues, Ana Perez-Vazquez

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 484, P. 144355 - 144355

Published: April 15, 2025

Language: Английский

Citations

0
Seasonal Chemical Variability and Antimicrobial, Anti-Proliferative Potential of Essential Oils from Baccharis uncinella, B. retusa, and B. calvescens (Asteraceae) DOI Creative Commons

Tânia F. Dlugoviet,

Áurea Portes Ferriani, Ana Paula P. Klein Hendges

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1311 - 1311

Published: April 26, 2025

Essential oils (EOs) of three native species Baccharis uncinella, B. retusa and calvescens, obtained through hydrodistillation, were analyzed by GC-MS GC-FID for seasonality, the antimicrobial anti-proliferative activities evaluated. EO calvescens uncinella consisted mainly oxygenated sesquiterpenes, while in retusa, monoterpene hydrocarbons predominant. The highest activity was observed spring against S. aureus, C. albicans summer subtilis. showed more effective than EO. This is first study it demonstrated that majority composition different all seasons year, justifying importance seasonal study. Furthermore, potent cytostatic effects K562 OVCAR-03 cell lines, respectively. For each species, PCA differentiated chemical seasonally. samples distinguished species. underscores assessing variation biological essential oils, highlighting potential compounds spathulenol, caryophyllene oxide, limonene α-pinene achieving desired product properties.

Language: Английский

Citations

0
Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer DOI Creative Commons

Xing Guo,

Rui‐Sheng Wang, Zhenling Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Introduction Non-small cell lung cancer (NSCLC) is a prominent disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become research hotspot because of its safe effective characteristics. The branches leaves Taxus media are abundant in antitumor active compounds, there been no conducted yet regarding anti–lung molecular mechanism. Objective aim this study to investigate the activity two samples before after fermentation T. , mechanism inhibitory effect on NSCLC. Methods chemical composition pre-fermentation (TM) post-fermentation qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS high-performance liquid chromatography (HPLC). anti-lung activities TM TMQ compared an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin eosin (H&E) staining, immunohistochemistry, immunofluorescence determine action. Results results indicated that contained 83 consisting primarily flavonoids, organic acids, taxanes. Both taxanes flavonoids higher than TM. effectively inhibited growth non-small (NSCLC), inhibition rate was greater (57.24%) (49.62%). administration downregulated necrosis factor-α (TNF-α), interleukin-6 (IL-6), glutathione (GSH) level upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), malondialdehyde (MDA) levels serum mice. treatment also increased protein expression Bax, Caspase-3, Beclin-1 tissues. In contrast, bcl-2, PI3K, Ki67, ULK1, mTOR suppressed by TMQ. Protein analyses reemphasized superior over These cumulative findings demonstrated action closely related activation transcriptional misregulation pathway cholinergic synaptic, AMPK, PI3K/Akt/mTOR signaling pathways. Conclusion This ingredient contents effects . addition, herbal for NSCLC treatment.

Language: Английский

Citations

0