Cited by The interaction of isoquinoline alkaloid crebanine with immunoglobulin G and cytotoxic effects toward MCF-7 breast cancer cell line

Transcriptome Analysis of Stephania yunnanensis and Functional Validation of CYP80s Involved in Benzylisoquinoline Alkaloid Biosynthesis DOI

Wenlong Shi,

Qishuang Li, Xinyi Li

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(2), P. 259 - 259

Published: Jan. 10, 2025

The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloid (BIA), with being the representative and most abundant compound, but our understanding biosynthesis BIAs this has been relatively limited. Previous research reported genome S. preliminarily identified norcoclaurine synthase (NCS), which involved early stages BIA biosynthetic pathways. However, key genes promoting formation skeleton have not yet reported. In study, based on differences content crebanine several other different tissues, we conducted transcriptome sequencing roots, stems, leaves. We then candidate through functional annotation sequence alignment further analyzed them combination genome. Based analysis, three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, SyCYP80G6), exhibited activities toward (S)- (R)-configured substrates demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic transcriptomic information yunnanensis, offers valuable insights into elucidation biosynthesis, lays foundation for complete analysis pathways more alkaloids.

Language: Английский

Citations

Transcriptome analysis of Stephania yunnanensis and functional validation of CYP80s involved in benzylisoquinoline alkaloids biosynthesis DOI

Wenlong Shi,

Qishuang Li, Xinyi Li

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract The medicinal plant Stephania yunnanensis is rich in aporphine alkaloids, a type of benzylisoquinoline alkaloids (BIAs), with being the representative and most abundant compound, but our understanding on biosynthesis BIA this have been relatively limited. Previous research has reported genome S. preliminarily identified upstream gene norcoclaurine synthase (NCS) biosynthetic pathways. However, key genes promoting formation skeleton not yet reported. In study, based differences content crebanine several other BIAs different tissues, we conducted transcriptome sequencing roots, stems, leaves. We then candidate through functional annotation sequence alignment, followed by transcriptomic genomic analyses. Based analysis, three CYP80 enzymes (SyCYP80Q5-1, SyCYP80Q5-3, SyCYP80G6), which exhibited activities towards (S)- (R)-configured substrates yunnanensisand demonstrated strict stereoselectivity enroute to aporphine. This study provides metabolomic information offers valuable insights into elucidation biosynthesis, lays foundation for complete analysis pathways more alkaloids.

Language: Английский

Citations

Targeting glioma with heteroaromatic alkaloids: A review of potential therapeutics DOI

Karen Ichikawa,

H. Johnson, Maurice A. Curtis

et al.

Bioorganic & Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 121, P. 118051 - 118051

Published: Dec. 25, 2024

Glioblastoma multiforme (GBM), classified as a grade IV astrocytoma, is the most aggressive and deadly form of glioma, characterized by rapid progression, extensive genetic heterogeneity, resistance to conventional therapies. Despite advancements in surgical techniques, radiation therapy, frontline chemotherapeutic agent temozolomide, prognosis for GBM patients remains poor, with median survival 15 months 5-year rate approximately 7 %. The absence effective long-term treatments underscores urgent, unmet clinical need novel therapeutic strategies improve patient outcomes. Natural products, particularly alkaloids, have garnered attention rich source bioactive compounds diverse pharmacological properties. Alkaloids, structurally group natural are renowned their properties ability cross blood-brain barrier (BBB), making them promising candidates glioma therapy. This review systematically examines all reported heteroaromatic alkaloids documented anti-glioma activities, highlighting mechanisms action where available. By providing comprehensive resource, it aims facilitate identification optimisation alkaloid-based glioma-targeted drug discovery. Additionally, this emphasizes importance incorporating products into development pipeline address pressing challenges associated treatment.

Language: Английский

Citations

The interaction of isoquinoline alkaloid crebanine with immunoglobulin G and cytotoxic effects toward MCF-7 breast cancer cell line DOI

Shaorong Zhao,

Lu Yang, Wenzhu Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139194 - 139194

Published: Dec. 1, 2024

Language: Английский

Citations