Electroacupuncture and Tongbian decoction ameliorate CUMS-induced depression and constipation in mice via TPH2/5-HT pathway of the gut-brain axis

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: 221, P. 111207 - 111207

Published: Jan. 14, 2025

Depression is commonly associated with gastrointestinal (GI) disorders, such as constipation, which can potentially intensify depressive symptoms. The interplay between these conditions believed to be facilitated by the gut-brain axis, suggests a complex bidirectional interaction. Current treatments, antidepressants and prokinetics, are often side effects high recurrence rates, highlighting need for effective treatments targeting both depression constipation. This study was designed assess therapeutic efficacy of electroacupuncture (EA) in conjunction Tongbian decoction (TB) management while also investigating underlying mechanisms through lens axis. Chronic unpredictable mild stress (CUMS) employed induce comorbidity model constipation mice, followed administration EA, EA + TB, fluoxetine (FLX). findings demonstrated that antidepressant combination were more pronounced than those alone. TB treatment significantly ameliorated anxiety-like behaviors, restored cognitive function, enhanced motility chronic mice. Furthermore, reduced intestinal inflammation, neuronal morphology, increased expression tryptophan hydroxylase 2 (TPH2) prefrontal cortex (PFC) colon, elevated serum levels 5-hydroxytryptophan (5-HTP)-a molecule acts connector-and promoted synthesis production serotonin (5-HT) tract brain. Contrastingly, FLX showed limited improving In conclusion, regulates TPH2/5-HT pathway via demonstrating synergistic regulation nervous systems, favorable prokinetic effects.

Language: Английский

Antioxidant and Photoprotective Activities of 3,4-Dihydroxybenzoic Acid and (+)-Catechin, Identified from Schima argentea Extract, in UVB-Irradiated HaCaT Cells

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 241 - 241

Published: Feb. 19, 2025

In traditional Chinese medicine, the root bark and leaves of Schima argentea are utilized to treat dysentery, parasitic infections, digestive disorders. this study, n-butanol extract S. (NBA) exhibited potent antioxidant properties, protecting HaCaT cells from UVB-induced damage, was abundant in phenolic flavonoid compounds. Using UPLC-QTOF-MS analysis, several antioxidants within NBA were identified. Among these, 3,4-dihydroxybenzoic acid, (+)-catechin, procyanidin B2 effectively reduced ROS levels after 1 h post-UVB treatment (225 mJ/cm2). Notably, all three compounds significantly decreased phosphorylation p38 JNK a dose-dependent manner. Additionally, cell survival rate these assessed 12 Both acid (+)-catechin prevented apoptosis cells, as evidenced by MTT, Hoechst, Calcein/PI staining, flow cytometry analyses. Proteomic analysis revealed that achieved photoprotection downregulating c-Fos Jun modulating cycle proteins, while promoted repair through PI3K-Akt Wnt signaling pathways. These results demonstrated both can directly absorb UVB, scavenge ROS, provide multiple The holds promising potential for future medical applications.

Language: Английский

Regulation of macrophage polarization and pyroptosis by 4-methylcatechol alleviates collagen-induced arthritis via Nrf2/HO-1 and NF-κB/NLRP3 signaling pathways

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113855 - 113855

Published: Dec. 21, 2024

Language: Английский