Exploring 5,7-dimethoxyflavone from Piper ornatum as a novel anti-breast cancer candidate: insights from in silico analysis DOI Creative Commons
Aulia Umi Rohmatika, Turan Demircan, Arniza Khairani Mohd Jamil

et al.

Brazilian Journal of Biology, Journal Year: 2025, Volume and Issue: 85

Published: Jan. 1, 2025

This study investigates the potential of compound 5,7-dimethoxyflavone (5,7-DMF) found in Piper ornatum as an anti-breast cancer agent using silico approach. The targeted three major mechanisms involved breast progression: ability 5,7-DMF to inhibit kinase activity, act a competitive inhibitor cyclooxygenase 2 (COX-2) and nitric oxide synthase (NOS2). was investigated through molecular simulation by assessing drug-likeness, toxicity, membrane permeability, bioactivity, specific docking with AutoDock Vina integrated PyRx 8.0, well dynamics CABS-flex 2.0. Molecular results showed that has high binding affinity towards COX-2 target inhibits activity. also revealed significant interactions between active site, supporting its agent. These provide solid basis for further development drug candidate against cancer.

Language: Английский

Cytotoxic Effects of Citrus Peels on Breast Tumor: Opportunities for Waste to Raw Material Conversion DOI Creative Commons
Ömer Faruk Yakıncı, Esra Emerce, Perihan Gürbüz

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: 10(16), P. 16900 - 16908

Published: April 17, 2025

Citrus species have long been known for their rich nutritional value. Recent research has shed light on therapeutic potential, particularly in cancer treatment. peels, the other hand, often discarded as waste, contain a wealth of bioactive compounds, such flavonoids, coumarins, and essential oil components, which proven medicinal properties. Converting peels from waste products to raw materials is crucial approach both healthcare sustainability. Therefore, present study aims investigate cytotoxic potential aurantium L., maxima (Burm.) Merr. (syn. grandis), medica L. paradisi Macfad. cultivated Türkiye, find out compounds responsible activity. The effects peel extracts were evaluated MCF-7 cell lines according bioactivity-guided fractionation isolation assay procedures. CAS-5 (isomeranzin), CAS-10 (3-methoxy nobiletin), CAS-11 (nobiletin), CAS-12 (tangeretin) isolated. In silico analyses conducted isolated provided supporting information results obtained vitro experiments regarding anticancer Indeed, one key components fruits polymethoxy flavonoids (PMFs), group constituents recognized anti-inflammatory, antioxidant, activities. As valuable byproduct PMFs offer dual benefit by reducing while providing natural source making them an exciting area management. promise lies not only ability combat but also contribute sustainable practices.

Language: Английский

Citations

0

Systematic review on effectiveness of flavonoids against hypercholesterolemia: Insights from in-silico, in-vitro, and in-vivo studies DOI

Nurnazira Hanis,

Nurul Azira Ismail, Ernie Zuraida Ali

et al.

Food Chemistry Advances, Journal Year: 2025, Volume and Issue: 7, P. 100981 - 100981

Published: April 21, 2025

Language: Английский

Citations

0

2-Amino-3-Chlorobenzoic Acid from Streptomyces coelicolor: A Cancer Antagonist Targeting PI3K/AKT Markers via miRNA Modulation DOI Creative Commons
Ashraf Khalifa,

Joseph Devadass Balthazar,

Pandurangan Subash‐Babu

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 620 - 620

Published: April 24, 2025

Background/Objectives: Actinomycetes, particularly species within the Streptomyces genus, are renowned for their ability to produce a wide array of bioactive molecules with therapeutic potential. This study aimed comprehensively investigate antimicrobial and anticancer properties coelicolor ERI-15, particular focus on purified compound, 2-amino-3-chlorobenzoic acid (2A3CB), its efficacy against microbial pathogens breast cancer cell lines. Methods: Antimicrobial compounds were produced through fermentation techniques isolated via column chromatography. Bioassay-guided fractionation was conducted Staphylococcus aureus (ATCC 25923), methicillin-resistant (MRSA), Escherichia coli 25922), Bacillus subtilis 441). Major fractions further using preparative thin-layer chromatography (TLC). The structures active elucidated spectral analyses including IR, mass spectrometry, 1H/13C NMR. compound 2A3CB (m/z 171) tested MDA-MB-231 3T3 Cytotoxicity assessed by MTT assay, apoptotic mechanisms explored proliferation assays, dual fluorescent staining, migration invasion analysis markers at mRNA protein levels. Results: exhibited strong cytotoxic effects cells, IC50 values 26 µM, 5 7.2 µM 24, 48, 72 h, respectively. It significantly inhibited migration, induced apoptosis caspase-mediated pathways. Expression levels PTEN, PCNA, BAX, STAT3 downregulated, suggesting inhibition metastasis suppression migration. Conclusions: results demonstrate that 2A3CB, derived from S. possesses potent properties. Its inhibit growth induce in cells highlights potential as natural candidate targeted treatment, progression.

Language: Английский

Citations

0

Importance of Advanced Detection Methodologies from Plant Cells to Human Microsystems Targeting Anticancer Applications DOI Open Access
Mostafa Gouda, Eman Ramadan Elsharkawy, Yong He

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4691 - 4691

Published: May 14, 2025

The growing global demand for phytochemicals as bioactive sources is prompting scientists to develop methods that link their sensory properties mechanisms of action in cancer treatment. Recent techniques tracking the actions small plant metabolites (SPMs) from single-cell molecular anticancer biomarkers could provide valuable insights this field. Among critical discussed review are real-time cell components through stable isotope probing (Sis) and microspectroscopy, which has attracted attention biotechnologists. Additionally, precise pathways required studying new into functional materials discussed, based on high-resolution accurate technologies, aid categorization. Notably, molecules under study have recently garnered applications due advancements effective evaluation surpass traditional methods. In December 2020, Food Drug Administration (FDA) authorized 89 SPMs safe natural molecules. conclusion, by combining spatiotemporal SPMs' mechanisms, they facilitate development more exceptional, bio-efficient materials.

Language: Английский

Citations

0

Effects of Grape By-Products on Oxidative Stress and Inflammation in Farm Animals: An Overview of Studies Performed in Pigs, Chickens, and Cattle DOI Creative Commons
Klaus Eder, Robert Ringseis, Denise K. Geßner

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(11), P. 1536 - 1536

Published: May 23, 2025

High-yielding farm animals often face severe metabolic stress, compounded by environmental stressors such as psychosocial heat intensive housing systems, and poor hygiene management. These factors result in oxidative stress inflammatory processes, which adversely affect both animal health performance. Polyphenols are known to alleviate responses. Since grapes rich polyphenols, by-products of winemaking could have beneficial effects on these processes. This review aims provide an overview the potential antioxidative anti-inflammatory grape animals. The first section examines causes consequences inflammation. second highlights general polyphenols addressing issues. third central part presents findings from studies investigating impact various grape-derived antioxidant system inflammation pigs, chicken, cattle. Overall, demonstrate that can effectively reduce pigs chickens, leading improved In cattle, however, fewer been conducted, results regarding less consistent. conclusion, represent valuable feed options for preventing monogastric (pigs, chickens).

Language: Английский

Citations

0

Potential Therapeutic Efficacy of Ferulic Acid and Its Derivatives in the Management of Cancers: A Comprehensive Analysis With Mechanistic Insight DOI Creative Commons
Shakil Ahmmed, Md. Shimul Bhuia, Raihan Chowdhury

et al.

International Journal of Food Science, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Due to the increasing rate of cancer and undesirable consequences manufactured drugs, there is a growing interest in development natural products as potential remedies. Ferulic acid (FA), phenolic substance, found naturally present Ferula foetida plant’s cell walls with therapeutic activities. The objective this study determine botanical sources, pharmacokinetics, anticancer activity FA its derivatives, focusing on molecular mechanism by using data obtained from literature database. study’s findings suggest that demonstrates promising effects preclinical pharmacological test methods. exhibit showed through underlying mechanisms, including induction oxidative stress, cytotoxic effect, cycle arrest, apoptotic suppression invasion migration, antiproliferative autophagy, genotoxic mutagenic effect regulating different pathways like PI3K/AKT, p38/MAPK/ERK, AKT/mTOR, NF‐ κ B signaling which are involved growth. Additionally, review indicated pharmacokinetic properties FA, indicating lower oral bioavailability affected liver’s fast conjugation process; limitation overcome applying nanoformulation FA. However, additional clinical investigations recommended appropriate effectiveness, safety, human dosage.

Language: Английский

Citations

0

Exploring 5,7-dimethoxyflavone from Piper ornatum as a novel anti-breast cancer candidate: insights from in silico analysis DOI Creative Commons
Aulia Umi Rohmatika, Turan Demircan, Arniza Khairani Mohd Jamil

et al.

Brazilian Journal of Biology, Journal Year: 2025, Volume and Issue: 85

Published: Jan. 1, 2025

This study investigates the potential of compound 5,7-dimethoxyflavone (5,7-DMF) found in Piper ornatum as an anti-breast cancer agent using silico approach. The targeted three major mechanisms involved breast progression: ability 5,7-DMF to inhibit kinase activity, act a competitive inhibitor cyclooxygenase 2 (COX-2) and nitric oxide synthase (NOS2). was investigated through molecular simulation by assessing drug-likeness, toxicity, membrane permeability, bioactivity, specific docking with AutoDock Vina integrated PyRx 8.0, well dynamics CABS-flex 2.0. Molecular results showed that has high binding affinity towards COX-2 target inhibits activity. also revealed significant interactions between active site, supporting its agent. These provide solid basis for further development drug candidate against cancer.

Language: Английский

Citations

0