Orco mediates olfactory behavior and oviposition in the whitefly Bemisia tabaci

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 199, P. 105773 - 105773

Published: Jan. 21, 2024

Language: Английский

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Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs

BMC Genomics, Journal Year: 2021, Volume and Issue: 22(1)

Published: Dec. 13, 2021

Abstract Background Insecticide-treated bed nets and indoor residual spraying comprise the major control measures against Anopheles gambiae sl , dominant vector in sub-Saharan Africa. The primary site of contact with insecticide is through mosquitoes’ legs, which represents first barrier insecticides have to bypass reach their neuronal targets. Proteomic changes leg cuticle modifications been associated resistance that may reduce rate penetration insecticides. Here, we performed a multiple transcriptomic analyses focusing on An. coluzzii legs. Results Firstly, leg-specific enrichment analysis identified 359 genes including pyrethroid-binder SAP2 2 other chemosensory proteins, along 4 ABCG transporters previously shown be enriched. Enrichment gene families included those involved detecting chemical stimuli, gustatory ionotropic receptors implicated hydrocarbon-synthesis. Subsequently, compared transcript expression legs highly resistant strain (VK7-HR) both very similar genetic background has reverted susceptibility after several generations without pressure (VK7-LR) lab susceptible population (NG). Two hundred thirty-two differentially expressed (73 up-regulated 159 down-regulated) were when two counterparts, indicating an over-expression phase I detoxification enzymes cuticular decrease hormone-related metabolic processes from population. Finally, analysed short-term effect pyrethroid exposure comparing 1 h-deltamethrin-exposed (VK7-IN) unexposed mosquitoes 348 encoding for GPCRs, ABC transporters, odorant-binding proteins members divergent salivary gland protein family. Conclusions data transcriptome provides valuable insights into line defense deltamethrin-exposed mosquitoes. Our results suggest xenobiotic likely occurring while sensory also evident. Constitutive primarily elevated levels genes, insecticide-induced tolerance linked overexpression GPCRs GPCR-related sensory/binding proteins.

Language: Английский

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A sublethal dose of neonicotinoid imidacloprid precisely sensed and detoxified by a C-minus odorant-binding protein 17 highly expressed in the legs of Apis cerana

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 885, P. 163762 - 163762

Published: May 3, 2023

Language: Английский

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An odorant receptor tuned to an attractive plant volatile vanillin in Spodoptera litura

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 196, P. 105619 - 105619

Published: Sept. 13, 2023

Language: Английский

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Identification of candidate leg chemosensory genes associated with nursing behavior in the Apis mellifera

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2025, Volume and Issue: unknown, P. 101540 - 101540

Published: May 1, 2025

Language: Английский

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Functional characterization of sensory neuron membrane protein 1a involved in sex pheromone detection of Apolygus lucorum (Hemiptera: Miridae)

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: 23(12), P. 4120 - 4135

Published: March 11, 2024

The mirid bug Apolygus lucorum (Hemiptera: Miridae) is a polyphagous pest that affects wide range of host plants. Its control remains challenging mainly due to its rapid reproduction, necessitating an understanding sex pheromone communication. recognition pheromones vital for courtship and mating behaviors, mediated by various chemosensory-associated proteins. Among these, sensory neuron membrane protein (SNMP), CD36-related protein, suggested play crucial roles in detecting pheromones. In this study, we employed transcriptomic genomic data from A. phylogenetic approaches, identified four putative SNMP genes (AlucSNMP1a, AlucSNMP1b, AlucSNMP2a, AlucSNMP2b) with full open reading frames. Expression analysis revealed the ubiquitous presence AlucSNMP transcripts multiple tissues, only AlucSNMP1a exhibiting male-biased expression antennae, suggesting potential role male chemosensation. Functional using Xenopus oocyte system, coupled two-electrode voltage clamp recording, demonstrated co-expression specific receptors (PRs) co-receptor (Orco) significantly enhanced electrophysiological responses compared PRs Orco alone. Moreover, results indicated not affected responsiveness but also influenced kinetics (activation inactivation) induced signals. contrast, AlucSNMP1b AlucPR/Orco complexes had no impact on inward currents two compounds. An examination selective pressures SNMP1 across 20 species strong purifying selection, implying functional conservation insects. These findings highlight response

Language: Английский

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Morphological and genetic differences in legs of a polygamous beetle between sexes, Glenea cantor (Coleopter: Cerambycidae: Lamiinae)

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(2), P. e0297365 - e0297365

Published: Feb. 8, 2024

The legs of insects play an important role in their daily behaviour, especially reproduction. Entomologists have performed much research on the leg different behaviours beetles, group insect family, but relatively little has been done to study ultrastructure and transcriptome legs. Hence, we systematically studied gene expression G . cantor , a polygynous beetle, compared its male female diversity. In this study, found fore-leg, mid-leg hind-leg were significantly longer than those male. From perspective intuitive structural differences, also ultrastructures adhesion structure (tarsal) males females. tarsal functional adult mainly includes sensilla structure. joint include chaetica (SCh II, SCh III) trichodea (ST II). disc-shaped bristles (di), lanceolate (la), serrated (se), spatula-shaped (spl) mushroom-shaped (mus). Although there was no significant difference sensillum distribution or type between females, differences species structures mid-leg, same sex Therefore, roles various beetles. On other hand, results screened for subset olfaction- mechanics-related genes. We discovered that showed upregulation 1 odorant binding protein (OBP), 2 Olfactory receptors (ORs) Chemosensory proteins (CSPs). Meanwhile, 3 OBPs, OR, Gustatory receptor (GR) Mechanosensitive (MSPs). An in-depth examination molecular composition can elucidate function reproductive behavior Moremore, investigation will serve as cornerstone subsequent into underlying behavioral mechanisms.

Language: Английский

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Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

Journal of Insect Science, Journal Year: 2020, Volume and Issue: 20(5)

Published: Aug. 4, 2020

Abstract The small white butterfly, Pieris rapae (L.), is an important insect pest of Brassica crops. This species utilize olfactory cues to find their hosts and mates. However, the molecular mechanism underlying perception in this remains unclear. Here, we identified 14 odorant-binding proteins (OBP) genes—essential for olfaction—in P. by exploring a previously published transcriptome dataset. Proteins encoded all these genes contain N-terminal signal peptides six positionally conserved cysteine residues, which are characteristic OBPs. These OBPs displayed high amino acid identity with respective orthologs other lepidopterans, several motifs were within Phylogenetic analysis showed that well segregated from each clustered into different branches. PrapOBP1 PrapOBP2 ‘general protein’ clade, PrapOBP3 PrapOBP4 fall ‘pheromone-binding clade. OBP located on seven genomic scaffolds. Of these, PrapOBP1, 2, 3, 4 scaffold332, whereas PrapOBP5, 6, 7, 8, 9 scaffold116. Ten had antenna-biased expression. 4, 13 enriched male antennae, PrapOBP7 PrapOBP10 female-biased. Our findings suggest may be involved communication. To best our knowledge, first report identification characterization rapae, provide solid foundation studying functions genes.

Language: Английский

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Genome-wide identification and expression pattern analysis of novel chemosensory genes in the German cockroach Blattella germanica

Genomics, Journal Year: 2022, Volume and Issue: 114(2), P. 110310 - 110310

Published: Feb. 11, 2022

The German cockroach Blattella germanica is an important urban insect pest worldwide. In many insects, chemosensation essential for guiding their behaviors survival. Although a large number of chemosensory-related genes have been identified in B. germanica, little information on tissue-specific and developmental expression patterns has not uncovered yet. this study, we performed transcriptome analysis different tissues to reveal novel chemosensory proteins (CSPs) sensory neuron membrane (SNMPs). addition, phylogenetic tree gender-specific multiple gene families analyzed. We three CSPs (BgerCSP11, BgerCSP12, BgerCSP13) five SNMP germanica. Tissue-specific profiling showed that CSP1, 8, 9 exhibited significant levels both adult 5th instar nymph antennae. results paved the way further functional study mechanism provided potential insecticide targets.

Language: Английский

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Identification and functional analysis of odorant-binding proteins provide new control strategies for Apolygus lucorum

International Journal of Biological Macromolecules, Journal Year: 2022, Volume and Issue: 224, P. 1129 - 1141

Published: Oct. 24, 2022

The green bug Apolygus lucorum is a notorious pest that feeds on multiple crops, including fruit trees, vegetables, and cotton. odorant-binding proteins (OBPs) are considered to perform crucial roles in regulating A. behaviors such as mating feeding. In this study, we first identified OBPs the genome. Then, calculated expression levels of these OBP genes different tissues stages. Thereafter, conducted ligand-binding assay test interactions between nine selected AlucOBPs chemical compounds. result showed there were 31 encoding 39 transcripts genome, several clusters found. Comprehensive profiling revealed tissue-specific some genes. results fluorescence competitive binding assays could specifically bind plant volatiles, nonvolatile compounds, synthetic analogs thereof. Additionally, AlucOBP19 was suggested function gustatory sensing avoid deleterious secondary metabolites, high mouthparts legs interact with quercetin. Our findings highlight potential biotechnological application volatiles their ecological attractants provide new gene targets for control lucorum.

Language: Английский

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