Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 329 - 350
Published: Nov. 3, 2023
Language: Английский
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 27, 2025
The Philippines is a renowned marine biodiversity hotspot, home to several sea cucumber species with unusual biological traits. Among these, Stichopus cf. horrens notable for its ability undergo rapid body wall liquefaction when stressed, coupled remarkable regenerative abilities. In contrast, Holothuria scabra has one of the most robust walls in cucumbers and thrives many regimes tropics. Despite their intriguing traits, neurobiology chemical diversity these remain underexplored. Neuropeptides are important components an animal's neurobiological toolkit that underlie various physiological behavioral processes. Thus, discovery neuropeptides crucial step understanding molecular underpinnings unique traits cucumbers. Leveraging throughput sensitivity tandem mass spectrometry, we obtained unbiased view endogenous peptidomes radial nerve cord tissues non-model species, H. S. horrens. this work, sequenced 60 mature peptides from were derived 22 precursor proteins, 43 originating 25 proteins nervous tissues. A total seven previously unannotated uncharacterized neuropeptide precursors identified, thereby expanding known animal repertoire. Furthermore, discovered consistent structural features based on type post-translational modifications while pushing forward potentially novel proteolytic processing sites during peptide maturation enriched flanking amino acid residues. Collectively, our results provide preliminary data expand echinoderm through discovery, paving way innovative solutions address global demand echinoderms.
Language: Английский
Citations
0
Aquaculture, Journal Year: 2025, Volume and Issue: 603, P. 742366 - 742366
Published: Feb. 28, 2025
Language: Английский
Citations
0
Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742432 - 742432
Published: March 1, 2025
Language: Английский
Citations
0
General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 114716 - 114716
Published: March 1, 2025
Language: Английский
Citations
0
BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)
Published: May 8, 2025
Asian water buffaloes (Bubalus bubalis) in the Yangtze River Basin of China are important meat provider for local residents because its outstanding body size. Several previous studies have highlighted their genetic basis growth characteristics, but crucial genes regulating traits via multi-layer omics still rarely investigated. We conducted a comprehensive multi-omics analysis integrating blood and muscle transcriptome, plasma metabolome, rumen fluid metagenome, genome Haizi buffaloes. Of note, ribosomal protein L26 (RPL26) located evolutionary selection regions associated with sizes is top differentially expressed gene (DEG) both tissues. Further metabolomics metagenomics identified growth-related molecular biomarkers (myristicin Bacteroidales) microbiological composition (Bacteroides Prevotella). Leveraging cattle quantitative trait loci (QTLs) genotype-tissue expression (CattleGTEx) databases, we found significant correlations QTL_180979 on RPL26 two cis-eQTLs tissue upstream weight gain. The follow-up cell assay validations confirmed regulation roles cycle, apoptosis, differentiation, where low expressions enhanced antiapoptotic ability promoted differentiation myoblasts into myotubes markedly. Our study illustrates integrated functional that suggests further applications
Language: Английский
Citations
0
Marine Drugs, Journal Year: 2022, Volume and Issue: 20(5), P. 320 - 320
Published: May 12, 2022
Today, marine natural products are considered one of the main sources compounds for drug development. Starfish and sea cucumbers potential pharmaceutical interest. Among their metabolites, polar steroids, triterpene glycosides, lipids have attracted a great deal attention; however, studying these by conventional methods is challenging. The application modern MS-based approaches can help to obtain valuable information about such compounds. This review provides an up-to-date overview applications starfish cucumber bioactive analysis. While describing most characteristic features in context including sample preparation MS analysis steps, present paper mainly focuses on metabolic profiling steroid compounds, lipids. metabolomics studies also outlined.
Language: Английский
Citations
14
Aquaculture, Journal Year: 2023, Volume and Issue: 579, P. 740150 - 740150
Published: Sept. 24, 2023
Language: Английский
Citations
8
Biology, Journal Year: 2022, Volume and Issue: 11(10), P. 1538 - 1538
Published: Oct. 20, 2022
Here, we described the complex nervous system at five early developmental stages (blastula, gastrula, auricularia, doliolaria and pentactula) of a holothurian species with highly economic value, Apostichopus japonicus. The results revealed that embryos larvae is mainly distributed in anterior apical region, ciliary bands or rings, feeding attachment organs, serotonergic immunoreactivity was not observed until embryo developed into late gastrula; these are evolutionarily conserved features echinoderm, hemichordate protostome larvae. Furthermore, based on available transcriptome data, reported neuropeptide precursors profile different embryonic larval stages. This analysis showed 40 present adult sea cucumbers were also identified larvae, only four (SWYG precursor 2, GYWKDLDNYVKAHKT precursor, Neuropeptide 14-like GLRFAmprecursor-like precursor) predicted adults absent Combining quantitative expression ten specific genes (NPs) by qRT-PCR, potential important roles neuropeptides development, A. japonicus In conclusion, this work provides novel perspectives diverse physiological functions contributes to understanding evolution neuropeptidergic systems echinoderm
Language: Английский
Citations
12
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(18), P. 14358 - 14358
Published: Sept. 20, 2023
Vasopressin/oxytocin (VP/OT)-type neuropeptide is an ancient neurophysin-associated and has been intensively studied to be involved in multiple physiological processes protostomian deuterostome vertebrates. However, little known about the functions of VP/OT-type invertebrates especially echinoderms. Here, we firstly report signaling important economic species, Apostichopus japonicus, which widely cultured Asia, with high nutritional medicinal values. Molecular characterization analysis holotocin its precursor revealed highly conserved features VP/OT family. The candidate receptor for (AjHOR) was confirmed able activate via cAMP-PKA possible Ca2+-PKC pathway, further activated downstream ERK1/2 cascade. Holotocin expression profile showed that they were mainly concentrated circumoral nerve ring. Furthermore, vitro pharmacological experiments demonstrated caused contractile responses preparations from A. japonicus. And vivo functional studies indicated short-term injection resulted body bloat long-term reduced mass, suggesting potential roles osmoregulation feeding co-inhibition holotocin-CCK. Our findings provided a comprehensive description AjHOR-holotocin signaling, inhibition by controlling muscle contractions.
Language: Английский
Citations
7
Aquaculture, Journal Year: 2021, Volume and Issue: 543, P. 736960 - 736960
Published: May 25, 2021
Language: Английский
Citations
10