Roadbumps at the Crossroads of Integrating Behavioral and In Vitro Approaches for Neurotoxicity Assessment DOI Creative Commons
G. Jean Harry, Sandra J. McBride,

Shannah K. Witchey

et al.

Frontiers in Toxicology, Journal Year: 2022, Volume and Issue: 4

Published: Feb. 25, 2022

With the appreciation that behavior represents integration and complexity of nervous system, neurobehavioral phenotyping assessment has seen a renaissance over last couple decades, resulting in robust database on rodent performance within various testing paradigms, possible associations with human disorders, therapeutic interventions. The interchange data across other test modalities multiple model systems advanced our understanding fundamental biology mechanisms associated normal functions alterations system. While there is demonstrated value power assessments for examining due to genetic manipulations, maternal factors, early development environment, applied use assess environmental neurotoxicity continues come under question as whether sensitive endpoint assessment. Why tool neuroscientist yet, not when used pre-clinical or chemical studies? Applying new paradigms evidence biological basis requires expertise refinement how such experiments are conducted minimize variability maximize information. This review presents relevant issues methods conduct test, sources variability, experimental design, analysis, interpretation, reporting. It beneficial critical limitations they translate vivo environment considers need integrate disciplines best value. proposes behavioral subtle pronounced differences will facilitate obtained approaches address translation.

Language: Английский

Engineering Human Brain Assembloids by Microfluidics DOI
Yujuan Zhu, Xiaoxuan Zhang, Lingyu Sun

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(14)

Published: Jan. 12, 2023

Brain assembloids offer a highly promising strategy to model human brain development and disease, advance potential studies in regenerative medicine, therapeutic screening, drug discovery, while it is challenging produce uniform organoids assemble them flexibly by conventional methods. Here, multidisciplinary engineered generate with desired patterning based on microfluidic technology presented. By encapsulating induced pluripotent stem cells microcapsules via electrospray, region-specific are efficiently formed, which then introduced into chip consisting of bottom layer micropillar array movable upper complementary microhole array. These can settle microholes fuse assembloids. As varied organoid designed 1D sequences or 2D arrays be assembled the vertical microholes, large coding amounts fused produced. It found that composed cortical, hippocampal, thalamic grow function well, characterized active neural migration interaction. features indicate suggested flexible, scalable, controlled systems remarkably wide applications neurological biomedical fields.

Language: Английский

Citations

68
Organoids meet microfluidics: recent advancements, challenges, and future of organoids-on-chip DOI

Talha Chauhdari,

S. A. A. Zaidi,

Jilei Su

et al.

In vitro models, Journal Year: 2025, Volume and Issue: 4(1), P. 71 - 88

Published: March 5, 2025

Language: Английский

Citations

2
Harnessing the power of artificial intelligence for human living organoid research DOI Creative Commons
Hui Wang, Xiangyang Li, Xiaoyan You

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 140 - 164

Published: Aug. 30, 2024

As a powerful paradigm, artificial intelligence (AI) is rapidly impacting every aspect of our day-to-day life and scientific research through interdisciplinary transformations. Living human organoids (LOs) have great potential for

Language: Английский

Citations

11
The Application of Brain Organoids in Assessing Neural Toxicity DOI Creative Commons
Fan Pan,

YuanHao Wang,

Min Xu

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: Feb. 9, 2022

The human brain is a complicated and precisely organized organ. Exogenous chemicals, such as pollutants, drugs, industrial may affect the biological processes of or its function eventually lead to neurological diseases. Animal models not fully recapitulate for testing neural toxicity. Brain organoids with self-assembled three-dimensional (3D) structures provide opportunities generate relevant tests predictions neurotoxicity. In this study, we reviewed recent advances in organoid techniques their application assessing toxicants. We hope review provides new insights further progress screening studies

Language: Английский

Citations

34
Extracellular Vesicles and the Stress System DOI Open Access

Evanthia A. Makrygianni,

George P. Chrousos

Neuroendocrinology, Journal Year: 2022, Volume and Issue: 113(2), P. 120 - 167

Published: Sept. 22, 2022

Extracellular vesicles (EVs) are membrane-enclosed nanoparticles that contain various biomolecules, including nucleic acids, proteins and lipids, manufactured released by virtually all cell types. There is evidence EVs involved in intercellular communication, acting an autocrine, paracrine or/and endocrine manner. the cells of central nervous system (CNS), neurons, astrocytes, oligodendrocytes microglia, have ability to cross blood-brain barrier (BBB) enter systemic circulation. Neuroendocrine specialized neurons secrete hormones directly into blood vessels, such as hypophyseal portal or circulation, a process allows neuroendocrine integration take place. In mammals, widely distributed throughout anatomic compartments, with hypothalamus being integrator. The key part stress (SS), highly conserved neuronal/neuroendocrine aiming at maintaining homeostasis when latter threatened stressors. parts SS interconnected hypothalamic corticotropin-releasing hormone (CRH) brainstem locus caeruleus-norepinephrine (LC-NE) systems, while their peripheral are, respectively, pituitary-adrenal axis sympathetic nervous/sympatho-adrenomedullary systems (SNS-SAM) well components parasympathetic (PSNS). During stress, multiple CNS loci show plasticity undergo remodeling, partly mediated increased glutamatergic noradrenergic activity, actions cytokines glucocorticoids, regulated interaction hypothalamic-pituitary-adrenal (HPA) LC-NE/SNS-SAM systems. addition, there changes due secretion pro-inflammatory context stress-related (para)inflammation. We speculate during peripheral, cellular molecular alterations place, some them generated, communicated, spread via release stress-induced neural/neuroendocrine cell-derived EVs.

Language: Английский

Citations

30
3D multicellular systems in disease modelling: From organoids to organ-on-chip DOI Creative Commons
Caoimhe Goldrick, Ina Guri,

Gabriel Herrera-Oropeza

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 2, 2023

Cell-cell interactions underlay organ formation and function during homeostasis. Changes in communication between cells their surrounding microenvironment are a feature of numerous human diseases, including metabolic disease neurological disorders. In the past decade, cross-disciplinary research has been conducted to engineer novel synthetic multicellular systems 3D, organoids, assembloids, organ-on-chip models. These model systems, composed distinct cell types, satisfy need for better understanding complex biological mechanisms underpinning diseases. this review, we discuss emerging field building 3D application modelling cellular at play We report recent experimental computational approaches capturing cell-cell as well progress bioengineering recapitulating these complexities

Language: Английский

Citations

20
Advanced lung organoids for respiratory system and pulmonary disease modeling DOI Creative Commons

Hyebin Joo,

Sungjin Min, Seung‐Woo Cho

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell and animal models. Nevertheless, organoids still face limitations concerning heterogeneity, complexity, maturity compared to native tissue. To address these limitations, researchers have employed co-culture methods with various types including endothelial cells, mesenchymal immune incorporated bioengineering platforms air-liquid interfaces, microfluidic chips, functional hydrogels. These advancements facilitated applications studies pulmonary diseases, providing insights into mechanisms potential treatments. This review introduces progress in production organoids, strategies for improving maturity, functionality, complexity their application modeling, infection fibrosis.

Language: Английский

Citations

7
Recent trends in neuromorphic systems for non-von Neumann in materia computing and cognitive functionalities DOI
Indrajit Mondal, Rohit Attri, Tejaswini S. Rao

et al.

Applied Physics Reviews, Journal Year: 2024, Volume and Issue: 11(4)

Published: Oct. 1, 2024

In the era of artificial intelligence and smart automated systems, quest for efficient data processing has driven exploration into neuromorphic aiming to replicate brain functionality complex cognitive actions. This review assesses, based on recent literature, challenges progress in developing basic focusing “material-neuron” concepts, that integrate structural similarities, analog memory, retention, Hebbian learning brain, contrasting with conventional von Neumann architecture spiking circuits. We categorize these devices filamentary non-filamentary types, highlighting their ability mimic synaptic plasticity through external stimuli manipulation. Additionally, we emphasize importance heterogeneous neural content support conductance linearity, plasticity, volatility, enabling effective storage various types information. Our comprehensive approach categorizes fundamentally different under a generalized pattern dictated by driving parameters, namely, pulse number, amplitude, duration, interval, as well current compliance employed contain conducting pathways. also discuss hybridization protocols fabricating systems making use existing complementary metal oxide semiconductor technologies being practiced silicon foundries, which perhaps ensures smooth translation user interfacing new generation devices. The concludes outlining insights challenges, future directions realizing deployable field intelligence.

Language: Английский

Citations

7
Human mini brains and spinal cords in a dish: Modeling strategies, current challenges, and prospective advances DOI Creative Commons
Simeon Kofman, Neha Mohan, Xiaohuan Sun

et al.

Journal of Tissue Engineering, Journal Year: 2022, Volume and Issue: 13

Published: Jan. 1, 2022

Engineered three-dimensional (3D) in vitro and ex vivo neural tissues, also known as "mini brains spinal cords a dish," can be derived from different types of human stem cells via several differentiation protocols. In general, mini are micro-scale physiological systems consisting mixed populations progenitor cells, glial neurons that may represent key features brain anatomy function. To date, these specialized 3D tissue structures characterized into spheroids, organoids, assembloids, organ-on-a-chip their various combinations based on generation procedures cellular components. These CNS models incorporate complex cell-cell interactions play an essential role bridging the gap between two-dimensional neuroglial cultures animal models. Indeed, they provide innovative platform for disease modeling therapeutic cell replacement, especially shedding light potential to realize personalized medicine neurological disorders when combined with revolutionary induced pluripotent technology. this review, we highlight developed variety experimental strategies, emphasize advances remaining challenges, evaluate state-of-the-art applications recapitulating crucial phenotypic aspects many diseases, discuss contemporary technologies prospective improvement composition, consistency, complexity, maturation.

Language: Английский

Citations

25
A Multifunctional Acoustic Tweezer for Heterogenous Assembloids Patterning DOI Creative Commons
Zeping Gao, Shuo Wang, Yufei Sui

et al.

Small Structures, Journal Year: 2023, Volume and Issue: 4(5)

Published: March 3, 2023

Acoustic tweezers, capable of complicated manipulation bioparticles by acoustic radiation forces using a noninvasive and noncontact approach, are an innovative technology for patterning assemble organoids. Hence, tweezers exhibit considerable potential forming programmable organoids with specific spatial structures. Furthermore, heterogeneous assembloids complex arrangement patterns can be built through sequential assembling culturing to explore polarized tissue development or disease metastasis in multiple organs. This study focuses on the structural assembly ultrasonic 2D matrix array generate real‐time switching different fields. In addition, local renal injured assembloid is fabricated verify its application engineering modeling.

Language: Английский

Citations

15