A large effect fitness trade-off across environments is explained by a single mutation affecting cold acclimation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Abstract Identifying the genetic basis of local adaptation and fitness trade-offs across environments is a central goal evolutionary biology. Cold acclimation an adaptive plastic response for surviving seasonal freezing, costs may be general mechanism in temperate zone species. Starting with locally adapted ecotypes Arabidopsis thaliana from Italy Sweden, we examined consequences naturally occurring functional polymorphism CBF2 . This gene encodes transcription factor that major regulator cold-acclimated freezing tolerance, resides within locus responsible trade-off long-term mean fitness. We estimated alternate genotypes on 5-year components at native field sites by comparing near isogenic lines to their background ecotypes. The effects were validated nucleotide level using edited backgrounds grown simulated parental environments. foreign genotype reduced Sweden more than 10%, primarily via survival. In Italy, was 20%, fecundity. At both sites, temporally variable much stronger some years. confirmed causal variant underlying this trade-off. Additionally, demonstrated substantial cost cold acclimation, which has broad implications potential maladaptive responses climate change.

Language: Английский

Differential expression of hemolymph proteins in wild bumblebees provides insights into species‐specific impacts of heat stress

Physiological Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Wildlife faces an increasing threat from extreme climatic events, such as heatwaves, which can have a severe impact on various species, including crucial pollinators like bumblebees. Bumblebees are cold‐adapted and heterothermic, possessing the ability to regulate their internal temperature. The of heat stress seems species specific in While most impacted, some bumblebee manage survive, potentially by employing physiological mechanisms, modulation protein profile (e.g. Heat Shock Proteins). However, there is limited understanding how profiles associated with exposure. In this study, we examined global variation males two sampled wild: heat‐tolerant Bombus terrestris heat‐sensitive magnus . After subjecting them stupor at 40°C controlled condition, it was observed that nearly all B. survived stress, while over 50% individuals succumbed Through off‐gel bottom‐up proteomics LC–MS/MS analysis hemolymph proteome, identified 164 proteins both large part differentially expressed after Additionally, quantitative fat bodies revealed relative mass stable , significantly lower exposed stress. Our data suggest compared displays higher adaptability its proteome response This could be key factor contributing high resistance adapt new, stressful environments expected due climate change. Understanding these mechanisms regulation bumblebees provide valuable insights into resilience vulnerability facing environmental stresses.

Language: Английский