Endocrine and scavenging cell functions of a juvenile fish heart atrium: Fine structure aspects and translating conjectures DOI Creative Commons
Jacques Gilloteaux,

Lothar Jennes,

Jean‐Jacques Vanderhaeghen

et al.

Translational Research in Anatomy, Journal Year: 2023, Volume and Issue: 32, P. 100252 - 100252

Published: June 28, 2023

The atrium of the juvenile teleost, smallmouth bass, Micropterus dolomieu Lacépède showed a pigmented endocardium. Our goal was to use fine structure survey this fish heart tissues and verify content responsible for shrouding discuss mammalian, translational conjectures about cardiac structure. Using electron microscopy, pericardium, myocardium, endocardium were analyzed, including atrial peptide immunolabeling. endothelial cells revealed pinocytosis endocytosis activities that resulted in accumulated electron-contrasted secondary lysosomes lipofuscin bodies not found ventricle This endothelium contacted subjacent ventricular myocardial producing immunolabeled peptide-containing vesicles. Other migrating cells, melano-macrophages, noticed sub functioned as potent blood-heart barrier where transcytosis dispatching numerous materials, those peptides, between myocardium circulation occur. Peculiarly, abundant lysosomal processing scavenged materials like reticuloendothelial tissue. These cell's 'aging' organized deposits into network with other organelles, especially mitochondria without evident disposal. Additionally, melano-macrophages roamed both ventricle; also can influence viewed pigmentation. Some these data comforted endocardial mesodermal lineage, still sketchy all vertebrates while development is used. observations could raise questions: Does turnover associated captures make model mammalian pathologies, failure?

Language: Английский

The peroxisome: an update on mysteries 3.0 DOI Creative Commons

Rechal Kumar,

Markus Islinger, Harley L. Worthy

et al.

Histochemistry and Cell Biology, Journal Year: 2024, Volume and Issue: 161(2), P. 99 - 132

Published: Jan. 20, 2024

Language: Английский

Citations

20
Luteolin, a promising quorum quencher mitigates virulence factors production in Pseudomonas aeruginosa - In vitro and In vivoapproach DOI
S.P. Ramya Ranjan Nayak, Seenivasan Boopathi,

P. Snega Priya

et al.

Microbial Pathogenesis, Journal Year: 2023, Volume and Issue: 180, P. 106123 - 106123

Published: April 23, 2023

Language: Английский

Citations

25
Navigating sex and sex roles: deciphering sex-biased gene expression in a species with sex-role reversal ( Syngnathus typhle ) DOI Creative Commons
Freya Adele Pappert, Arseny Dubin, Guillermo G. Torres

et al.

Royal Society Open Science, Journal Year: 2024, Volume and Issue: 11(4)

Published: April 1, 2024

Sexual dimorphism, the divergence in morphological traits between males and females of same species, is often accompanied by sex-biased gene expression. However, majority research has focused on species with conventional sex roles, where have highest energy burden both egg production parental care, neglecting diversity reproductive roles found nature. We investigated expression

Language: Английский

Citations

4
Modelling Peroxisomal Disorders in Zebrafish DOI Creative Commons
C. Jiang, Michael Schrader

Cells, Journal Year: 2025, Volume and Issue: 14(2), P. 147 - 147

Published: Jan. 20, 2025

Peroxisomes are ubiquitous, dynamic, oxidative organelles with key functions in cellular lipid metabolism and redox homeostasis. They have been linked to healthy ageing, neurodegeneration, cancer, the combat of pathogens viruses, infection immune responses. Their biogenesis relies on several peroxins (encoded by PEX genes), which mediate matrix protein import, membrane assembly, peroxisome multiplication. Defects or peroxisomal enzymes can result severe disorders, including developmental neurological abnormalities. The drive understand role peroxisomes human health disease, as well their tissues organs during development, has led establishment vertebrate models. zebrafish (Danio rerio) become an attractive model organism investigate functions. Here, we provide overview visualisation zebrafish, metabolic inventory comparison peroxisomes. We then present models established disorders. These include for disorders/Zellweger Spectrum single enzyme deficiencies, particularly adrenoleukodystrophy fatty acid beta-oxidation Finally, highlight deficiencies dually targeted peroxisomal/mitochondrial proteins. Advantages investigation development approaches application drug screening discussed.

Language: Английский

Citations

0
The mitochondrial amidoxime reducing component—from prodrug-activation mechanism to drug-metabolizing enzyme and onward to drug target DOI
Michel A. Struwe, Axel J. Scheidig, Bernd Clement

et al.

Journal of Biological Chemistry, Journal Year: 2023, Volume and Issue: 299(11), P. 105306 - 105306

Published: Sept. 29, 2023

Language: Английский

Citations

8
OrganelX web server for sub-peroxisomal and sub-mitochondrial protein localization and peroxisomal target signal detection DOI Creative Commons
Marco Anteghini, Asmaa Haja, Vítor A. P. Martins dos Santos

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2022, Volume and Issue: 21, P. 128 - 133

Published: Dec. 5, 2022

We present the OrganelX e-Science Web Server that provides a user-friendly implementation of In-Pero and In-Mito classifiers for sub-peroxisomal sub-mitochondrial localization peroxisomal mitochondrial proteins Is-PTS1 algorithm detecting validating potential carrying PTS1 signal sequence. The is available at https://organelx.hpc.rug.nl/fasta/.

Language: Английский

Citations

5
Navigating sex and sex roles: deciphering sex-biased gene expression in a species with sex-role reversal (Syngnathus typhle) DOI Creative Commons
Freya Adele Pappert, Arseny Dubin, Guillermo Torres

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 2, 2023

Abstract Sexual dimorphism, the divergence in morphological traits between males and females of same species, is often accompanied by sex-biased gene expression. However, majority research has focused on species with conventional sex roles, where have highest energy burden both egg production parental care, neglecting diversity reproductive roles found nature. We investigated expression broadnosed pipefish ( Syngnathus typhle ), a sex-role reversed male pregnancy, allowing us to separate these two female traits. Employing RNA sequencing, we examined across organs (brain, head kidney, gonads) at various life stages, encompassing differences age, sex, status. While some groups were more strongly associated such as stress resistance immune defence, others driven biological lipid storage regulation an organ- age-specific manner. By investigating how genes regulate are regulated changing resource allocation model system unconventional life-history strategy, aim enhance our understanding importance role regulating patterns, broadening scope this discussion encompass wide range organisms.

Language: Английский

Citations

2
Molecular basis of the glycosomal targeting of PEX11 and its mislocalization to mitochondrion in trypanosomes DOI Creative Commons
Chethan K. Krishna, Nadine Schmidt,

Bettina Tippler

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Aug. 17, 2023

PEX19 binding sites are essential parts of the targeting signals peroxisomal membrane proteins (mPTS). In this study, we characterized PEX11, most abundant and glycosomal protein from Trypanosoma brucei Saccharomyces cerevisiae . Tb PEX11 contains two sites, one close to N-terminus (BS1) a second in proximity first transmembrane domain (BS2). The N-terminal BS1 is highly conserved across different organisms required for maintenance steady-state concentration efficient peroxisomes glycosomes both baker’s yeast site its localization. Deletion or mutations Sc results mislocalization mitochondria. Bioinformatic analysis indicates that region an amphiphilic helix several putative TOM20 recognition motifs. We show extreme cryptic signal directs mitochondrion if transport blocked.

Language: Английский

Citations

2
Computational Evaluation of Peroxisomal Targeting Signals in Metazoa DOI
Markus Kunze

Methods in molecular biology, Journal Year: 2023, Volume and Issue: unknown, P. 391 - 404

Published: Jan. 1, 2023

Language: Английский

Citations

1
Discovering predisposing genes for hereditary breast cancer using deep learning DOI Creative Commons

Gal Passi,

Sari Lieberman,

Fouad Zahdeh

et al.

Briefings in Bioinformatics, Journal Year: 2024, Volume and Issue: 25(4)

Published: May 23, 2024

Abstract Breast cancer (BC) is the most common malignancy affecting Western women today. It estimated that as many 10% of BC cases can be attributed to germline variants. However, genetic basis majority familial has yet identified. Discovering predisposing genes contributing challenging due their presumed rarity, low penetrance, and complex biological mechanisms. Here, we focused on an analysis rare missense variants in a cohort 12 families Middle Eastern origins characterized by high incidence cases. We devised novel, high-throughput, variant pipeline adapted for family studies, which aims analyze at protein level employing state-of-the-art machine learning models three-dimensional structural analysis. Using our pipeline, analyzed 1218 are shared between affected members classified 80 candidate pathogenic. Among these genes, found significant functional enrichment peroxisomal mitochondrial pathways segregated across seven study covered diverse ethnic groups. present multiple evidence play important, underappreciated, role both predisposition survival.

Language: Английский

Citations

0