Stress Biology,
Journal Year:
2025,
Volume and Issue:
5(1)
Published: Feb. 27, 2025
Abstract
Salinity
profoundly
influences
the
physiological
and
biochemical
well-being
of
shrimp
in
aquaculture,
dictating
their
growth,
survival,
overall
health.
In
this
study,
research
exposed
Penaeus
monodon
to
three
different
salinity
environments
(salinity
5,
20,
30)
for
62
days,
which
affected
growth
development
gut
hepatopancreas.
Further
omics
analyses
revealed
that
high
levels
led
predominance
Proteobacteria
Cyanobacteria
microbiome,
enhancing
osmoregulatory
immune
functions
essential
recovery
under
stress.
The
transcriptome
highlighted
upregulation
genes
involved
lipid
metabolism
amino
acid
processing,
emphasizing
critical
role
dynamics
adaptation.
Metabolomics
alterations
membrane
composition,
osmoprotectant
synthesis,
antioxidative
defense
mechanisms,
are
crucial
maintaining
cellular
integrity
Integrated
analysis
a
comprehensive
molecular
framework
resilience
P.
fluctuations,
underscoring
as
core
its
adaptation
strategy.
This
study
enriches
understanding
regulation
,
providing
practical
insights
low-salinity
aquaculture
responding
environmental
challenges.
Graphical
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(10), P. 1836 - 1836
Published: Oct. 7, 2023
Salinity
stress
can
trigger
a
series
of
physiological
changes.
However,
the
mechanism
underlying
response
to
acute
salinity
in
Macrobrachium
rosenbergii
remains
poorly
understood.
In
this
study,
osmoregulation,
metabolism,
antioxidant
capacity,
and
apoptosis
were
examined
over
96
h
stress.
Hemolymph
osmolality
increased
with
increasing
salinity.
After
48
exposure,
glucose,
triglycerides,
total
protein,
cholesterol
contents
two
groups
(13
26‰
salinity)
significantly
lower
than
those
0‰
group.
The
highest
levels
these
parameters
detected
at
6
h;
however,
superoxide
dismutase
(SOD),
capacity
(T-AOC),
malondialdehyde
(MDA)
lowest
13‰
activity
immunity-related
enzyme
alkaline
phosphatase
(AKP)
showed
decreasing
trend
remained
low
level
group
throughout
experiment.
No
significant
differences
observed
aspartate
aminotransferase
(AST),
alanine
(ALT),
or
lysozyme
(LZM)
among
three
treatments
h.
treatments,
gill
filament
diameter
decreased,
more
pronounced
terminal
deoxynucleotidyl
transferase
dUTP
nick
end
labeling
(TUNEL)-positive
signal
was
compared
that
Expression
apoptosis-related
genes,
including
Cysteine-aspartic
acid
protease
3
(Caspase
3),
8
8),
Cytochrome
c
(Cyt-c),
tumor
suppressor
gene
(P53),
Nuclear
factor
kappa-B
(NF-κB),
B
cell
lymphoma
2
ovarian
killer
(Bok)
higher
other
24
h,
but
Cyt-c
P53
exhibited
positive
relationship
MDA,
AST,
LZM
during
group,
Bok
expression
correlated
SOD,
T-AOC,
AKP,
phosphatase,
activity,
whereas
AST
content
positively
Caspase
8,
Cyt-c,
expression.
A
negative
between
catalase
(CAT)
activity.
These
findings
provide
insight
into
mechanisms
will
contribute
improving
M.
aquaculture
management
practices.
Frontiers in Marine Science,
Journal Year:
2023,
Volume and Issue:
9
Published: Feb. 8, 2023
Salinity
is
an
ecological
factor
affecting
the
physiology,
survival,
and
distribution
of
crustaceans.
Additionally,
salinity
fluctuation
detrimentally
affects
composition
biological
process
As
a
significant
commercial
aquaculture
species
in
China,
Japan,
Southeast
Asian
countries,
oriental
river
prawn,
Macrobrachium
nipponense,
can
tolerate
wide
range
salinity.
The
transcriptome,
proteome,
histology,
physiology
analysis
were
utilized
to
explore
physiological
responses
molecular
mechanisms
tolerance
M.
nipponense
.
Through
three-month
culture,
statistic
growth
trait
illustrated
relatively
excellent
performance
low
salinity,
higher
exposure
significantly
affected
In
terms
histological
analysis,
gills
hepatopancreas
suffered
varying
degrees
damage.
Besides,
activities
digestive,
immune-related,
metabolic
enzymes
calculated.
These
results
indicated
that
influenced
trypsin
amylase
hepatopancreas,
especially
14
ppt.
immune-related
activated
high
Notably,
activity
was
7
ppt,
which
testified
ppt
near
isotonic
point
gills,
muscle,
high-throughput
mRNA
sequencing
revealed
11356,
2227,
1819
differentially
expressed
genes
(DEGs)
by
comparing
7,
14,
21
groups
with
0ppt
group,
respectively.
TMT-labeling
proteome
identified
439
230
proteins
(DEPs)
through
comparison
0
integration
transcriptome
several
pathways
related
adaptation
enriched,
including
protein
export,
cGMP-PKG
signaling
pathway,
Amino
sugar
nucleotide
metabolism,
Glycine,
serine
threonine
metabolism.
16
up
down-regulated
DEGs
detected
KEGG
enrichment
ETHE1,
BIP,
chitinase
(E3.2.1.14),
SARDH.
no
regulated
recorded
correlation
hepatopancreas.
Thus,
optimum
survival
may
from
Overall,
these
provide
valuable
insights
into
underlying
culture
different
BMC Genomics,
Journal Year:
2023,
Volume and Issue:
24(1)
Published: June 20, 2023
Abstract
Background
Oriental
river
prawn
(
Macrobrachium
nipponense
)
is
one
of
the
most
dominant
species
in
shrimp
farming
China,
which
a
rich
source
protein
and
contributes
to
significant
impact
on
quality
human
life.
Thus,
more
complete
accurate
annotation
gene
models
are
important
for
breeding
research
oriental
prawn.
Results
A
full-length
transcriptome
muscle
was
obtained
using
PacBio
Sequel
platform.
Then,
37.99
Gb
subreads
were
sequenced,
including
584,498
circular
consensus
sequences,
among
512,216
full
length
non-chimeric
sequences.
After
Illumina-based
correction
long
reads,
6,599
error-corrected
isoforms
identified.
Transcriptome
structural
analysis
revealed
2,263
2,555
alternative
splicing
(AS)
events
polyadenylation
(APA)
sites,
respectively.
In
total,
620
novel
genes
(NGs),
197
putative
transcription
factors
(TFs),
291
non-coding
RNAs
(lncRNAs)
Conclusions
summary,
this
study
offers
insights
into
complexity
diversity
species,
provides
valuable
information
understanding
genomic
structure
improving
draft
genome
Animal Biotechnology,
Journal Year:
2024,
Volume and Issue:
35(1)
Published: April 23, 2024
The
heat
shock
protein
70
(HSP70)
gene
family
plays
a
crucial
role
in
the
response
of
organisms
to
environmental
stress.
However,
it
has
not
been
systematically
characterized
shrimp.
In
this
study,
we
identified
25
