MOMAST® Downregulates AQP3 Expression and Function in Human Colon Cells DOI Creative Commons

Ines Angelini,

Mariangela Centrone, Giusy Rita Caponio

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 26 - 26

Published: Dec. 28, 2024

The water channel AQP3 is an aquaglyceroporin expressed in villus epithelial cells, and it plays a role transport across human colonic surface cells. Beyond water, can mediate glycerol H2O2 transport. Abnormal expression function of have been found various diseases often characterized by altered cell growth proliferation. Here, the beneficial effects MOMAST® evaluated. antioxidant-patented natural phenolic complex obtained from olive wastewater (OWW) Coratina cultivar. Treatment colon HCT8 cells with reduced viability. Confocal studies Western Blotting analysis demonstrated that treatment significantly decreased staining AQP3. Importantly, functional revealed reduction abundance correlates significant decrease uptake. Indeed, was partially but presence or DFP00173, selective inhibitor In addition, MOMAST®-induced associated epithelial-mesenchymal transition (EMT)-related proteins such as vimentin β-catenin. Together, these findings propose potential adjuvant abnormal targeting

Language: Английский

Investigating the biological significance of the TCM principle “promoting urination to regulate bowel movements” through the influence of the intestinal microbiota and their metabolites on the renal-intestinal axis DOI Creative Commons
Donglin Yu, Junxi Shen, Liwen Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 10, 2025

Treatment methods in traditional Chinese medicine (TCM) are foundational to their theoretical, methodological, formulaic, and pharmacological systems, significantly contributing syndrome differentiation therapy. The principle of "promoting urination regulate bowel movements" is a common therapeutic approach TCM. core concept the dispersion drainage water dampness, regulating relieve diarrhea," yet its scientific underpinning remains unclear. Modern medical treatment for watery diarrhea primarily focuses on electrolyte replenishment, as diuretics may lead dehydration other side effects. Some reports suggest that this TCM lacks validity. Microecology, an area associated with origins TCM, closely related development, diagnosis, diarrhea. renal-intestinal axis offers molecular biological basis examining pathological mechanisms, advancing targets such "treating intestine address kidney issues" highlighting interactions within "renal-intestinal microbiota-liquid metabolism" framework, thus providing endogenous mechanism support through kidney." An increasing number studies have shown intestinal microbiota metabolites, unique mediators, involved physiological changes body. Therefore, study explores relationship between fluid metabolism from perspective aiming elucidate mechanisms underlying clarify treating via axis. This research provides new insights microbiology.

Language: Английский

Citations

0
Poly-γ-glutamic acid alleviates slow transit constipation by regulating aquaporin and gut microbes DOI Creative Commons
Xiaoru Wang, Jie Zhou, Zhang‐Hua Sun

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 10, 2025

Slow transit constipation (STC) is a prevalent gastrointestinal disorder caused by colon dysfunction. Poly-γ-glutamic acid (γ-PGA), an anionic polymer known for its moisture retention, degradability, and food safety, was studied effects on loperamide-induced STC in mice. Treatment with γ-PGA one week significantly increased both defecation frequency fecal water content, the high-dose group (10 g/kg/d) restoring content to 34.23%, outperforming low- (16.16%) medium-dose (27.08%) groups exceeding positive control, PEG, 1.35 times. enhanced intestinal peristalsis reduced expression of inflammatory markers (IL-1β, IL-6, caspase-1, TLR2) water-electrolyte transport genes (AQP3, AQP4, ENaC-β), while improving tight junction proteins (Claudin-1, Occludin, ZO-1) damaged loperamide. Histopathological analyses confirmed γ-PGA's capacity repair damage. Additionally, Western Blot analysis indicated AQP3/4 levels colon, molecular docking showed good binding affinity between AQPs. also positively altered gut microbiota composition. Overall, shows promise treating modulating aquaporins microbiota.

Language: Английский

Citations

0
Exploring the Shared Diagnostic Genes in IBD and Psoriasis through Bioinformatics and Experimental Assays DOI Creative Commons

Lichun Han,

Guangfu Lin,

Xiaodan Lv

et al.

International Journal of Medical Sciences, Journal Year: 2025, Volume and Issue: 22(7), P. 1680 - 1697

Published: March 3, 2025

Background: Inflammatory bowel disease (IBD) is a persistent, non-specific inflammation affecting the intestines. Psoriasis long-lasting inflammatory disorder of skin. There comorbidity correlation between IBD and psoriasis, but specific pathogenesis unclear. Materials methods: In this study, we analyzed datasets sourced from Gene Expression Omnibus (GEO) database, identified shared genes psoriasis through differential expression analysis weighted gene co-expression network (WGCNA). Then three machine learning algorithms were applied to identify diagnostic genes. Next, validation was evaluated with ROC curves, AUC determined. Subsequently, single sample set enrichment (ssGSEA) immune infiltration conducted. Furthermore, obtained potential drugs such as securinine in Drug Signature Database (DsigDB) 7 traditional Chinese medicines Coremine which might have therapeutic effects on psoriasis. Finally, confirmed colitis mice tissues RT-PCR, Western blot immunohistochemistry (IHC) methods. Results: The results showed that AQP9 had highest value for two diseases. values 93.681% UC, 89.629% CD,and 78.689% internal datasets. external datasets, 90.394% 93.909% 82.906% Immune ssGSEA revealed impact process by participating NF-kappaB signaling pathway, modulating cell differentiation. levels consistently validated, showing upregulation downregulation compared control group. Conclusions: This study mechanisms providing new directions future research exploring treatment targets.

Language: Английский

Citations

0
The Emerging Role of Water Loss in Dog Aging DOI Creative Commons
Gabriella Guelfi, Camilla Capaccia,

Vicente Francisco Ratto

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(7), P. 545 - 545

Published: April 4, 2025

Aging involves progressive physiological changes, including the dysregulation of water homeostasis, essential for cellular function, neuronal signaling, and musculoskeletal integrity. This review explores emerging role loss as a central underestimated driver functional decline in aging, with focus on dog, both clinically relevant target species model human aging. Age-related alterations metabolism—driven by changes body composition, aquaporin (AQP) expression, electrolyte imbalances, reduced thirst perception, impaired urine concentration—lead to intracellular extracellular dehydration, exacerbating decline. We examine molecular mechanisms regulation involving AQPs osmolytes, describe how dehydration contributes structural metabolic dysfunction across key biological compartments, kidney, brain, bone, skeletal muscle. Physiological hallmark intensifies inflammaging, accelerating tissue degeneration. In particular, we highlight impairs solvent capacity, solute transport, protein conformation, communication. Despite known macronutrients geriatric nutrition, hydration remains an often-overlooked factor aging management. argue its inclusion fourth pillar nutritional approach veterinary geriatrics, alongside protein, fat, fiber. By investigating aging-associated dogs—species that share environments lifestyle patterns humans—we propose hydration-centered strategies promote healthy comparative medicine.

Language: Английский

Citations

0
Relief of loperamide-induced constipation by peptidic and small molecule RXFP4 agonists in mice DOI

Shiyu Yan,

Yan Chen, Jiang Wang

et al.

Biochemical Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 116924 - 116924

Published: April 1, 2025

Language: Английский

Citations

0
Flavonoids from Cerasus humilis seed coat alleviate constipation in mice: Role of AQP3/4 and c-Kit/SCF signaling pathways DOI
Qiang Xiao, Jinjiang Zhao,

Zongshuo Li

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106643 - 106643

Published: April 1, 2025

Language: Английский

Citations

0
Distinctive roles of aquaporins and novel therapeutic opportunities against cancer DOI
Dharmendra Kumar Yadav, Desh Deepak Singh, Dongyun Shin

et al.

RSC Medicinal Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Aquaporins (AQPs) are integral membrane proteins responsible for facilitating the transmembrane transport of water and small solutes.

Language: Английский

Citations

0
MOMAST® Downregulates AQP3 Expression and Function in Human Colon Cells DOI Creative Commons

Ines Angelini,

Mariangela Centrone, Giusy Rita Caponio

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 14(1), P. 26 - 26

Published: Dec. 28, 2024

The water channel AQP3 is an aquaglyceroporin expressed in villus epithelial cells, and it plays a role transport across human colonic surface cells. Beyond water, can mediate glycerol H2O2 transport. Abnormal expression function of have been found various diseases often characterized by altered cell growth proliferation. Here, the beneficial effects MOMAST® evaluated. antioxidant-patented natural phenolic complex obtained from olive wastewater (OWW) Coratina cultivar. Treatment colon HCT8 cells with reduced viability. Confocal studies Western Blotting analysis demonstrated that treatment significantly decreased staining AQP3. Importantly, functional revealed reduction abundance correlates significant decrease uptake. Indeed, was partially but presence or DFP00173, selective inhibitor In addition, MOMAST®-induced associated epithelial-mesenchymal transition (EMT)-related proteins such as vimentin β-catenin. Together, these findings propose potential adjuvant abnormal targeting

Language: Английский

Citations

0