Investigating the potential role of the pituitary adenylate cyclase-activating polypeptide (PACAP) in regulating the ubiquitin signaling pathway in poultry

General and Comparative Endocrinology, Journal Year: 2024, Volume and Issue: 356, P. 114577 - 114577

Published: June 22, 2024

The physiological processes in animal production are regulated through biologically active molecules like peptides, proteins, and hormones identified the development of fundamental sciences their application. One main polypeptides that plays an essential role regulating responses is pituitary adenylate cyclase-activating polypeptide (PACAP). PACAP belongs to glucagon/growth hormone-releasing hormone (GHRH)/vasoactive intestinal proteins (VIP) family regulates feed intake, stress, immune response birds. Most these regulations occur after stimulates cAMP signaling pathway, which can regulate expression genes MuRF1, FOXO1, Atrogin 1, other ligases members ubiquitin system. On hand, secretion CRH activating pathway a vital protein degradation oxidative stress responses. Many studies conducted on rodents, mammals, models confirm regulatory effects PACAP, cAMP, pathway; however, there no testing whether PACAP-induced poultry pathway. Besides, it would be interesting investigate if during via altered by HPA axis stimulation. Therefore, this review highlights summary research indicate potential interaction pathways different molecular parameters species pathways.

Language: Английский

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Network pharmacology, computational biology integrated surface plasmon resonance technology reveals the mechanism of salidroside in alleviating diabetic amyotrophy

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

Objective To explore the target and mechanism of Salidroside (SDS) in treatment Diabetic amyotrophy (DPN) employing network pharmacology, computational biology, surface plasmon resonance verification. Method: The associated with SDS was acquired from ChEmBL database DPN-related targets were obtained GeneCards database. Relevant imported into Venny platform to generate a Venn diagram, their intersections visualized. protein-protein interaction (PPI) constructed using STRING, DAVID database, Cytoscape software, core screened. After subjecting GO enrichment KEGG pathway analysis, "target-pathway for alleviating DPN" set up. Schrodinger Maestro 13.5 software utilized molecular docking order ascertain binding free energy mode between proteins. Molecular dynamics simulations performed Desmond program. Saturation mutation analysis Schrodinger's software. Finally, SPR technology used affinity Caspase3 protein. Results Network pharmacological showed that there total 61 intersection proteins, among which TNF, APP, Caspase3, PPARG, NQO1, HDAC1, BCL2, SRC, HDAC6, ACE, MAPK3, HSP90AA1, ATM, REN potential alleviate DPN. function pathways revealed primarily participated diverse biological processes, cellular components alteraions, functions apoptosis, neurons transmitters, as well metabolic involved lipid atherosclerosis, neurodegenerative pathways. Based on crystal structure protein, complex model target-SDS created (XP flexible docking), MMGBS carried out. simulation Δaffinity highest 206 (TRP→GLY), (TRP→LYS), (TRP→ALA). corresponding values 10.847 kcal/mol, 10.008 9.725 kcal/mol. results data demonstrated specific kinetic compatibility Conclusion is DPN may eventually play role by regulating apoptosis-related providing theoretical basis along clues research development anti-alleviating drugs.

Language: Английский

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Utility of urinary N-titin as a muscle atrophy biomarker in dexamethasone-induced muscle atrophy model mice

Fundamental Toxicological Sciences, Journal Year: 2024, Volume and Issue: 11(4), P. 159 - 168

Published: Jan. 1, 2024

Titin is a giant protein that specifically expressed in striated muscle and essential for the maintenance of sarcomere structure function. Recently, N-terminal fragment (N-titin) has been reported to show high levels human urine patients with muscular diseases expected serve as diagnostic biomarker these diseases. In this study, we examined utility N-titin detect atrophy mice. Male BALB/c mice (6 weeks age, n=5 per group) were given 10 mg/L dexamethasone (DEX) dissolved drinking water 4 weeks. The gastrocnemius (GAS) weight was significantly decreased mRNA atrophy-related genes (Atrogin-1 MuRF-1) increased GAS after DEX treatment. Although there no degenerative/necrotic changes histopathological examination, fiber cross-sectional area GAS. On other hand, treatment-related weights soleus muscle. These results suggest 4-week treatment preferentially caused fast-dominant Under condition urinary N-titin/CRN ratio markedly from Week 2 From above results, could be monitoring skeletal

Language: Английский

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Ubiquitination Insight from Spinal Muscular Atrophy—From Pathogenesis to Therapy: A Muscle Perspective

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8800 - 8800

Published: Aug. 13, 2024

Spinal muscular atrophy (SMA) is one of the most frequent causes death in childhood. The disease’s molecular basis deletion or mutations SMN1 gene, which produces reduced survival motor neuron protein (SMN) levels. As a result, there spinal degeneration and large increase muscle atrophy, ubiquitin–proteasome system (UPS) plays significant role. In humans, paralogue SMN1, SMN2 encodes truncated SMNΔ7. Structural differences between SMN SMNΔ7 affect interaction proteins with UPS decrease stability protein. loss affects general ubiquitination process by lowering levels UBA1, main enzymes process. We discuss how both process, involved could be used as future targets for SMA treatment.

Language: Английский

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Letter to the Editor: Acute Skeletal Muscle Wasting is Associated with Prolonged Hospital Stay in Critical Illness with Brain Injury

Neurocritical Care, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 23, 2024

Language: Английский

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